公开(公告)号：US20240309195A1

公开(公告)日：2024-09-19

申请号：US18578419

申请日：2022-08-22

Applicant: EMD Millipore Corporation

Inventor： Kevin Wu ,  Kelly Wolfe ,  Anja Dedeo ,  Ryan Amara ,  Gerard Libby ,  Paul Sydlowski ,  Katelyn Kavanaugh ,  Trisha Bailey ,  Nathan T. Allen

IPC: C08L33/26 ,  C08J9/28 ,  G01N27/447

CPC classification number: C08L33/26 ,  C08J9/28 ,  G01N27/44747 ,  C08J2333/26

Abstract: An acrylamide gel formulation for use in electrophoresis, and a system and method for electrophoretic gel casting. In some embodiments, the system comprises a UV-Vis curing light source, and an acrylamide formulation that allows a resolving gel and a stacking gel to be photopolymerized in a single step. In some embodiments, the resolving solution formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethylbenzoyl-phosphinate (LAP) as a water-soluble photoinitiator, which allows the solution to be photopolymerized using UV radiation within a certain wavelength. In some embodiments, a dilution buffer formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethyl-benzoyl-phosphinate (LAP). The dilution buffer may be added to the resolving solution in a selected amount in order to modify the concentration of the acrylamide to a desired concentration.