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    MUTANT LIPASE AND USE THEREOF
    2.
    发明公开
    MUTANT LIPASE AND USE THEREOF 审中-公开

    公开(公告)号：US20230250406A1

    公开(公告)日：2023-08-10

    申请号：US17056230

    申请日：2019-05-17

    Applicant: DSM IP Assets B.V.

    Inventor： René Marcel DE JONG ,  Willem BIJLEVELD ,  Paul Frederick MUGFORD

    IPC: C12N9/20 ,  A23D9/04

    CPC classification number: C12N9/20 ,  C12Y301/01003 ,  A23D9/04

    Abstract: The present invention relates to a polypeptide having lipase activity wherein the polypeptide when aligned with the polypeptide according to SEQ ID NO: 1, comprises at least an amino acid substitution G414X, wherein the numbering of amino acid position(s) is/are defined with reference to SEQ ID NO: 1. The invention further relates to a process for preparing a product comprising an oil or fat comprising bringing an intermediary form of the product comprising oil or fat into contact with a polypeptide as disclosed herein and the use of a polypeptide as disclosed herein to saturated fatty acids in an oil or fat.

    MUTANT LIPASE AND USE THEREOF
    3.
    发明申请
    MUTANT LIPASE AND USE THEREOF 有权

    公开(公告)号：US20230080079A1

    公开(公告)日：2023-03-16

    申请号：US17056200

    申请日：2019-05-17

    Applicant: DSM IP Assets B.V.

    Inventor： René Marcel DE JONG ,  Willem BIJLEVELD ,  Paul Frederick MUGFORD

    IPC: C12P7/6418 ,  C12N9/20 ,  A23L33/13 ,  A23L33/18 ,  A23L33/115 ,  A23K20/153 ,  A23K20/158 ,  A23K20/147

    Abstract: The present invention relates to a polypeptide having lipase activity wherein the polypeptide when aligned with the polypeptide according to SEQ ID NO: 1, comprises at least an amino acid substitution L410X and optionally one or more amino acid substitutions chosen from S365Q, S365N, L413M, G414A, G414S, G414V, G414T, V534L and V534l, wherein the 10 numbering of amino acid position(s) is/are defined with reference to SEQ ID NO: 1. The invention further relates to a process for preparing a product comprising an oil or fat comprising bringing an intermediary form of the product comprising oil or fat into contact with a polypeptide as disclosed herein and the use of a polypeptide as disclosed herein to saturated fatty acids in an oil or fat.

    PROCESS FOR ENZYMATIC OIL DEGUMMING
    4.
    发明申请
    PROCESS FOR ENZYMATIC OIL DEGUMMING 有权

    公开(公告)号：US20210371770A1

    公开(公告)日：2021-12-02

    申请号：US17052907

    申请日：2019-05-06

    Applicant: DSM IP ASSETS B.V.

    Inventor： Ying ZHA ,  Arjen SEIN ,  Willem BIJLEVELD

    IPC: C11B3/00

    Abstract: The present invention relates to a process for reducing an amount of intact phospholipids in a triacylglyceride oil comprising incubating the oil with a polypeptide having phospholipase A1 activity, wherein the polypeptide comprises a polypeptide having at least 80% identity to the mature amino acid sequence of SEQ ID NO: 1.

    PHOSPHOLIPASE C
    5.
    发明申请
    PHOSPHOLIPASE C 审中-公开

    公开(公告)号：US20180134986A1

    公开(公告)日：2018-05-17

    申请号：US15564275

    申请日：2016-04-08

    Applicant: DSM IP ASSETS B.V.

    Inventor： Arjen SEIN ,  Rene Marcel DE JONG ,  Evert Tjeerd VAN RIJ ,  Willem BIJLEVELD ,  Maarten Hotse WILBRINK

    IPC: C11B3/00 ,  C12N9/16 ,  C12P7/64

    CPC classification number: C11B3/003 ,  C12N9/16 ,  C12P7/20 ,  C12P7/64 ,  C12P7/6445 ,  C12Y301/04003

    Abstract: The present invention relates to a polypeptide having phospholipase C activity, selected from the group consisting of i. a polypeptide comprising a mature polypeptide sequence of SEQ ID NO: 2; ii. a polypeptide that has least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% identity to the mature polypeptide sequence of SEQ ID NO: 2; iii. a polypeptide encoded by a nucleic acid that hybridizes under medium stringency, preferably under high stringency conditions to the complementary strand of the mature polypeptide coding sequence of SEQ ID NO:1; iv. a polypeptide encoded by a nucleic acid that has at least 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or 100% identity to the mature polypeptide coding sequence of SEQ ID NO: 1. A process for degumming a vegetable oil comprising contacting a vegetable oil comprising phospholipids with a polypeptide having phospholipase C activity of the invention or a composition of the invention, wherein phospholipids are hydrolysed into diacylglycerol and phosphate ester and/or phosphate, separating the phosphate ester and/or phosphate from the vegetable oil wherein a degummed vegetable oil is obtained.

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