公开(公告)号：US20190330292A1

公开(公告)日：2019-10-31

申请号：US16401246

申请日：2019-05-02

Applicant: DOW AGROSCIENCES, LLC

Inventor： Joseph Petolino ,  Jianquan Li ,  Steven L. Evans ,  Ryan C. Blue

IPC: C07K14/47 ,  C07K14/415 ,  C12N15/82 ,  C12N15/62

Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.

公开(公告)号：US20210087241A1

公开(公告)日：2021-03-25

申请号：US17073831

申请日：2020-10-19

Applicant: Dow AgroSciences LLC

Inventor： Joseph F. Petolino ,  Jianquan Li ,  Steven L. Evans ,  Ryan C. Blue

IPC: C07K14/47 ,  C12N15/62 ,  C07K14/415 ,  C12N15/82

Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.

公开(公告)号：US10415046B2

公开(公告)日：2019-09-17

申请号：US15338888

申请日：2016-10-31

Applicant: DOW AGROSCIENCES LLC

Inventor： W. Michael Ainley ,  James W. Bing ,  David R. Corbin ,  Steven L. Evans ,  Joseph F. Petolino ,  Lakshmi Sastry-Dent ,  Steven A. Thompson ,  Steven R. Webb ,  Mary E. Welter ,  Ning Zhou

IPC: C12N15/82 ,  C12N9/12

Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.

公开(公告)号：US20150176015A1

公开(公告)日：2015-06-25

申请号：US14416799

申请日：2013-07-23

Applicant: DOW AGROSCIENCES LLC

Inventor： Sandeep Kumar ,  Steven L. Evans ,  Manju Gupta

IPC: C12N15/82

CPC classification number: C12N15/8209 ,  C12N15/68 ,  C12N15/82

Abstract: This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.

Abstract translation: 本发明涉及用于转基因植物的载体组装的方法和系统。 使用统一的模块化方法来减少循环时间，本文提供的方法和系统可以使用多孔板（例如96孔板）提高克隆产量。 在一些实施方案中，本文提供的方法和系统消除或减少对测序确认的需要，因为在载体组装过程中不涉及PCR。

公开(公告)号：US10077449B2

公开(公告)日：2018-09-18

申请号：US14531710

申请日：2014-11-03

Applicant: Dow AgroSciences LLC

Inventor： Lakshmi Sastry-Dent ,  Steven L. Evans ,  Ryan C. Blue ,  Zehui Cao

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C12N15/8209 ,  C12N15/8212 ,  C12N15/8275

Abstract: A universal donor polynucleotide is described that can be inserted at targeted locations in plant genomes to facilitate rapid and high throughput integration of a donor molecule within a specific genomic location.

公开(公告)号：US09752154B2

公开(公告)日：2017-09-05

申请号：US14416799

申请日：2013-07-23

Applicant: DOW AGROSCIENCES LLC ,  Sandeep Kumar ,  Steven L. Evans ,  Manju Gupta

Inventor： Sandeep Kumar ,  Steven L. Evans ,  Manju Gupta

IPC: C12N15/68 ,  C12N15/82

CPC classification number: C12N15/8209 ,  C12N15/68 ,  C12N15/82

Abstract: This invention is related to methods and systems for vector assembly for transgenic plants. A uniform modular process is used to reduce cycle time and the methods and systems provided herein can increase cloning throughput using multiple-well plates, for example 96-well plates. In some embodiments, the methods and systems provided herein eliminate or reduce the need for sequencing confirmation because no PCR is involved in the vector assembly process.