公开(公告)号：US11078480B2

公开(公告)日：2021-08-03

申请号：US16575124

申请日：2019-09-18

Applicant: California Institute of Technology

Inventor： Mitchell Guttman ,  Sofia A. Quinodoz ,  Mario R. Blanco ,  Devdoot Majumdar

IPC: C12N15/10 ,  G01N33/53 ,  G01N33/68

Abstract: Methods of detecting an interaction between a macromolecule and an interaction partner are described. Kits are also described.

公开(公告)号：US20180100863A1

公开(公告)日：2018-04-12

申请号：US15729253

申请日：2017-10-10

Applicant: California Institute of Technology

Inventor： Devdoot Majumdar ,  Anirudh Mathukumilli ,  David Baltimore

IPC: G01N33/68 ,  C12Q1/68 ,  C12N15/10

CPC classification number: G01N33/6854 ,  C12N15/1037 ,  C12N15/1055 ,  C12Q1/6876 ,  G01N33/6878

Abstract: Provided herein are method and kits for elucidation of immunological repertoires (comprising functional pairs of immune cell receptors and antigens). In some embodiments, an immune cell receptor and an antigen that it binds to are isolated, and sequence information is obtained about the immune cell receptor and antigen. In some embodiments, an antigen expressed on a first cell and an immune cell receptor expressed on a second cell can form a synapse, mRNAs can be isolated from the resulting doublet of cells, and sequences of the functional immune cell receptor and its antigen can be obtained from the mRNAs.

公开(公告)号：US10739351B2

公开(公告)日：2020-08-11

申请号：US15729253

申请日：2017-10-10

Applicant: California Institute of Technology

Inventor： Devdoot Majumdar ,  Anirudh Mathukumilli ,  David Baltimore

IPC: G01N33/68 ,  C12N15/10 ,  C12Q1/6876

Abstract: Provided herein are method and kits for elucidation of immunological repertoires (comprising functional pairs of immune cell receptors and antigens). In some embodiments, an immune cell receptor and an antigen that it binds to are isolated, and sequence information is obtained about the immune cell receptor and antigen. In some embodiments, an antigen expressed on a first cell and an immune cell receptor expressed on a second cell can form a synapse, mRNAs can be isolated from the resulting doublet of cells, and sequences of the functional immune cell receptor and its antigen can be obtained from the mRNAs.

公开(公告)号：US20200087657A1

公开(公告)日：2020-03-19

申请号：US16575124

申请日：2019-09-18

Applicant: California Institute of Technology

Inventor： Mitchell Guttman ,  Sofia A. Quinodoz ,  Mario R. Blanco ,  Devdoot Majumdar

IPC: C12N15/10 ,  G01N33/68 ,  G01N33/53

Abstract: Methods of detecting an interaction between a macromolecule and an interaction partner are described. Kits are also described.