公开(公告)号：US09657317B2

公开(公告)日：2017-05-23

申请号：US14344899

申请日：2012-10-17

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Tom Purcell ,  Jon Edward Ness ,  Effendi Leonard

IPC: C12P7/42 ,  C12P19/32 ,  C12P7/40 ,  C12N15/52

CPC classification number: C12P7/42 ,  C12N15/52 ,  C12P7/40 ,  C12P19/32

Abstract: Provided herein is an alkane-metabolizing cell that is unable to convert propionyl-CoA into methylmalonyl-CoA or 2-metylcitrate synthase. Depending on which enzymes are present in the cell, the cell can produce acrylate or a precursor for the same (e.g., propionate, 3-hydroxypropionyl-CoA, 3-hydroxypropionate, acrylyl-CoA) that can be readily converted to acrylate enzymatically (e.g., in the cell) or by chemical treatment. In one embodiment, the cell may contain a cytochrome P450 or alkane oxidase enzyme that allows the production of 3-hydroxypropionyl-CoA, which can be readily converted to 3-hydroxypropionate. In order to make such compounds, the cell may be grown in the presence of an odd-numbered chain alkane (e.g., pentane or heptane), although another odd-numbered chain alkane may be used. In another embodiment, the cell may contain acyl-CoA oxidase, enoyl-CoA hydratase, and hydrolase.

公开(公告)号：US20150087036A1

公开(公告)日：2015-03-26

申请号：US14344899

申请日：2012-10-17

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Tom Purcell ,  Jon Edward Ness ,  Effendi Leonard

IPC: C12P7/42 ,  C12P7/40

CPC classification number: C12P7/42 ,  C12N15/52 ,  C12P7/40 ,  C12P19/32

Abstract: Provided herein is an alkane-metabolizing cell that is unable to convert propionyl-CoA into methylmalonyl-CoA or 2-metylcitrate synthase. Depending on which enzymes are present in the cell, the cell can produce acrylate or a precursor for the same (e.g., propionate, 3-hydroxypropionyl-CoA, 3-hydroxypropionate, acrylyl-CoA) that can be readily converted to acrylate enzymatically (e.g., in the cell) or by chemical treatment. In one embodiment, the cell may contain a cytochrome P450 or alkane oxidase enzyme that allows the production of 3-hydroxypropionyl-CoA, which can be readily converted to 3-hydroxypropionate. In order to make such compounds, the cell may be grown in the presence of an odd-numbered chain alkane (e.g., pentane or heptane), although another odd-numbered chain alkane may be used. In another embodiment, the cell may contain acyl-CoA oxidase, enoyl-CoA hydratase, and hydrolase.

Abstract translation: 本文提供的是不能将丙酰辅酶A转化为甲基丙二酰辅酶A或2-甲基柠檬酸合酶的烷烃代谢细胞。 根据细胞中哪些酶的存在，细胞可以生成丙烯酸酯或相同的前体（例如，丙酸酯，3-羟基丙酰基-CoA，3-羟基丙酸酯，丙烯酰辅酶A），其可以容易地酶转化成丙烯酸酯（例如 ，在细胞中）或通过化学处理。 在一个实施方案中，细胞可以含有细胞色素P450或烷烃氧化酶，其允许产生3-羟基丙酰辅酶A，其可以容易地转化为3-羟基丙酸酯。 为了制备这种化合物，可以在奇数链烷烃（例如戊烷或庚烷）的存在下生长电池，尽管可以使用另一个奇数链烷烃。 在另一个实施方案中，细胞可以含有酰基辅酶A氧化酶，烯酰辅酶A水合酶和水解酶。