公开(公告)号：US20240309195A1

公开(公告)日：2024-09-19

申请号：US18578419

申请日：2022-08-22

申请人： EMD Millipore Corporation

发明人： Kevin Wu ,  Kelly Wolfe ,  Anja Dedeo ,  Ryan Amara ,  Gerard Libby ,  Paul Sydlowski ,  Katelyn Kavanaugh ,  Trisha Bailey ,  Nathan T. Allen

IPC分类号： C08L33/26 ,  C08J9/28 ,  G01N27/447

CPC分类号： C08L33/26 ,  C08J9/28 ,  G01N27/44747 ,  C08J2333/26

摘要： An acrylamide gel formulation for use in electrophoresis, and a system and method for electrophoretic gel casting. In some embodiments, the system comprises a UV-Vis curing light source, and an acrylamide formulation that allows a resolving gel and a stacking gel to be photopolymerized in a single step. In some embodiments, the resolving solution formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethylbenzoyl-phosphinate (LAP) as a water-soluble photoinitiator, which allows the solution to be photopolymerized using UV radiation within a certain wavelength. In some embodiments, a dilution buffer formulation comprises acrylamide and bis-acrylamide, Bis-Tris buffering agent, and lithium phenyl-2, 4, 6-trimethyl-benzoyl-phosphinate (LAP). The dilution buffer may be added to the resolving solution in a selected amount in order to modify the concentration of the acrylamide to a desired concentration.

公开(公告)号：US20240192166A1

公开(公告)日：2024-06-13

申请号：US18533928

申请日：2023-12-08

申请人： EMD Millipore Corporation

发明人： Kurt E. Greenizen ,  Ryan Amara ,  Christopher A. Scott ,  Paul Sydlowski ,  Michael Miller ,  Kelly Wolfe

IPC分类号： G01N27/447

CPC分类号： G01N27/44743

摘要： A removal tool for sample well combs that is configured to aid in the extraction of a sample well comb from an electrophoresis gel. The removal tool may be either removably coupled to the sample well comb or may be permanently affixed to the sample well comb in order to provide a surface/region that is more easily graspable by a user to aid in the extraction of the sample well comb. The removal tool may include one or more engagement members that may be at least partially disposed within an opening of the sample well comb to couple the removal tool to the sample well comb. The removal tool may also include a mechanism that is configured to impart extraction forces to a surface in proximity to the sample well comb to further aid in the extraction of the sample well comb.