公开(公告)号：US20230250406A1

公开(公告)日：2023-08-10

申请号：US17056230

申请日：2019-05-17

Applicant: DSM IP Assets B.V.

Inventor： René Marcel DE JONG ,  Willem BIJLEVELD ,  Paul Frederick MUGFORD

IPC: C12N9/20 ,  A23D9/04

CPC classification number: C12N9/20 ,  C12Y301/01003 ,  A23D9/04

Abstract: The present invention relates to a polypeptide having lipase activity wherein the polypeptide when aligned with the polypeptide according to SEQ ID NO: 1, comprises at least an amino acid substitution G414X, wherein the numbering of amino acid position(s) is/are defined with reference to SEQ ID NO: 1. The invention further relates to a process for preparing a product comprising an oil or fat comprising bringing an intermediary form of the product comprising oil or fat into contact with a polypeptide as disclosed herein and the use of a polypeptide as disclosed herein to saturated fatty acids in an oil or fat.

公开(公告)号：US20230080079A1

公开(公告)日：2023-03-16

申请号：US17056200

申请日：2019-05-17

Applicant: DSM IP Assets B.V.

Inventor： René Marcel DE JONG ,  Willem BIJLEVELD ,  Paul Frederick MUGFORD

IPC: C12P7/6418 ,  C12N9/20 ,  A23L33/13 ,  A23L33/18 ,  A23L33/115 ,  A23K20/153 ,  A23K20/158 ,  A23K20/147

Abstract: The present invention relates to a polypeptide having lipase activity wherein the polypeptide when aligned with the polypeptide according to SEQ ID NO: 1, comprises at least an amino acid substitution L410X and optionally one or more amino acid substitutions chosen from S365Q, S365N, L413M, G414A, G414S, G414V, G414T, V534L and V534l, wherein the 10 numbering of amino acid position(s) is/are defined with reference to SEQ ID NO: 1. The invention further relates to a process for preparing a product comprising an oil or fat comprising bringing an intermediary form of the product comprising oil or fat into contact with a polypeptide as disclosed herein and the use of a polypeptide as disclosed herein to saturated fatty acids in an oil or fat.

公开(公告)号：US20210238499A1

公开(公告)日：2021-08-05

申请号：US16972153

申请日：2019-06-04

Applicant: DSM IP Assets B.V.

Inventor： Jaroslav A. KRALOVEC ,  Paul Frederick MUGFORD ,  Alfred ROLLE

IPC: C11C3/02

Abstract: A method for producing diglycerides is provided. The method includes combining (i) an oil comprising at least one polyunsaturated fatty acid in the form of ethyl esters, free fatty acids, and/or combinations thereof, (ii) lipase, and (iii) glycerol in water to produce diglycerides with a high level of purity. Highly pure diglycerides obtained according to the method are also provided.