公开(公告)号：US10605739B2

公开(公告)日：2020-03-31

申请号：US13756570

申请日：2013-02-01

Applicant: EMD Millipore Corporation

Inventor： Gael Waiche

IPC: G01N21/64 ,  G01N21/76 ,  G01N33/58 ,  C12Q1/04

Abstract: The device for the microbiological analysis of a support adapted to contain microorganisms marked by a fluorophore agent, said agent being adapted to absorb light energy with an absorption spectrum (53) having a crest (53′) at a predetermined absorption wavelength (λ2) and to release that energy by emitting light with an emission spectrum (54) having a crest (54′) at a predetermined emission wavelength (λ3) distinct from said absorption wavelength (λ2), comprises illuminating means; the spectrum (55) of the excitation light coming from the illuminating means having a crest (55′) at a predetermined excitation wavelength (λ1) with a predetermined difference between the excitation (λ1) and absorption (λ2) wavelengths by virtue of which it is made easy to discriminate the light emitted by said fluorophore agent from that coming from the illuminating means.

公开(公告)号：US09732371B2

公开(公告)日：2017-08-15

申请号：US14036339

申请日：2013-09-25

Applicant: EMD Millipore Corporation

Inventor： Gael Waiche ,  Monika Kukuczka

IPC: C12Q1/24 ,  C12M1/30 ,  C12M1/26

CPC classification number: C12Q1/24 ,  C12M33/02 ,  C12M33/14

Abstract: A method for transferring a filter membrane (130) bearing a retentate to a reagent pad (310), in which the membrane is mounted in a support frame (120) having a shoulder (121) and a skirt (122) projecting from the shoulder, the pad being mounted on a cassette (300) of complementary form to said skirt, the skirt being adapted to slide sealingly on the cassette until it comes into abutment formed by the shoulder, the method comprising the steps of sealingly engaging the skirt of the membrane support frame on the cassette, establishing a pressure difference between the cassette and the membrane support frame such that the membrane adopts the shape of a dome pointing towards the pad, while maintaining the pressure difference, applying a force on the frame so as to make the skirt slide on the cassette until contact is established between the end of the dome and the pad, maintaining a force on the frame so as to move it downward until it comes into abutment formed by the shoulder while maintaining the pressure difference.

公开(公告)号：US20140017779A1

公开(公告)日：2014-01-16

申请号：US14036339

申请日：2013-09-25

Applicant: EMD Millipore Corporation

Inventor： Gael Waiche ,  Monika Kukuczka

IPC: C12Q1/24

CPC classification number: C12Q1/24 ,  C12M33/02 ,  C12M33/14

Abstract: A method for transferring a filter membrane (130) bearing a retentate to a reagent pad (310), in which the membrane is mounted in a support frame (120) having a shoulder (121) and a skirt (122) projecting from the shoulder, the pad being mounted on a cassette (300) of complementary form to said skirt, the skirt being adapted to slide sealingly on the cassette until it comes into abutment formed by the shoulder, the method comprising the steps of sealingly engaging the skirt of the membrane support frame on the cassette, establishing a pressure difference between the cassette and the membrane support frame such that the membrane adopts the shape of a dome pointing towards the pad, while maintaining the pressure difference, applying a force on the frame so as to make the skirt slide on the cassette until contact is established between the end of the dome and the pad, maintaining a force on the frame so as to move it downward until it comes into abutment formed by the shoulder while maintaining the pressure difference.

Abstract translation: 一种用于将带有滞留物的过滤膜（130）转移到试剂垫（310）的方法，其中所述膜安装在具有肩部（121）的支撑框架（120）和从肩部突出的裙部（122） ，所述垫被安装在所述裙部的互补形式的盒（300）上，所述裙部适于在所述盒子上密封地滑动，直到其进入与所述肩部形成的邻接，所述方法包括以下步骤：密封地接合所述裙部的裙部 膜盒支撑框架，在盒和膜支撑框架之间形成压力差，使得膜采取指向垫的圆顶形状，同时保持压力差，对框架施加力，以使 裙子在盒子上滑动，直到在圆顶和垫子的端部之间建立接触，保持框架上的力，以向下移动它，直到它与肩部形成邻接 压力差。

公开(公告)号：US20130143259A1

公开(公告)日：2013-06-06

申请号：US13756570

申请日：2013-02-01

Applicant: EMD Millipore Corporation

Inventor： Gael Waiche

IPC: G01N21/76

CPC classification number: G01N21/763 ,  C12Q1/04 ,  G01N21/6428 ,  G01N21/6447 ,  G01N21/645 ,  G01N33/582 ,  G01N2021/6419 ,  G01N2201/0627 ,  G01N2201/0634

Abstract: The device for the microbiological analysis of a support adapted to contain microorganisms marked by a fluorophore agent, said agent being adapted to absorb light energy with an absorption spectrum (53) having a crest (53′) at a predetermined absorption wavelength (λ2) and to release that energy by emitting light with an emission spectrum (54) having a crest (54′) at a predetermined emission wavelength (λ3) distinct from said absorption wavelength (λ2), comprises illuminating means; the spectrum (55) of the excitation light coming from the illuminating means having a crest (55′) at a predetermined excitation wavelength (λ1) with a predetermined difference between the excitation (λ1) and absorption (λ2) wavelengths by virtue of which it is made easy to discriminate the light emitted by said fluorophore agent from that coming from the illuminating means.

Abstract translation: 用于对适于含有由荧光团试剂标记的微生物的载体的微生物分析的装置，所述试剂适于用具有预定吸收波长（λ2）的波峰（53'）的吸收光谱（53）吸收光能，以及 通过发射具有不同于所述吸收波长（λ2）的预定发射波长（λ3）的峰（54'）的发射光谱（54）的光来释放该能量，包括照明装置; 来自照明装置的激发光的光谱（55）具有在预定的激发波长（λ1）处具有在激发（λ1）和吸收（λ2）波长之间的预定差异的波峰（55'），由此， 使得所述荧光团剂发出的光与来自照明装置的光容易。