公开(公告)号：US07199227B2

公开(公告)日：2007-04-03

申请号：US10172094

申请日：2002-06-14

申请人： Donald G. Jackson ,  Matthew V. Lorenzi ,  Ricardo M. Attar ,  Marco Gottardis ,  Liana M. Lee ,  John N. Feder

发明人： Donald G. Jackson ,  Matthew V. Lorenzi ,  Ricardo M. Attar ,  Marco Gottardis ,  Liana M. Lee ,  John N. Feder

IPC分类号： C07H21/04

CPC分类号： C12N9/16

摘要： The present invention relates to newly discovered human histone deacetylases (HDACs), also referred to as histone deacetylase-like polypeptides. The polynucleotide sequences and encoded polypeptides of the novel HDACs are encompassed by the invention, as well as vectors comprising these polynucleotides and host cells comprising these vectors. The invention also relates to antibodies that bind to the disclosed HDAC polypeptides, and methods employing these antibodies. Also related are methods of screening for modulators, such as inhibitors or antagonists, or agonists. The invention also relates to diagnostic and therapeutic applications which employ the disclosed HDAC polynucleotides, polypeptides, and antibodies, and HDAC modulators. Such applications can be used with diseases and disorders associated with abnormal cell growth or proliferation, cell differentiation, and cell survival, e.g., neoplastic cell growth, and especially breast and prostate cancers or tumors.

摘要翻译： 本发明涉及新发现的人类组蛋白脱乙酰酶（HDAC），也称为组蛋白脱乙酰酶样多肽。 本发明涵盖了新型HDAC的多核苷酸序列和编码多肽，以及包含这些多核苷酸的载体和包含这些载体的宿主细胞。 本发明还涉及结合所公开的HDAC多肽的抗体以及使用这些抗体的方法。 还涉及筛选调节剂的方法，例如抑制剂或拮抗剂或激动剂。 本发明还涉及使用所公开的HDAC多核苷酸，多肽和抗体以及HDAC调节剂的诊断和治疗应用。 这种应用可以用于与异常细胞生长或增殖，细胞分化和细胞存活相关的疾病和病症，例如肿瘤细胞生长，特别是乳腺癌和前列腺癌或肿瘤。

公开(公告)号：US07521044B2

公开(公告)日：2009-04-21

申请号：US11497205

申请日：2006-08-01

申请人： Donald G. Jackson ,  Matthew V. Lorenzi ,  Liana M. Lee ,  John N. Feder

发明人： Donald G. Jackson ,  Matthew V. Lorenzi ,  Liana M. Lee ,  John N. Feder

IPC分类号： A61K38/43 ,  C12N9/00 ,  C07K14/00

CPC分类号： C12N9/16

摘要： The present invention relates to newly discovered human histone deacetylases (HDACs), also referred to as histone deacetylase-like polypeptides. The polynucleotide sequences and encoded polypeptides of the novel HDACs are encompassed by the invention, as well as vectors comprising these polynucleotides and host cells comprising these vectors. The invention also relates to antibodies that bind to the disclosed HDAC polypeptides, and methods employing these antibodies. Also related are methods of screening for modulators, such as inhibitors or antagonists, or agonists. The invention also relates to diagnostic and therapeutic applications which employ the disclosed HDAC polynucleotides, polypeptides, and antibodies, and HDAC modulators. Such applications can be used with diseases and disorders associated with abnormal cell growth or proliferation, cell differentiation, and cell survival, e.g., neoplastic cell growth, and especially breast and prostate cancers or tumors.

摘要翻译： 本发明涉及新发现的人类组蛋白脱乙酰酶（HDAC），也称为组蛋白脱乙酰酶样多肽。 本发明涵盖了新型HDAC的多核苷酸序列和编码多肽，以及包含这些多核苷酸的载体和包含这些载体的宿主细胞。 本发明还涉及结合所公开的HDAC多肽的抗体以及使用这些抗体的方法。 还涉及筛选调节剂的方法，例如抑制剂或拮抗剂或激动剂。 本发明还涉及使用所公开的HDAC多核苷酸，多肽和抗体以及HDAC调节剂的诊断和治疗应用。 这种应用可以用于与异常细胞生长或增殖，细胞分化和细胞存活相关的疾病和病症，例如肿瘤细胞生长，特别是乳腺癌和前列腺癌或肿瘤。

公开(公告)号：US06323316B1

公开(公告)日：2001-11-27

申请号：US09202548

申请日：1998-12-15

申请人： Matthew V. Lorenzi ,  Toru Miki

发明人： Matthew V. Lorenzi ,  Toru Miki

IPC分类号： C07K1400

CPC分类号： C07K14/4702 ,  C07K14/71 ,  C07K2319/00

摘要： A novel gene designated as FRAG 1 and its encoded protein are disclosed. A fusion protein called FGFR2-ROS, which is formed by chromosomal rearrangement of rat FRAG1 with FGFR2, is also disclosed. Methods of producing FRAG1 protein, related fusion proteins, and antibodies against FRAG1 are disclosed, as are related pharmaceuticals and methods of using such nucleic acids, polypeptides, and antibodies.

摘要翻译： 公开了一种称为FRAG1及其编码蛋白的新基因。 还公开了通过大鼠FRAG1与FGFR2的染色体重排形成的称为FGFR2-ROS的融合蛋白。 公开了生产FRAG1蛋白，相关融合蛋白和针对FRAG1的抗体的方法，以及使用这种核酸，多肽和抗体的相关药物和方法。

公开(公告)号：US06608181B2

公开(公告)日：2003-08-19

申请号：US09942858

申请日：2001-08-29

申请人： Matthew V. Lorenzi ,  Toru Miki

发明人： Matthew V. Lorenzi ,  Toru Miki

IPC分类号： C07K1600

CPC分类号： C07K14/4702 ,  C07K14/71 ,  C07K2319/00

摘要： A novel gene designated as FRAG1 and its encoded protein is disclosed. A fusion protein called FGFR2-ROS, which is formed by chromosomal rearrangement of rat FRAG1 with FGFR2, is also disclosed. Methods of producing FRAG1 protein, related fusion proteins, and antibodies against FRAG1 are disclosed, as are related pharmaceuticals and methods of using such nucleic acids, polypeptides, and antibodies are also disclosed.

公开(公告)号：US07297493B2

公开(公告)日：2007-11-20

申请号：US10993191

申请日：2004-11-18

申请人： Matthew V. Lorenzi ,  Toru Miki

发明人： Matthew V. Lorenzi ,  Toru Miki

IPC分类号： C12Q1/68

CPC分类号： C07K14/4702 ,  C07K14/71 ,  C07K2319/00

摘要： A novel gene designated as FRAG1 from and its encoded protein is disclosed. A fusion protein called FGFR2-ROS, which is formed by chromosomal rearrangement of rat FRAG1 with FGFR2 is also disclosed. Methods of producing FRAG1 protein, related fusion proteins, and antibodies against FRAG1 are disclosed, as are related pharmaceuticals and methods of using such nucleic acids, polypeptides, and antibodies are also disclosed.

摘要翻译： 公开了一种称为FRAG1的新基因及其编码的蛋白质。 还公开了通过大鼠FRAG1与FGFR2的染色体重排形成的称为FGFR2-ROS的融合蛋白。 公开了生产FRAG1蛋白，相关融合蛋白和针对FRAG1的抗体的方法，并且还公开了相关药物和使用这些核酸，多肽和抗体的方法。

公开(公告)号：US07138246B2

公开(公告)日：2006-11-21

申请号：US11068606

申请日：2005-02-28

申请人： Matthew V. Lorenzi ,  Thomas Spires ,  Dan You ,  Roberto Weinmann ,  Marco Gottardis

发明人： Matthew V. Lorenzi ,  Thomas Spires ,  Dan You ,  Roberto Weinmann ,  Marco Gottardis

IPC分类号： G01N33/53 ,  G01N33/567

CPC分类号： G01N33/743

摘要： Methods for determining whether a test agent inhibits a 17β-HSD3, the methods comprising: obtaining a recombinant host cell that expresses the 17β-HSD3; obtaining a reaction mixture comprising the 17β-HSD3, androstenedione, and the test agent; measuring the amount of testosterone in the reaction mixture by a scintillation proximity assay (SPA) using SPA beads conjugated with a testosterone-specific antibody, wherein when the amount of testosterone is lower in the presence of a test agent than in the absence of the test agent, the test agent is identified as an inhibitor of the 17β-HSD3.

摘要翻译： 用于确定测试试剂是否抑制17β-HSD3的方法，所述方法包括：获得表达17β-HSD3的重组宿主细胞; 得到包含17β-HSD3，雄烯二酮和反应试剂的反应混合物; 通过使用与睾酮特异性抗体缀合的SPA珠的闪烁邻近测定（SPA）测量反应混合物中的睾酮的量，其中当在测试试剂存在下睾酮的量低于不存在测试时，睾酮的量较低 试剂被鉴定为17β-HSD3的抑制剂。

公开(公告)号：US06831170B2

公开(公告)日：2004-12-14

申请号：US10461180

申请日：2003-06-12

申请人： Matthew V. Lorenzi ,  Toru Miki

发明人： Matthew V. Lorenzi ,  Toru Miki

IPC分类号： C07H2104

CPC分类号： C07K14/4702 ,  C07K14/71 ,  C07K2319/00

摘要： A novel gene designated as FRAG1 from and its encoded protein is disclosed. A fusion protein called FGFR2-ROS, which is formed by chromosomal rearrangement of rat FRAG1 with FGFR2 is also disclosed. Methods of producing FRAG1 protein, related fusion proteins, and antibodies against FRAG1 are disclosed, as are related pharmaceuticals and methods of using such nucleic acids, polypeptides, and antibodies are also disclosed.

摘要翻译： 公开了一种称为FRAG1的新基因及其编码的蛋白质。 还公开了通过大鼠FRAG1与FGFR2的染色体重排形成的称为FGFR2-ROS的融合蛋白。 公开了生产FRAG1蛋白，相关融合蛋白和针对FRAG1的抗体的方法，并且还公开了相关药物和使用这些核酸，多肽和抗体的方法。