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1.发明授权Thermostable polymerase specific antibody-containing DNA amplification composition and kit 失效耐热聚合酶特异性抗体的DNA扩增组合物和试剂盒
公开(公告)号：US5587287A
公开(公告)日：1996-12-24
申请号：US224218
申请日：1994-04-07
申请人： Edward R. Scalice , David J. Sharkey , Kenneth G. Christy, Jr. , Theodore W. Esders , John L. Daiss
发明人： Edward R. Scalice , David J. Sharkey , Kenneth G. Christy, Jr. , Theodore W. Esders , John L. Daiss
IPC分类号： C12N9/98 , C07K16/40 , C12N9/12 , C12N15/09 , C12P21/08 , C12Q1/68 , C12R1/91 , G01N33/53 , C12P19/34 , C12Q1/70
CPC分类号： C12Q1/6804 , C07K16/40 , C12N9/1252 , C12Q1/6848
摘要： Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.
摘要翻译： 可以使用对热稳定性DNA聚合酶特异性的抗体来减少或消除聚合酶链反应方法中非特异性产物的形成。 这些抗体和其他温度敏感的抑制剂在通常低于约85℃的一定温度T1下有效抑制DNA聚合酶酶活性。抑制剂在通常高于约40℃的温度T2下是不可逆的灭活的.T2也更大 比T1。 这样的抑制剂可以在适合于PCR的诊断测试试剂盒中单独提供或与DNA聚合酶混合提供。
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2.发明授权DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody 失效用热稳定DNA聚合酶和聚合酶抑制抗体进行DNA扩增
公开(公告)号：US5338671A
公开(公告)日：1994-08-16
申请号：US958144
申请日：1992-10-07
申请人： Edward R. Scalice , David J. Sharkey , Kenneth G. Christy, Jr. , Theodore W. Esders , John L. Daiss
发明人： Edward R. Scalice , David J. Sharkey , Kenneth G. Christy, Jr. , Theodore W. Esders , John L. Daiss
IPC分类号： C12N9/98 , C07K16/40 , C12N9/12 , C12N15/09 , C12P21/08 , C12Q1/68 , C12R1/91 , G01N33/53 , C12P19/34 , C07K15/28
CPC分类号： C12Q1/6804 , C07K16/40 , C12N9/1252 , C12Q1/6848
摘要： Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.
摘要翻译： 可以使用对热稳定性DNA聚合酶特异性的抗体来减少或消除聚合酶链反应方法中非特异性产物的形成。 这些抗体和其他温度敏感的抑制剂在通常低于约85℃的一定温度T1下有效抑制DNA聚合酶酶活性。抑制剂在通常高于约40℃的温度T2下是不可逆的灭活的.T2也更大 比T1。 这样的抑制剂可以在适合于PCR的诊断测试试剂盒中单独提供或与DNA聚合酶混合提供。
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公开(公告)号：US06838250B2
公开(公告)日：2005-01-04
申请号：US09821227
申请日：2001-03-29
申请人： Edward R. Scalice , John L. Daiss
发明人： Edward R. Scalice , John L. Daiss
IPC分类号： G01N33/53 , C07K16/18 , C07K16/42 , C12N5/10 , C12N5/20 , C12P21/08 , G01N33/543 , G01N33/577 , G01N33/68
CPC分类号： G01N33/686 , C07K16/18 , C07K16/4241 , G01N33/68 , G01N2333/4737 , Y10S435/965 , Y10S435/975
摘要： The present invention relates to new CRP immunoassay compositions. The compositions include a low affinity anti-human CRP monoclonal antibody, and an antiidiotypic antibody raised against it. The invention further provides a method for obtaining antiidiotypic monoclonal antibody populations directed to an antibody that is specific for a high concentration, high molecular weight target antigen.
摘要翻译： 本发明涉及新的CRP免疫测定组合物。 组合物包括低亲和力的抗人CRP单克隆抗体和针对其产生的抗独特型抗体。 本发明还提供了获得针对高浓度，高分子量靶抗原特异性抗体的抗独特型单克隆抗体群体的方法。
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4.发明授权Specific binding assay using enzyme inhibitor and anti-inhibitor antibodies 失效使用酶抑制剂和抗抑制剂抗体的特异性结合测定
公开(公告)号：US5916757A
公开(公告)日：1999-06-29
申请号：US683247
申请日：1996-07-17
CPC分类号： G01N33/581
摘要： Specific binding ligands can be detected with an assay which utilizes an immobilized receptor for the ligand, an immobilized reporter enzyme, an inhibitor antibody and a a water-soluble conjugate of the ligand and an anti-inhibitor antibody. Both antibodies are specific for the reporter enzyme, but the antibodies affect enzymatic activity differently. The inhibitor antibody effectively shuts down the activity of the reporter enzyme when it is complexed thereto. The anti-inhibitor antibody binds to the reporter enzyme, does not affect the enzymatic activity, but prevents the binding of the inhibitor enzyme. This assay provides a direct correlation of the generated signal to the target specific binding ligand.
摘要翻译： 可以用利用配体的固定化受体，固定的报道酶，抑制剂抗体和配体的水溶性缀合物和抗抑制剂抗体的测定法来检测特异性结合配体。 两种抗体对于报道酶是特异的，但抗体对不同的酶活性影响。 当抑制剂抗体与其复合时，抑制剂抗体有效地抑制了报道酶的活性。 抗抑制剂抗体与报道酶结合，不影响酶活性，但不会阻止抑制酶的结合。 该测定提供所产生的信号与靶特异性结合配体的直接相关性。
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公开(公告)号：US09737601B2
公开(公告)日：2017-08-22
申请号：US13696019
申请日：2011-05-03
CPC分类号： A61K39/40 , A61F2/28 , A61F2/30 , A61K45/06 , A61K2039/505 , C07K16/1271 , C07K2317/24 , C07K2317/56 , C07K2317/76
摘要： The present invention is directed to a monoclonal antibody that binds specifically to a Staphylococcus aureus glucosaminidase and inhibits in vivo growth of S. aureus. Also disclosed are monoclonal antibody binding portions, recombinant or hybridoma cell lines, pharmaceutical compositions containing the monoclonal antibody or binding portions thereof, and methods of treating 5*. aureus infection and osteomyelitis, and methods for introducing an orthopedic implant into a patient using the monoclonal antibody, binding portion, or pharmaceutical composition of the present invention.
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公开(公告)号：US5191068A
公开(公告)日：1993-03-02
申请号：US571619
申请日：1990-09-04
IPC分类号： G01N33/531 , C07K1/16 , C07K1/22 , C07K14/705 , C07K16/00 , G01N33/538 , G01N33/543 , G01N33/569 , G01N33/577
CPC分类号： G01N33/538 , G01N33/56972
摘要： A method of removing an antigenic substance from a fluid comprises(1) forming a ternary complex by the interaction of(a) the antigenic substance,(b) a first antibody which contains a kappa chain and which binds to the antigenic substance, and(c) a second antibody which binds to the kappa chain of the first antibody, said second antibody being immobilized on a solid phase carrier, and(2) separating the fluid from the solid phase carrier.
摘要翻译： PCT No.PCT / GB90 / 00012 Sec。 371 1990年9月4日第 102（e）1990年9月4日PCT PCT 1990年1月4日PCT公布。 出版物WO90 / 07715 日本1990年7月12日。从流体中除去抗原物质的方法包括（1）通过（a）抗原物质，（b）含有κ链的第一抗体与其结合的形式形成三元复合物 和（c）结合第一抗体的κ链的第二抗体，所述第二抗体固定在固相载体上，和（2）从固相载体分离流体。
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公开(公告)号：US4950592A
公开(公告)日：1990-08-21
申请号：US048847
申请日：1987-05-12
申请人： John L. Daiss
发明人： John L. Daiss
IPC分类号： C07K14/195 , C07K14/005 , C07K16/00 , C07K16/40 , C12N5/10 , C12N15/02 , C12P21/00 , C12P21/08 , C12R1/91 , G01N33/573 , G01N33/577
CPC分类号： C07K16/40 , Y10S530/861
摘要： A blend of monoclonal antibodies is disclosed. The blend inhibits the activity of creatine kinase-MM at least 99.5 percent. The blend is useful in determining the level of creatine kinase-MB activity in biological fluids.
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8.发明申请公开(公告)号：US20130110249A1
公开(公告)日：2013-05-02
申请号：US13696019
申请日：2011-05-03
CPC分类号： A61K39/40 , A61F2/28 , A61F2/30 , A61K45/06 , A61K2039/505 , C07K16/1271 , C07K2317/24 , C07K2317/56 , C07K2317/76
摘要： The present invention is directed to a monoclonal antibody that binds specifically to a Staphylococcus aureus glucosaminidase and inhibits in vivo growth of S. aureus. Also disclosed are monoclonal antibody binding portions, recombinant or hybridoma cell lines, pharmaceutical compositions containing the monoclonal antibody or binding portions thereof, and methods of treating 5*. aureus infection and osteomyelitis, and methods for introducing an orthopedic implant into a patient using the monoclonal antibody, binding portion, or pharmaceutical composition of the present invention.
摘要翻译： 本发明涉及特异性结合金黄色葡萄球菌氨基葡糖苷酶并抑制金黄色葡萄球菌体内生长的单克隆抗体。 还公开了单克隆抗体结合部分，重组或杂交瘤细胞系，含有单克隆抗体的药物组合物或其结合部分，以及治疗5 *的方法。 金黄色葡萄球菌感染和骨髓炎，以及使用本发明的单克隆抗体，结合部分或药物组合物将矫形植入物引入患者的方法。
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9.发明授权
公开(公告)号：US5565326A
公开(公告)日：1996-10-15
申请号：US250883
申请日：1994-05-31
IPC分类号： G01N33/531 , C12Q1/00 , C12Q1/28 , G01N33/543 , G01N33/58 , G01N33/53
CPC分类号： G01N33/54306 , G01N33/581 , Y10S435/962 , Y10S435/969 , Y10S435/97 , Y10S435/975 , Y10S436/816 , Y10S436/822
摘要： Specific binding ligands can be detected with an assay which utilizes an immobilized receptor for the ligand, a reporter enzyme, an inhibitor antibody and an anti-inhibitor antibody. Both antibodies are specific for the reporter enzyme. The inhibitor antibody effectively shuts down the activity of the reporter enzyme when it is complexed thereto. The anti-inhibitor antibody binds to the reporter enzyme, does not affect the enzymatic activity, but prevents the binding of the inhibitor enzyme. This assay provides a direct correlation of the target specific binding ligand to the signal generated without the use of separation or wash steps.
摘要翻译： 可以通过使用固定化受体作为配体，报道酶，抑制剂抗体和抗抑制剂抗体的测定来检测特异性结合配体。 两种抗体对报道酶都是特异性的。 当抑制剂抗体与其复合时，抑制剂抗体有效地抑制了报道酶的活性。 抗抑制剂抗体与报道酶结合，不影响酶活性，但不会阻止抑制酶的结合。 该测定提供目标特异性结合配体与不使用分离或洗涤步骤产生的信号的直接相关性。
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公开(公告)号：US4933291A
公开(公告)日：1990-06-12
申请号：US95693
申请日：1987-09-14
申请人： John L. Daiss , Leonard J. Seaberg , Alan J. Lowne
发明人： John L. Daiss , Leonard J. Seaberg , Alan J. Lowne
CPC分类号： B01L3/0275 , G01N2035/00495 , Y10T436/111666 , Y10T436/25375
摘要： There are described a novel removable pipette tip and pipette therefor, which provide separation of substances of a liquid within the tip by centrifuging the tip. The tip comprises a liquid-confining cavity disposed about an axis of symmetry, a dispensing aperture, and separating structure spaced along the axis away from the dispensing aperture, for separating one of the liquid substances when the tip is spun about such axis. The pipette is rotatable and either the pipette or the analyzer includes a driver for spinning the pipette tip at high speed.
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