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    Methods for modifying DNA and for detecting effects of such modification on interaction of encoded modified polypeptides with target substrates
    1.
    发明授权
    Methods for modifying DNA and for detecting effects of such modification on interaction of encoded modified polypeptides with target substrates 失效
    用于修饰DNA和用于检测这种修饰对编码的修饰多肽与靶基质相互作用的方法

    公开(公告)号：US5677153A

    公开(公告)日：1997-10-14

    申请号：US346333

    申请日：1994-11-29

    Applicant: David Botstein ,  Timothy Palzkill

    Inventor： David Botstein ,  Timothy Palzkill

    IPC: C12N15/10 ,  C12N15/64

    CPC classification number: C12N15/102

    Abstract: The invention relates to methods and mutation linkers to modify DNA, to methods for producing libraries containing a multiplicity of modified DNA, and to methods for using such libraries for screening modified proteins encoded by such DNA. The DNA targeted for modification typically encodes a polypeptide such as an enzyme. The libraries are used to determine the effect of such modification or the interaction of the modified polypeptides with a target. In preferred embodiments, the invention relates to methods for making and using libraries containing DNA encoding modified antibiotic hydrolases to screen antibiotics against one or more of the modified antibiotic hydrolases produced by such libraries. Susceptibility or lack of susceptibility of an antibiotic to neutralization provides an indication of whether wild-type antibiotic hydrolases are likely to mutate to confer resistance to the antibiotic.

    Abstract translation: 本发明涉及用于修饰DNA的方法和突变接头，用于产生含有多个修饰的DNA的文库的方法，以及使用这种文库筛选由此DNA编码的修饰蛋白的方法。 靶向修饰的DNA通常编码多肽，例如酶。 文库用于确定这种修饰的作用或修饰的多肽与靶标的相互作用。 在优选的实施方案中，本发明涉及制备和使用含有编码经修饰的抗生素水解酶的DNA的文库的方法，以筛选针对一种或多种由这些文库产生的经修饰的抗生素水解酶的抗生素。 敏感性或抗生素对中和的敏感性不足提供了野生型抗生素水解酶是否可能发生变异以赋予抗生素抗性的指征。

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