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    USING TETHERED ENZYMES TO DETECT NUCLEIC ACIDS
    4.
    发明申请
    USING TETHERED ENZYMES TO DETECT NUCLEIC ACIDS 有权

    公开(公告)号：US20220136040A1

    公开(公告)日：2022-05-05

    申请号：US17434065

    申请日：2020-02-26

    Applicant: CORNELL UNIVERSITY

    Inventor： Roy COHEN ,  Alexander TRAVIS

    IPC: C12Q1/6825 ,  G01N21/76

    Abstract: The present application relates to methods of detecting a target nucleic acid molecule in a sample. The method includes providing a sample containing a target nucleic acid molecule and a capture oligonucleotide molecule. In one embodiment, the capture oligonucleotide molecule has (i) a length of 30-60 base pairs, (ii) a 4-8 base pair overhang on its 3′ end, (iii) a 5′ tail, (iv) a target-specific portion between the 3′ end and the 5′ tail, (v) a deoxy-adenosine diphosphate content of 40-50%, (vi) no deoxy thymidine phosphate in the 3′ end or the 5′ tail, and (vii) the 3′ end and the 5′ tail having an ATP content which is 40-50% of that of the capture oligonucleotide molecule. In another aspect of the method of detecting, certain reagents are coupled to a solid support. The present application also relates to compositions and kits useful in carrying out the methods of the present application.

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