公开(公告)号：US5677153A

公开(公告)日：1997-10-14

申请号：US346333

申请日：1994-11-29

Applicant: David Botstein ,  Timothy Palzkill

Inventor： David Botstein ,  Timothy Palzkill

IPC: C12N15/10 ,  C12N15/64

CPC classification number: C12N15/102

Abstract: The invention relates to methods and mutation linkers to modify DNA, to methods for producing libraries containing a multiplicity of modified DNA, and to methods for using such libraries for screening modified proteins encoded by such DNA. The DNA targeted for modification typically encodes a polypeptide such as an enzyme. The libraries are used to determine the effect of such modification or the interaction of the modified polypeptides with a target. In preferred embodiments, the invention relates to methods for making and using libraries containing DNA encoding modified antibiotic hydrolases to screen antibiotics against one or more of the modified antibiotic hydrolases produced by such libraries. Susceptibility or lack of susceptibility of an antibiotic to neutralization provides an indication of whether wild-type antibiotic hydrolases are likely to mutate to confer resistance to the antibiotic.

Abstract translation: 本发明涉及用于修饰DNA的方法和突变接头，用于产生含有多个修饰的DNA的文库的方法，以及使用这种文库筛选由此DNA编码的修饰蛋白的方法。 靶向修饰的DNA通常编码多肽，例如酶。 文库用于确定这种修饰的作用或修饰的多肽与靶标的相互作用。 在优选的实施方案中，本发明涉及制备和使用含有编码经修饰的抗生素水解酶的DNA的文库的方法，以筛选针对一种或多种由这些文库产生的经修饰的抗生素水解酶的抗生素。 敏感性或抗生素对中和的敏感性不足提供了野生型抗生素水解酶是否可能发生变异以赋予抗生素抗性的指征。

公开(公告)号：US20120329842A1

公开(公告)日：2012-12-27

申请号：US13332793

申请日：2011-12-21

Applicant: Yongcheng Song ,  Timothy Palzkill ,  Pinhong Chen ,  Lori Horton

Inventor： Yongcheng Song ,  Timothy Palzkill ,  Pinhong Chen ,  Lori Horton

IPC: A61K31/426 ,  C07D277/56 ,  A61P31/04 ,  C12N9/99

CPC classification number: A61K31/425

Abstract: The present invention concerns methods and/or compositions for treatment and/or prevention of bacterial infection wherein the bacteria has at least one metallo-β-lactamase. The bacteria are provided with an inhibitor of the metallo-β-lactamase, for example in conjunction with an antibiotic that targets the bacteria. The bacteria may be a drug-resistant strain or susceptible to becoming a drug-resistant strain. In specific embodiments, the bacteria is Pseudomonas or Acinetobacter spp.

Abstract translation: 本发明涉及用于治疗和/或预防细菌感染的方法和/或组合物，其中细菌具有至少一种金属 - β-内酰胺酶。 细菌具有金属 - β-内酰胺酶的抑制剂，例如与针对细菌的抗生素结合。 细菌可能是耐药菌株或易成为耐药菌株。 在具体实施方案中，细菌是假单胞菌属或不动杆菌属。

公开(公告)号：US20110020786A1

公开(公告)日：2011-01-27

申请号：US12681835

申请日：2008-10-07

Applicant: Timothy Palzkill ,  Zanna Beharry

Inventor： Timothy Palzkill ,  Zanna Beharry

IPC: C12Q1/70 ,  C07K2/00 ,  C12M1/34

CPC classification number: A61K38/10 ,  C07K7/08 ,  G01N33/56983 ,  G01N2333/08

Abstract: Noroviruses are recognized as the most common cause of outbreaks of acute gastroenteritis in humans. Therefore, the present invention relates to peptides or dendrimers that bind Noroviruses and the methods for identifying and synthesizing these peptides. It also relates to the detection of Noroviruses using said peptides or dendrimers formed by them.

Abstract translation: 诺维病毒被认为是人类急性胃肠炎爆发的最常见原因。 因此，本发明涉及结合诺如病毒的肽或树状大分子以及鉴定和合成这些肽的方法。 它还涉及使用由它们形成的所述肽或树枝状大分子的诺如病毒的检测。

公开(公告)号：US20050186197A1

公开(公告)日：2005-08-25

申请号：US11059226

申请日：2005-02-16

Applicant: Timothy Palzkill ,  Wanzhi Huang

Inventor： Timothy Palzkill ,  Wanzhi Huang

IPC: A61K38/00 ,  A61K38/54 ,  C07D20060101 ,  C07K7/06 ,  C12N9/99

CPC classification number: C07K7/06

Abstract: Peptide inhibitors of β-lactamases have been identified by the synthesis of peptide arrays using synthesis SPOT technology. These peptide inhibitors of β-lactamase have activity against a broad spectrum of β-lactamases and are useful in a variety of applications.

Abstract translation: 已经通过使用合成SPOT技术合成肽阵列来鉴定β-内酰胺酶的肽抑制剂。 β-内酰胺酶的这些肽抑制剂具有抗广泛β-内酰胺酶的活性，并且可用于各种应用。

公开(公告)号：US20120021405A1

公开(公告)日：2012-01-26

申请号：US13146155

申请日：2010-01-26

Applicant: Timothy Palzkill ,  Wanzhi Huang ,  Mary K. Estes

Inventor： Timothy Palzkill ,  Wanzhi Huang ,  Mary K. Estes

IPC: C12Q1/70 ,  C12N1/19 ,  C12N5/10 ,  C12N1/21 ,  C07K16/10 ,  C12N15/63

CPC classification number: C07K16/10 ,  C07K2317/622

Abstract: The present invention concerns compositions and methods for detecting Norovirus or Norovirus particles. In particular, the present invention encompasses antibodies for detecting Norovirus or Norovirus particles, including, for example, monoclonal antibodies that have broad specificity of binding to various genogroups of norovirus.

Abstract translation: 本发明涉及用于检测诺如病毒或诺如病毒颗粒的组合物和方法。 特别地，本发明包括用于检测诺如病毒或诺如病毒颗粒的抗体，包括例如具有与诺如病毒的各种基因组结合的广泛特异性的单克隆抗体。