公开(公告)号：US5587287A

公开(公告)日：1996-12-24

申请号：US224218

申请日：1994-04-07

申请人： Edward R. Scalice ,  David J. Sharkey ,  Kenneth G. Christy, Jr. ,  Theodore W. Esders ,  John L. Daiss

发明人： Edward R. Scalice ,  David J. Sharkey ,  Kenneth G. Christy, Jr. ,  Theodore W. Esders ,  John L. Daiss

IPC分类号： C12N9/98 ,  C07K16/40 ,  C12N9/12 ,  C12N15/09 ,  C12P21/08 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/53 ,  C12P19/34 ,  C12Q1/70

CPC分类号： C12Q1/6804 ,  C07K16/40 ,  C12N9/1252 ,  C12Q1/6848

摘要： Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.

摘要翻译： 可以使用对热稳定性DNA聚合酶特异性的抗体来减少或消除聚合酶链反应方法中非特异性产物的形成。 这些抗体和其他温度敏感的抑制剂在通常低于约85℃的一定温度T1下有效抑制DNA聚合酶酶活性。抑制剂在通常高于约40℃的温度T2下是不可逆的灭活的.T2也更大 比T1。 这样的抑制剂可以在适合于PCR的诊断测试试剂盒中单独提供或与DNA聚合酶混合提供。

公开(公告)号：US5338671A

公开(公告)日：1994-08-16

申请号：US958144

申请日：1992-10-07

申请人： Edward R. Scalice ,  David J. Sharkey ,  Kenneth G. Christy, Jr. ,  Theodore W. Esders ,  John L. Daiss

发明人： Edward R. Scalice ,  David J. Sharkey ,  Kenneth G. Christy, Jr. ,  Theodore W. Esders ,  John L. Daiss

IPC分类号： C12N9/98 ,  C07K16/40 ,  C12N9/12 ,  C12N15/09 ,  C12P21/08 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/53 ,  C12P19/34 ,  C07K15/28

CPC分类号： C12Q1/6804 ,  C07K16/40 ,  C12N9/1252 ,  C12Q1/6848

摘要： Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.

摘要翻译： 可以使用对热稳定性DNA聚合酶特异性的抗体来减少或消除聚合酶链反应方法中非特异性产物的形成。 这些抗体和其他温度敏感的抑制剂在通常低于约85℃的一定温度T1下有效抑制DNA聚合酶酶活性。抑制剂在通常高于约40℃的温度T2下是不可逆的灭活的.T2也更大 比T1。 这样的抑制剂可以在适合于PCR的诊断测试试剂盒中单独提供或与DNA聚合酶混合提供。

公开(公告)号：US5149416A

公开(公告)日：1992-09-22

申请号：US812885

申请日：1991-12-20

申请人： Hans W. Osterhoudt ,  Ignazio S. Ponticello ,  Kenneth G. Christy, Jr. ,  David B. LaTart

发明人： Hans W. Osterhoudt ,  Ignazio S. Ponticello ,  Kenneth G. Christy, Jr. ,  David B. LaTart

IPC分类号： C08F220/54 ,  G01N27/447

CPC分类号： G01N27/44747 ,  C08F220/54

摘要： Preformed, water-soluble, acrylamide based copolymers comprising a minor proportion of a comonomer, which comonomer contains a site for a crosslinking reaction with a selected crosslinking agent by a reaction that does not involve a free-radical vinyl addition mechanism, which copolymers have M.sub.w and M.sub.n values within selected ranges, can be conveniently and safely used to prepare electrophoresis gel media in situ.

摘要翻译： 包含少量共聚单体的预成型的水溶性丙烯酰胺基共聚物，该共聚单体含有通过不涉及自由基乙烯基加成机理的反应与所选择的交联剂进行交联反应的位点，该共聚物具有＆ 选择范围内的Mw和＆amp; Upbar＆Mn值可以方便，安全地用于原位制备电泳凝胶介质。

公开(公告)号：US5066376A

公开(公告)日：1991-11-19

申请号：US430998

申请日：1989-11-02

申请人： Hans W. Osterhoudt ,  Ignazio S. Ponticello ,  Kenneth G. Christy, Jr. ,  Wayne A. Bowman ,  Jon N. Eikenberry

发明人： Hans W. Osterhoudt ,  Ignazio S. Ponticello ,  Kenneth G. Christy, Jr. ,  Wayne A. Bowman ,  Jon N. Eikenberry

IPC分类号： G01N27/447

CPC分类号： G01N27/44747

摘要： A polyacrylamide type gel medium for electrophoresis is conveniently and safely prepared prior to use by crosslinking a water-soluble copolymer of an acrylamide monomer and at least one comonomer copolymerizable therewith, which comonomer contains an active methylene group that will enter into a crosslinking reaction that does not involve a free radical vinyl addition mechanism.

摘要翻译： 用于电泳的聚丙烯酰胺型凝胶介质在使用前通过交联丙烯酰胺单体和至少一种可与其共聚的共聚单体的水溶性共聚物交联而方便和安全地制备，该共聚单体含有活性亚甲基，其将进入交联反应 不涉及自由基乙烯基添加机理。

公开(公告)号：US4948480A

公开(公告)日：1990-08-14

申请号：US188821

申请日：1988-05-02

申请人： Kenneth G. Christy, Jr. ,  Hans W. Osterhoudt ,  Ignazio S. Ponticello

发明人： Kenneth G. Christy, Jr. ,  Hans W. Osterhoudt ,  Ignazio S. Ponticello

IPC分类号： C07K1/26 ,  C08F20/52 ,  C08F220/56 ,  C08L33/24 ,  C08L33/26 ,  C25D13/04 ,  G01N27/447

CPC分类号： G01N27/44747

摘要： A polyacrylamide type gel medium for electrophoresis is conveniently and safely prepared prior to use from a kit which comprises (1) a water-soluble copolymer of an acrylamide monomer and at least one comonomer copolymerizable therewith, which comonomer contains functional groups that permit crosslinking of said copolymer by a covalent but non-free radical initiated mechanism; and (2) a selected crosslinking agent in a package that separates it from the copolymer. Optionally, additional materials that may be usefully incorporated in the gel may be included in the kit, for example, a selected electrophoresis buffer and a complexing agent, such as SDS. The kit is used to prepare the gel by forming a solution of the copolymer in deionized water at the desired concentration of copolymer, adding a sufficient amount of any optional ingredients to provide the desired concentrations thereof, adding such concentration of the crosslinking agent as will provide a gelation time of between about 5 minutes and about 15 hours, and pouring the so prepared reaction mixture into a preselected mold or casting device or onto a support so as to form the resulting gel into the desired shape and size.