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11 条记录 (耗时 0.019 秒)

    Wide field image for quantitative analysis of microarrays
    2.
    发明申请
    Wide field image for quantitative analysis of microarrays 有权
    用于定量分析微阵列的宽场图像

    公开(公告)号：US20050260741A1

    公开(公告)日：2005-11-24

    申请号：US10850986

    申请日：2004-05-20

    申请人： Donna Albertson ,  Daniel Pinkel

    发明人： Donna Albertson ,  Daniel Pinkel

    IPC分类号： C12M1/34 ,  C12Q1/68 ,  H04N7/18 ,  H04N9/47

    CPC分类号： G02B21/365 ,  C12M41/36 ,  G01N21/6452 ,  G01N2021/6463 ,  G02B21/08 ,  G02B27/0018 ,  G02B2207/113

    摘要： This invention provides an imaging system for high-accuracy quantitative analysis of a microarray. In certain embodiments, the system comprises a broad band excitation light source that provides Kohler illumination of said microarray at an incident angle that ranges from about 30 degrees to about 75 degrees from the normal to the microarray, and that has less than about ±25 percent variation in intensity over the array at all wavelengths ranging from 400 to 800 nm; a support for holding a microarray; a detection lens system that is chromatically corrected so the apparent position of the microarray or a feature comprising the microarray varies by less than 10 μm as the detection wavelength varies from about 400 to about 800 nm; and a detection device for detecting and optionally recording an image produced by said detection lens system.

    摘要翻译： 本发明提供了一种用于微阵列的高精度定量分析的成像系统。 在某些实施方案中，该系统包括宽带激发光源，其以距离微阵列法线约30度至约75度的入射角提供所述微阵列的科勒照明，并且具有小于约±25％ 在400至800 nm范围内的所有波长阵列上的强度变化; 支持持有微阵列; 检测透镜系统被校正为使得当检测波长从约400至约800nm变化时，微阵列的表观位置或包含微阵列的特征变化小于10um; 以及用于检测和可选地记录由所述检测透镜系统产生的图像的检测装置。

    Comparative fluorescence hybridization to nucleic acid arrays
    5.
    发明授权
    Comparative fluorescence hybridization to nucleic acid arrays 有权
    与核酸阵列的比较荧光杂交

    公开(公告)号：US07776536B2

    公开(公告)日：2010-08-17

    申请号：US11727935

    申请日：2007-03-29

    申请人： Daniel Pinkel ,  Donna Albertson ,  Joe W. Gray

    发明人： Daniel Pinkel ,  Donna Albertson ,  Joe W. Gray

    IPC分类号： C12Q1/68 ,  G01N21/76 ,  G01N33/50 ,  C07H21/02 ,  C07H21/04

    CPC分类号： C12Q1/6816 ,  C12Q1/6837 ,  G06F19/20 ,  C12Q2545/101

    摘要： The present invention provides methods of determining relative copy number of target nucleic acids and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acids immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acids are hybridized. The hybridization of the labeled nucleic acids to the solid surface is then detected using standard techniques.

    摘要翻译： 本发明提供了确定靶核酸的相对拷贝数和与疾病相关的染色体异常的精确映射的方法。 本发明的方法使用固定在固体表面上的靶核酸，其中包含两组差异标记的核酸的样品与之杂交。 然后使用标准技术检测标记的核酸与固体表面的杂交。

    Comparative fluorescence hybridization to nucleic acid arrays
    6.
    发明授权
    Comparative fluorescence hybridization to nucleic acid arrays 失效
    与核酸阵列的比较荧光杂交

    公开(公告)号：US06562565B1

    公开(公告)日：2003-05-13

    申请号：US08670953

    申请日：1996-06-26

    申请人： Daniel Pinkel ,  Donna Albertson ,  Joe W. Gray

    发明人： Daniel Pinkel ,  Donna Albertson ,  Joe W. Gray

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6816 ,  C12Q1/6837 ,  G06F19/20 ,  C12Q2545/101

    摘要： The present invention provides methods of determining relative copy number of target nucleic acids and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acids immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acids are hybridized. The hybridization of the labeled nucleic acids to the solid surface is then detected using standard techniques.

    摘要翻译： 本发明提供了确定靶核酸的相对拷贝数和与疾病相关的染色体异常的精确映射的方法。 本发明的方法使用固定在固体表面上的靶核酸，其中包含两组差异标记的核酸的样品与之杂交。 然后使用标准技术检测标记的核酸与固体表面的杂交。

    Specimen illumination apparatus with optical cavity for dark field illumination
    7.
    发明授权
    Specimen illumination apparatus with optical cavity for dark field illumination 失效
    具有用于暗场照明的光学腔的样品照明装置

    公开(公告)号：US5982534A

    公开(公告)日：1999-11-09

    申请号：US878145

    申请日：1997-06-18

    申请人： Daniel Pinkel ,  Damir Sudar ,  Donna Albertson

    发明人： Daniel Pinkel ,  Damir Sudar ,  Donna Albertson

    IPC分类号： G01N21/64 ,  G02B6/12 ,  G02B21/06 ,  G02B21/10 ,  G02B21/16 ,  G02B21/34 ,  C07H21/04 ,  C12Q1/68 ,  G01N21/00

    CPC分类号： G02B21/10 ,  G01N21/6458 ,  G01N2021/8822 ,  G01N21/8806 ,  G02B2006/12114

    摘要： An illumination apparatus with a specimen slide holder, an illumination source, an optical cavity producing multiple reflection of illumination light to a specimen comprising a first and a second reflective surface arranged to achieve multiple reflections of light to a specimen is provided. The apparatus can further include additional reflective surfaces to achieve the optical cavity, a slide for mounting the specimen, a coverslip which is a reflective component of the optical cavity, one or more prisms for directing light within the optical cavity, antifading solutions for improving the viewing properties of the specimen, an array of materials for analysis, fluorescent components, curved reflective surfaces as components of the optical cavity, specimen detection apparatus, optical detection equipment, computers for analysis of optical images, a plane polarizer, fiberoptics, light transmission apertures, microscopic components, lenses for viewing the specimen, and upper and lower mirrors above and below the specimen slide as components of the optical cavity. Methods of using the apparatus are also provided.

    摘要翻译： 提供一种照明装置，其具有试样滑动架，照明源，向试样产生多次反射照明光的光学腔，该样本包括布置成实现对样本的多次反射的第一和第二反射表面。 该装置还可以包括附加的反射表面以实现光学腔，用于安装样本的滑块，作为光学腔的反射部件的盖玻片，用于将光引导到光腔内的一个或多个棱镜，用于改善 样品的观察特性，用于分析的材料阵列，荧光组分，作为光学腔的组分的弯曲反射表面，样品检测装置，光学检测设备，用于光学图像分析的计算机，平面偏振器，光纤，光传输孔 ，微观组件，用于观察样品的透镜，以及作为光学腔的部件的样品滑动件上方和下方的上镜和下镜。 还提供了使用该装置的方法。

    Comparative fluorescence hybridization to nucleic acid arrays
    9.
    发明申请
    Comparative fluorescence hybridization to nucleic acid arrays 有权
    与核酸阵列的比较荧光杂交

    公开(公告)号：US20070172883A1

    公开(公告)日：2007-07-26

    申请号：US11727935

    申请日：2007-03-29

    申请人： Daniel Pinkel ,  Donna Albertson ,  Joe Gray

    发明人： Daniel Pinkel ,  Donna Albertson ,  Joe Gray

    IPC分类号： C12Q1/68 ,  G06F19/00

    CPC分类号： C12Q1/6816 ,  C12Q1/6837 ,  G06F19/20 ,  C12Q2545/101

    摘要： The present invention provides methods of determining relative copy number of target nucleic acids and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acids immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acids are hybridized. The hybridization of the labeled nucleic acids to the solid surface is then detected using standard techniques.

    摘要翻译： 本发明提供了确定靶核酸的相对拷贝数和与疾病相关的染色体异常的精确映射的方法。 本发明的方法使用固定在固体表面上的靶核酸，其中包含两组差异标记的核酸的样品与之杂交。 然后使用标准技术检测标记的核酸与固体表面的杂交。

    Capillary pins for high-efficiency microarray printing device
    10.
    发明申请
    Capillary pins for high-efficiency microarray printing device 有权
    用于高效微阵列打印设备的毛细管引脚

    公开(公告)号：US20050169808A1

    公开(公告)日：2005-08-04

    申请号：US11017625

    申请日：2004-12-15

    申请人： Daniel Pinkel ,  Donna Albertson ,  Greg Hamilton ,  Nils Brown ,  Robert Nordmeyer

    发明人： Daniel Pinkel ,  Donna Albertson ,  Greg Hamilton ,  Nils Brown ,  Robert Nordmeyer

    IPC分类号： B41J2/005 ,  B41J2/25 ,  C12Q1/68 ,  B01L3/02 ,  B41J2/01

    CPC分类号： B41J2/25 ,  B41J2/005 ,  C12Q1/6837 ,  C12Q2565/507

    摘要： This invention provides improved components (e.g. array “pins”, print head, substrate platen, print head platen, and the like) for microarray printing devices as well as microarray printing devices incorporating such components. In one embodiment, this invention provides a microarray print head comprising a plurality of glass or quartz spotting capillaries disposed in a support that maintains a fixed spacing between the spotting capillaries and that permits the spotting capillaries to move in a direction parallel to the long axis of the capillaries.

    摘要翻译： 本发明提供了用于微阵列打印装置的改进的组件（例如，阵列“针”，打印头，基板压板，打印头压板等）以及并入这些组件的微阵列打印装置。 在一个实施方案中，本发明提供了一种微阵列打印头，其包括设置在支撑体中的多个玻璃或石英斑点毛细管，其在斑点毛细管之间保持固定的间隔，并且允许斑点毛细管沿平行于长轴的方向移动 毛细血管