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    Use of a maize untranslated region for transgene expression in plants
    4.
    发明授权
    Use of a maize untranslated region for transgene expression in plants 有权

    公开(公告)号：US09688996B2

    公开(公告)日：2017-06-27

    申请号：US14416811

    申请日：2013-08-08

    Applicant: DOW AGROSCIENCES LLC ,  Sandeep Kumar ,  Manju Gupta ,  Diaa Alabed

    Inventor： Sandeep Kumar ,  Manju Gupta ,  Diaa Alabed

    IPC: C12N15/82

    CPC classification number: C12N15/8216 ,  C12N15/8218

    Abstract: Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3′ untranslated regions of genes encoding ubiquitin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

    METHOD AND CONSTRUCT FOR SYNTHETIC BIDIRECTIONAL SCBV PLANT PROMOTER
    6.
    发明申请
    METHOD AND CONSTRUCT FOR SYNTHETIC BIDIRECTIONAL SCBV PLANT PROMOTER 有权
    合成双向SCBV植物促进剂的方法和构建

    公开(公告)号：US20130198898A1

    公开(公告)日：2013-08-01

    申请号：US13674712

    申请日：2012-11-12

    Applicant: Dow AgroSciences LLC

    Inventor： Sandeep Kumar ,  Diaa Alabed ,  Sara Bennett ,  Manju Gupta ,  Susan Jayne ,  Terry R. Wright

    IPC: C12N15/82

    CPC classification number: C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

    Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

    Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包括至少一个与多个基因表达盒连接的双向启动子，其中双向启动子包含来自甘蔗芽孢杆菌病毒启动子的功能启动子核苷酸序列。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1基因或其功能等同物的最小核心启动子元件和来自甘蔗芽孢杆菌病毒启动子的核苷酸序列元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

    METHOD TO DETERMINE ZYGOSITY OF THE FAD3 GENE IN CANOLA USING END-POINT TAQMAN® PCR
    8.
    发明申请
    METHOD TO DETERMINE ZYGOSITY OF THE FAD3 GENE IN CANOLA USING END-POINT TAQMAN® PCR 有权
    使用终点TAQMAN®PCR确定CANOLA中FAD3基因的ZYGOSITY的方法

    公开(公告)号：US20130102002A1

    公开(公告)日：2013-04-25

    申请号：US13656270

    申请日：2012-10-19

    Applicant: Dow AgroSciences LLC

    Inventor： Lasantha Chandana Ubayasena ,  Zoe Ehlert ,  Manju Gupta ,  Chandra Shekara A. Channabasavaradhya

    IPC: G01N21/64

    CPC classification number: C12Q1/6895 ,  C12Q2600/156

    Abstract: The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.

    Abstract translation: 本发明的一部分涉及终点TaqMan PCR分析用于卡诺拉油菜中fad-3c基因的检测和高通量接合度分析。 本发明进一步涉及使用野生型DNA作为用于确定接合性的参考。 这些和其它相关方法可用于唯一地鉴定包含受试基因的卡诺拉系的接合度和品种。 本发明还提供了用于确定来自卡诺拉植物或种子的样品的接合性的相关试剂盒。

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