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    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS
    3.
    发明申请
    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS 审中-公开
    用于植物基因工程的土地垫

    公开(公告)号：US20150040267A1

    公开(公告)日：2015-02-05

    申请号：US14458086

    申请日：2014-08-12

    Applicant: Dow AgroSciences LLC

    Inventor： William Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

    IPC: C12N15/82

    CPC classification number: C12N15/8201 ,  C12N15/8213 ,  C12N15/8241

    Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

    Abstract translation: 一种用于生产转基因植物的方法包括提供核酸分子，其包含与植物细胞的基因组DNA缺乏序列同源性的至少两个核酸序列区域和至少两个锌指核酸酶核酸酶识别位点，其中所述至少两个区域 与在至少两个锌指核酸酶识别位点相邻的植物细胞的基因组DNA缺乏序列同源性的核酸序列。 转化具有稳定整合到植物细胞基因组中的核酸分子的植物细胞或组织。 植物从植物细胞再生。 通过该方法产生转基因植物。 种子由转基因植物产生。

    PLANT TRANSACTIVATION INTERACTION MOTIFS AND USES THEREOF
    4.
    发明申请
    PLANT TRANSACTIVATION INTERACTION MOTIFS AND USES THEREOF 审中-公开

    公开(公告)号：US20190330292A1

    公开(公告)日：2019-10-31

    申请号：US16401246

    申请日：2019-05-02

    Applicant: DOW AGROSCIENCES, LLC

    Inventor： Joseph Petolino ,  Jianquan Li ,  Steven L. Evans ,  Ryan C. Blue

    IPC: C07K14/47 ,  C07K14/415 ,  C12N15/82 ,  C12N15/62

    Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.

    Engineered landing pads for gene targeting in plants
    5.
    发明授权
    Engineered landing pads for gene targeting in plants 有权

    公开(公告)号：US10160975B2

    公开(公告)日：2018-12-25

    申请号：US14458086

    申请日：2014-08-12

    Applicant: Dow AgroSciences LLC

    Inventor： W. Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

    IPC: C12N15/82

    Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS
    8.
    发明申请
    ENGINEERED LANDING PADS FOR GENE TARGETING IN PLANTS 审中-公开

    公开(公告)号：US20190085344A1

    公开(公告)日：2019-03-21

    申请号：US16209028

    申请日：2018-12-04

    Applicant: Dow AgroSciences LLC

    Inventor： William Michael Ainley ,  Ryan C. Blue ,  Michael G. Murray ,  David Richard Corbin ,  Rebecca Ruth Miles ,  Steven R. Webb

    IPC: C12N15/82

    Abstract: A method for producing a transgenic plant includes providing a nucleic acid molecule comprising at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell, and at least two zinc finger nuclease recognition sites, wherein the at least two regions of nucleic acid sequence that lack sequence homology with genomic DNA of the plant cell flank the at least two zinc finger nuclease recognition sites. A plant cell or tissue having the nucleic acid molecule stably integrated into the genome of the plant cell is transformed. A plant is regenerated from the plant cell. Transgenic plants are produced by the method. Seeds are produced by the transgenic plants.

    HAPLOID MAIZE TRANSFORMATION
    9.
    发明申请
    HAPLOID MAIZE TRANSFORMATION 审中-公开
    HAPLOID MAIZE转换

    公开(公告)号：US20150307889A1

    公开(公告)日：2015-10-29

    申请号：US14695434

    申请日：2015-04-24

    Applicant: Dow AgroSciences LLC

    Inventor： Joseph F. Petolino ,  Tonya L. Strange ,  Jayakumar Pon Samuel ,  Ryan C. Blue ,  Matthew A. Simpson

    IPC: C12N15/82

    CPC classification number: C12N15/8213

    Abstract: Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.

    Abstract translation: 公开了用位点特异性核酸酶转化雄激素衍生的单倍体细胞系的方法。 在一些实施方案中，雄激素衍生的单倍体细胞系是来自玉米小孢子的植物组织培养物。 此外，本公开提供了修饰，例如通过突变或靶向并将供体DNA整合到单倍体或双倍体组织基因组的特定基因座中的方法。 本公开进一步提供了从单倍体或双倍体组织再生整个植物的方法，所述单体或双倍体组织含有特定基因组座位上的突变或整合在特定基因组基因组中的供体DNA，可从本发明公开内容获得。

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