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公开(公告)号：US20060257895A1
公开(公告)日：2006-11-16
申请号：US11361316
申请日：2006-02-24
申请人： Daniel Pinkel , Joe Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
发明人： Daniel Pinkel , Joe Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , C12Q2600/156 , G06F15/025 , Y10S436/813 , C12Q2545/114 , C12Q2537/157
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
摘要翻译： 公开了包括使用原位杂交来检测一个或多个基因组中的异常核酸序列拷贝数的新方法，其中结合参考染色体扩增的多个基因座的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交（CGH）的发明提供了确定一个或多个受试者基因组或其部分（例如肿瘤细胞）中核酸序列的相对拷贝数的方法，作为这些序列的位置的函数 参考基因组（例如，正常人类基因组）。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异，以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增，重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。
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公开(公告)号：US06335167B1
公开(公告)日：2002-01-01
申请号：US09311835
申请日：1999-05-14
申请人： Daniel Pinkel , Joe W. Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
发明人： Daniel Pinkel , Joe W. Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
IPC分类号： C12Q168
CPC分类号： C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , C12Q2600/156 , G06F15/025 , Y10S436/813 , C12Q2545/114 , C12Q2537/157
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
摘要翻译： 公开了包括使用原位杂交来检测一个或多个基因组中的异常核酸序列拷贝数的新方法，其中结合参考染色体扩增的多个基因座的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交（CGH）的发明提供了确定一个或多个受试者基因组或其部分（例如肿瘤细胞）中核酸序列的相对拷贝数的方法，作为这些序列的位置的函数 参考基因组（例如，正常人类基因组）。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异，以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增，重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。
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公开(公告)号：US08021837B2
公开(公告)日：2011-09-20
申请号：US11361316
申请日：2006-02-24
申请人： Daniel Pinkel , Joe W Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
发明人： Daniel Pinkel , Joe W Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
IPC分类号： C12Q1/68 , G01N33/566 , G01N33/53 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , C12Q2600/156 , G06F15/025 , Y10S436/813 , C12Q2545/114 , C12Q2537/157
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
摘要翻译： 公开了包括使用原位杂交来检测一个或多个基因组中的异常核酸序列拷贝数的新方法，其中结合参考染色体扩增的多个基因座的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交（CGH）的发明提供了确定一个或多个受试者基因组或其部分（例如肿瘤细胞）中核酸序列的相对拷贝数的方法，作为这些序列的位置的函数 参考基因组（例如，正常人类基因组）。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异，以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增，重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。
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公开(公告)号：US07094534B2
公开(公告)日：2006-08-22
申请号：US09912818
申请日：2001-07-24
申请人： Daniel Pinkel , Joe W. Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
发明人： Daniel Pinkel , Joe W. Gray , Anne Kallioniemi , Ollie-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
CPC分类号： C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , C12Q2600/156 , G06F15/025 , Y10S436/813 , C12Q2545/114 , C12Q2537/157
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
摘要翻译： 公开了包括使用原位杂交来检测一个或多个基因组中的异常核酸序列拷贝数的新方法，其中结合参考染色体扩增的多个基因座的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交（CGH）的发明提供了确定一个或多个受试者基因组或其部分（例如肿瘤细胞）中核酸序列的相对拷贝数的方法，作为这些序列的位置的函数 参考基因组（例如，正常人类基因组）。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异，以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增，重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。
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公开(公告)号：US20050118634A1
公开(公告)日：2005-06-02
申请号：US11017493
申请日：2004-12-17
CPC分类号： C12Q1/6841 , C12Q1/6809 , C12Q1/6853 , C12Q1/6886 , C12Q2600/158 , C12Q2545/114 , C12Q2545/113 , C12Q2537/143 , C12Q2525/151 , C12Q2563/107
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
摘要翻译： 公开了包括使用原位杂交来检测一个或多个基因组中的异常核酸序列拷贝数的新方法，其中结合参考染色体扩增的多个基因座的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交（CGH）的发明提供了确定一个或多个受试者基因组或其部分（例如肿瘤细胞）中核酸序列的相对拷贝数的方法，作为这些序列的位置的函数 参考基因组（例如，正常人类基因组）。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异，以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增，重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。
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公开(公告)号：US5965362A
公开(公告)日：1999-10-12
申请号：US562965
申请日：1995-11-27
申请人： Daniel Pinkel , Joe W. Gray , Anne Kallioniemi , Olli-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
发明人： Daniel Pinkel , Joe W. Gray , Anne Kallioniemi , Olli-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
CPC分类号： C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , G06F15/025 , C12Q2600/156 , Y10S436/813
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
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公开(公告)号：US20060063168A1
公开(公告)日：2006-03-23
申请号：US11060644
申请日：2005-02-16
申请人： Donna Albertson , Daniel Pinkel , Jane Fridyland , Bing Huey , Antoine Snijders , Joe Gray , Anne Kallioniemi , Olli-Pekka Kallioniemi , Frederic Waldman
发明人： Donna Albertson , Daniel Pinkel , Jane Fridyland , Bing Huey , Antoine Snijders , Joe Gray , Anne Kallioniemi , Olli-Pekka Kallioniemi , Frederic Waldman
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q2600/156 , C12Q2565/601
摘要： The present invention provides a method of detecting nucleotide sequence differences between two nucleic acid samples. The method employs a comparative genomic hybridization (CGH) technique to analyze the sequence differences between the samples. This method permits the identification of small sequence differences (e.g., sequence divergence of 1% or less) in nucleic acid samples of high complexity (e.g., an entire genome).
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公开(公告)号：US5856097A
公开(公告)日：1999-01-05
申请号：US562898
申请日：1995-11-27
申请人： Daniel Pinkel , Joe W. Gray , Anne Kallioniemi , Olli-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
发明人： Daniel Pinkel , Joe W. Gray , Anne Kallioniemi , Olli-Pekka Kallioniemi , Frederic Waldman , Masaru Sakamoto
CPC分类号： C12Q1/6809 , C12Q1/6841 , C12Q1/6886 , G06F15/025 , C12Q2600/156 , Y10S436/813
摘要： Disclosed are new methods comprising the use, of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
摘要翻译： 公开了包括使用原位杂交以检测一个或多个基因组中的异常核酸序列拷贝数的新方法，其中与参考染色体扩增中的多个基因座结合的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交（CGH）的发明提供了确定一个或多个受试者基因组或其部分（例如肿瘤细胞）中核酸序列的相对拷贝数的方法，作为这些序列的位置的函数 参考基因组（例如，正常人类基因组）。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异，以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增，重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。
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公开(公告)号：US20060292608A1
公开(公告)日：2006-12-28
申请号：US11431094
申请日：2006-05-08
CPC分类号： C12Q1/6841 , C12Q1/6809 , C12Q1/6853 , C12Q1/6886 , C12Q2600/158 , C12Q2545/114 , C12Q2545/113 , C12Q2537/143 , C12Q2525/151 , C12Q2563/107
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
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公开(公告)号：US5665549A
公开(公告)日：1997-09-09
申请号：US466122
申请日：1995-06-06
CPC分类号： C12Q1/6841 , C12Q1/6809 , C12Q1/6853 , C12Q1/6886 , C12Q2600/158
摘要： Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread. Amplifications, duplications and/or deletions in the subject genome(s) can be detected. Also provided is a method of determining the absolute copy numbers of substantially all RNA or DNA sequences in subject cell(s) or cell population(s).
摘要翻译： 公开了包括使用原位杂交来检测一个或多个基因组中的异常核酸序列拷贝数的新方法，其中结合参考染色体扩增的多个基因座的重复序列基本上被去除和/或其杂交信号被抑制。 称为比较基因组杂交（CGH）的发明提供了确定一个或多个受试者基因组或其部分（例如肿瘤细胞）中核酸序列的相对拷贝数的方法，作为这些序列的位置的函数 参考基因组（例如，正常人类基因组）。 比较来自每个标记的对象核酸的信号的强度和/或来自标记的目标核酸序列的不同信号之间的比率差异，以确定一个或多个核酸序列中核酸序列的相对拷贝数 主题基因组作为沿着参考染色体扩散的位置的函数。 可以检测主题基因组中的扩增，重复和/或缺失。 还提供了确定受试细胞或细胞群体中基本上所有RNA或DNA序列的绝对拷贝数的方法。
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