公开(公告)号：US11001866B2

公开(公告)日：2021-05-11

申请号：US16620529

申请日：2017-10-25

Applicant: JIANGSU AOSAIKANG PHARMACEUTICAL CO., LTD. ,  EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Huilei Yu ,  Qian Zhao ,  Yan Zhang ,  Jiang Pan ,  Jianhe Xu

IPC: C12P17/16 ,  C12R1/01

Abstract: A strain of Burkholderia is Burkholderia glathei ECU0712, with an accession number of CGMCC NO. 14464. With the strain or its extract as the biocatalyst, thioether is catalyzed to be oxidized asymmetrically to chiral sulfoxide, with significant advantages that the obtained product has a high optical purity, and benefits of a simple reaction system, short preparation time of the catalyst and a high yield of the product.

公开(公告)号：US10787651B2

公开(公告)日：2020-09-29

申请号：US16620525

申请日：2018-04-13

Applicant: JIANGSU AOSAIKANG PHARMACEUTICAL CO., LTD. ,  EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Huilei Yu ,  Yan Zhang ,  Jianhe Xu ,  Qian Zhao ,  Jiang Pan ,  Feng Liu ,  Guoqiang Lin

IPC: C12N9/02 ,  C12P17/02 ,  C12P11/00 ,  C12P17/10 ,  C12P17/16 ,  A61K38/00

Abstract: A Bradyrhizobium monooxygenase, a gene for encoding the monooxygenase, a recombinant expression vector comprising the gene and a recombinant transformant, a method of preparing the monooxygenase by the recombinant expression transformant, and a method of preparing an optically pure chiral sulfoxide by the monooxygenase, in particular to a method of preparing prazole drugs by means of catalyzing the asymmetric oxidation of thioether, a prazole precursor. As compared with other methods of preparing an optically pure sulfoxide, the product produced by the monooxygenase of the present invention as a catalyst has high optical purity, avoids the generation of the byproduct sulfone, and has advantages of mild reaction conditions, simple and convenient operations, easy amplification, etc.

公开(公告)号：US11306259B2

公开(公告)日：2022-04-19

申请号：US16665233

申请日：2019-10-28

Applicant: East China University of Science and Technology

Inventor： Zhishan Bai ,  Yan Zhang ,  Xiaoyong Yang ,  Huiqing Luo

IPC: C10G29/28 ,  B01D3/06 ,  C07C2/62 ,  C07C7/00 ,  C10G57/00

Abstract: The present disclosure relates to a method and an apparatus for self-heat-extracting flash evaporation of a sulfuric acid alkylation reaction product. There is provided a method for self-heat-extracting flash evaporation of a sulfuric acid alkylation reaction product. One step is to coalesce and vaporize a preliminarily distributed sulfuric acid alkylation reaction product to cause preliminary vaporization of a hydrocarbon therein, thereby taking heat away and preliminarily separating the hydrocarbon from sulfuric acid. Another step is to subject the preliminarily separated alkylation reaction to reinforced separation, where the hydrocarbon is further vaporized to take heat away and further separate the hydrocarbon from the sulfuric acid. There is also provided an apparatus for self-heat-extracting flash evaporation of a sulfuric acid alkylation reaction product.

公开(公告)号：US11345900B2

公开(公告)日：2022-05-31

申请号：US17294424

申请日：2019-11-26

Applicant: JIANGSU AOSAIKANG PHARMACEUTICAL CO., LTD. ,  EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Yan Zhang ,  Huilei Yu ,  Shimiao Ren ,  Peng Zhao ,  Jianhe Xu ,  Yinqi Wu ,  Qian Zhao

IPC: C12N9/02 ,  C12P17/14 ,  C12P17/16

Abstract: A monooxygenase having an amino acid sequence obtained by mutation of the amino acid sequence shown in SEQ ID NO:2 is disclosed. The use of the monooxygenase of the present invention in production of chiral sulfoxide-based drugs has advantages including mild reaction conditions, environmental friendliness, high yield, high optical purity of products, less peroxide products, and the like, and therefore the monooxygenase in the present invention has a good industrial application prospect in the production of proton pump inhibitors for the treatment of gastric ulcers.

公开(公告)号：US20200165530A1

公开(公告)日：2020-05-28

申请号：US16665233

申请日：2019-10-28

Applicant: East China University of Science and Technology

Inventor： Zhishan Bai ,  Yan Zhang ,  Xiaoyong Yang ,  Huiqing Luo

IPC: C10G29/28 ,  B01D3/06

Abstract: The present disclosure relates to a method and an apparatus for self-heat-extracting flash evaporation of a sulfuric acid alkylation reaction product. There is provided a method for self-heat-extracting flash evaporation of a sulfuric acid alkylation reaction product. One step is to coalesce and vaporize a preliminarily distributed sulfuric acid alkylation reaction product to cause preliminary vaporization of a hydrocarbon therein, thereby taking heat away and preliminarily separating the hydrocarbon from sulfuric acid. Another step is to subject the preliminarily separated alkylation reaction to reinforced separation, where the hydrocarbon is further vaporized to take heat away and further separate the hydrocarbon from the sulfuric acid. There is also provided an apparatus for self-heat-extracting flash evaporation of a sulfuric acid alkylation reaction product.