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    ENZYME COMPOSITION FOR DNA END REPAIR, ADENYLATION, PHOSPHORYLATION
    3.
    发明申请
    ENZYME COMPOSITION FOR DNA END REPAIR, ADENYLATION, PHOSPHORYLATION 有权
    用于DNA末端修复的酶组合物，腺嘌呤，磷酸化

    公开(公告)号：US20150087557A1

    公开(公告)日：2015-03-26

    申请号：US14494684

    申请日：2014-09-24

    Applicant: Thermo Fisher Scientific Baltics UAB

    Inventor： Judita LUBIENE ,  Arturas Berezniakovas ,  Arvydas Lubys

    IPC: C12Q1/68 ,  C12N9/96 ,  C12N9/12

    CPC classification number: C12N9/96 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/1252 ,  C12N9/22 ,  C12N11/18 ,  C12Y207/01078

    Abstract: Enzyme compositions and their method of use that provide ready-to-use master mixtures. The compositions comprise a modified thermophilic DNA polymerase lacking 5′-3′ and 3′-5′ exonuclease activity premixed with T4 DNA polymerase, Klenow fragment and T4 polynucleotide kinase and all other necessary components, including reaction buffer and nucleoside triphosphates, required to perform DNA blunting, phosphorylation, and single nucleotide extension reactions in one tube and in two steps. Among other benefits, the mixture of different enzymes, buffers and nucleoside triphosphates is stable during prolonged storage.

    Abstract translation: 提供即用型主混合物的酶组合物及其使用方法。 组合物包含缺乏与T4DNA聚合酶，Klenow片段和T4多核苷酸激酶以及所有其它必需组分（包括反应缓冲液和核苷三磷酸）预混合的5'-3'和3'-5'外切核酸酶活性的修饰的嗜热DNA聚合酶，其执行所需 DNA钝化，磷酸化和单核苷酸延伸反应在一个管和两个步骤。 除了其他好处之外，不同酶，缓冲液和三磷酸核苷酸的混合物在长期储存期间是稳定的。

    Method of immobilizing malate dehydrogenase on a substrate
    4.
    发明申请
    Method of immobilizing malate dehydrogenase on a substrate 有权
    将苹果酸脱氢酶固定在底物上的方法

    公开(公告)号：US20080050765A1

    公开(公告)日：2008-02-28

    申请号：US11976951

    申请日：2007-10-30

    Applicant: Hidenobu Yaku ,  Hirokazu Sugihara

    Inventor： Hidenobu Yaku ,  Hirokazu Sugihara

    IPC: C12Q1/32 ,  C12N11/04

    CPC classification number: C12Q1/32 ,  C12N9/0006 ,  C12N11/18 ,  C12Q1/003 ,  C12Y101/01037 ,  G01N33/54393

    Abstract: Immobilization of malate dehydrogenase on a substrate using a glycerol solution containing malate dehydrogenase is achieved through dropping a mixed solution obtained by adding at least one selected from malic acid and malate to the glycerol containing malate dehydrogenase on the substrate, and drying it thereon. It is preferable to prepare the mixed solution by adding the malate to the glycerol solution containing malate dehydrogenase. The malate is preferably at least one selected from potassium malate and sodium malate.

    Abstract translation: 使用含有苹果酸脱氢酶的甘油溶液将底物上的苹果酸脱氢酶固定在底物上，通过将通过将选自苹果酸和苹果酸中的至少一种的至少一种添加到底物上的甘油含苹果酸脱氢酶中而得到的混合溶液进行干燥。 优选通过将苹果酸盐加入到含有苹果酸脱氢酶的甘油溶液中来制备混合溶液。 苹果酸盐优选为选自苹果酸钾和苹果酸钾中的至少一种。

    Immobilization of penicillin G amidase, glutaryl-7-ACA acylase or D-aminoacid oxidase on an aminofunctional organosiloxane polymer carrier
    8.
    发明授权
    Immobilization of penicillin G amidase, glutaryl-7-ACA acylase or D-aminoacid oxidase on an aminofunctional organosiloxane polymer carrier 失效
    青霉素G酰胺酶，戊二酰-7-ACA酰基转移酶或D-氨基酸氧化酶固定在氨基官能有机硅氧烷聚合物载体上

    公开(公告)号：US5780260A

    公开(公告)日：1998-07-14

    申请号：US648015

    申请日：1996-05-30

    Applicant: Frank Wedekind ,  Adelheid Daser ,  Wilhelm Tischer

    Inventor： Frank Wedekind ,  Adelheid Daser ,  Wilhelm Tischer

    IPC: C12N11/06 ,  C12N9/06 ,  C12N9/84 ,  C12N11/08 ,  C12N11/14 ,  C12N11/18 ,  C12P37/00 ,  C12P35/00

    CPC classification number: C12N11/18 ,  C12N11/08 ,  C12N11/14

    Abstract: An enzyme selected from penicillin-G amidase, glutaryl-7-ACA acylase and D-amino acid oxidase is immobilized by covalent bonding on an aminofunctional organosiloxane polymer carrier to provide an immobilized enzyme having a specific volume activity of at least 100 U/g wet carrier. Preferably, the carrier has an average diameter or 0.01 to 3 mm and is essentially spherical. Covalent bonding is accomplished by activating amino groups on the carrier with a dialdehyde and reacting the activated groups with reactive groups on the enzyme. An amount of enzyme is immobilized to provide a weight ratio of enzyme to carrier of 1 to 300 mg protein per g wet carrier.

    Abstract translation: PCT No.PCT / EP94 / 04132 Sec。 371日期：1996年5月30日 102（e）日期1996年5月30日PCT 1996年12月13日PCT公布。 WO95 / 16773 PCT出版物 日期：1995年6月22日通过共价键在氨基官能的有机硅氧烷聚合物载体上固定选自青霉素-G酰胺酶，戊二酰-7-ACA酰基转移酶和D-氨基酸氧化酶的酶，以提供至少具有特定体积活性的固定化酶 100 U / g湿载体。 优选地，载体具有平均直径或0.01至3mm，并且基本上是球形的。 通过用二醛活化载体上的氨基并使活化基团与酶上的反应性基团反应来实现共价键合。 固定一定量的酶以提供酶与载体的重量比为1至300mg蛋白/ g湿载体。

    Method of binding enzymes to sintered expanded clays
    10.
    发明授权
    Method of binding enzymes to sintered expanded clays 失效
    将酶与烧结膨胀粘土结合的方法

    公开(公告)号：US5443975A

    公开(公告)日：1995-08-22

    申请号：US986045

    申请日：1992-12-10

    Applicant: Stefano Cervelli ,  Vincenzo Capuano

    Inventor： Stefano Cervelli ,  Vincenzo Capuano

    IPC: C02F3/10 ,  C02F3/34 ,  C12N11/14 ,  C12N11/18 ,  C12N11/04 ,  C12N11/08

    CPC classification number: C02F3/108 ,  C02F3/10 ,  C02F3/342 ,  C12N11/14 ,  C12N11/18 ,  Y02W10/15

    Abstract: Enzymes, cells and/or cellular organelles are bound to an insoluble sintered expanded clay support matrix for catalyzing transformations of agriculture and industrial residues in soil and in other environments. In one embodiment, an enzyme is absorbed by the sintered expanded clay support matrix and a phenolic monomer is polymerized or copolymerized on the support matrix containing the bound enzyme. In another embodiment, an enzyme different from the enzyme absorbed by the support matrix is combined with the phenolic monomer and a copolymer of enzyme and phenolic monomer is formed on the support matrix containing the bound enzyme. The phenolic monomer is preferably catechol, pyrogallic acid and/or resorcinol. Polymerization or copolymerization of the phenolic monomer is catalyzed by enzyme catalysis with laccase or tyrosinase or peroxidase in combination with hydrogen peroxide, by inorganic catalysis with ammonium persulfate or manganese dioxide, or by organic catalysis with N,N,N',N' tetramethyl-ethylene-diamine (TEMED).

    Abstract translation: 酶，细胞和/或细胞器器结合于不溶性烧结膨胀粘土支撑基质，用于催化土壤和其它环境中农业和工业残留物的转化。 在一个实施方案中，酶被烧结的膨胀粘土载体基质吸收，并且酚类单体在含有结合酶的载体基质上聚合或共聚。 在另一个实施方案中，将不同于由载体基质吸收的酶的酶与酚类单体组合，并且在含有结合酶的载体基质上形成酶和酚单体的共聚物。 酚类单体优选为邻苯二酚，焦性焦酸和/或间苯二酚。 酚类单体的聚合或共聚通过用漆酶或酪氨酸酶或过氧化物酶与过氧化氢组合的酶催化，通过用过硫酸铵或二氧化锰的无机催化或通过有机催化N，N'，N'-四甲基 - 乙二胺（TEMED）。

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