公开(公告)号：US5587287A

公开(公告)日：1996-12-24

申请号：US224218

申请日：1994-04-07

申请人： Edward R. Scalice ,  David J. Sharkey ,  Kenneth G. Christy, Jr. ,  Theodore W. Esders ,  John L. Daiss

发明人： Edward R. Scalice ,  David J. Sharkey ,  Kenneth G. Christy, Jr. ,  Theodore W. Esders ,  John L. Daiss

IPC分类号： C12N9/98 ,  C07K16/40 ,  C12N9/12 ,  C12N15/09 ,  C12P21/08 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/53 ,  C12P19/34 ,  C12Q1/70

CPC分类号： C12Q1/6804 ,  C07K16/40 ,  C12N9/1252 ,  C12Q1/6848

摘要： Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.

摘要翻译： 可以使用对热稳定性DNA聚合酶特异性的抗体来减少或消除聚合酶链反应方法中非特异性产物的形成。 这些抗体和其他温度敏感的抑制剂在通常低于约85℃的一定温度T1下有效抑制DNA聚合酶酶活性。抑制剂在通常高于约40℃的温度T2下是不可逆的灭活的.T2也更大 比T1。 这样的抑制剂可以在适合于PCR的诊断测试试剂盒中单独提供或与DNA聚合酶混合提供。

公开(公告)号：US5338671A

公开(公告)日：1994-08-16

申请号：US958144

申请日：1992-10-07

申请人： Edward R. Scalice ,  David J. Sharkey ,  Kenneth G. Christy, Jr. ,  Theodore W. Esders ,  John L. Daiss

发明人： Edward R. Scalice ,  David J. Sharkey ,  Kenneth G. Christy, Jr. ,  Theodore W. Esders ,  John L. Daiss

IPC分类号： C12N9/98 ,  C07K16/40 ,  C12N9/12 ,  C12N15/09 ,  C12P21/08 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/53 ,  C12P19/34 ,  C07K15/28

CPC分类号： C12Q1/6804 ,  C07K16/40 ,  C12N9/1252 ,  C12Q1/6848

摘要： Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T.sub.1 which is generally below about 85.degree. C. The inhibitors are irreversibly inactivated at temperature T.sub.2 which is generally above about 40.degree. C. T.sub.2 is also greater than T.sub.1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.

摘要翻译： 可以使用对热稳定性DNA聚合酶特异性的抗体来减少或消除聚合酶链反应方法中非特异性产物的形成。 这些抗体和其他温度敏感的抑制剂在通常低于约85℃的一定温度T1下有效抑制DNA聚合酶酶活性。抑制剂在通常高于约40℃的温度T2下是不可逆的灭活的.T2也更大 比T1。 这样的抑制剂可以在适合于PCR的诊断测试试剂盒中单独提供或与DNA聚合酶混合提供。

公开(公告)号：US5622822A

公开(公告)日：1997-04-22

申请号：US305769

申请日：1994-09-13

申请人： Tobias E. Ekeze ,  John W. Backus ,  David J. Sharkey ,  Richard C. Sutton ,  JoAnne H. Kerschner

发明人： Tobias E. Ekeze ,  John W. Backus ,  David J. Sharkey ,  Richard C. Sutton ,  JoAnne H. Kerschner

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34 ,  C12Q1/70

CPC分类号： C12N15/1003 ,  C12Q1/6806

摘要： Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with polyethyleneimine to form a precipitate with the nucleic acids. The nucleic acids are then released from the precipitate by contact with a strong base, and the released nucleic acids are kept in solution with an anionic phosphate ester surfactant. This method for preparing specimen samples is simple and quite rapid.

摘要翻译： 通过使裂解物与聚乙烯亚胺接触以与核酸形成沉淀，可将核酸用于扩增或裂解后的其它处理。 然后通过与强碱接触将核酸从沉淀物中释放，并将释放的核酸与阴离子磷酸酯表面活性剂一起保持在溶液中。 这种制备样品样品的方法简单而且相当迅速。