公开(公告)号：US11781159B2

公开(公告)日：2023-10-10

申请号：US17068147

申请日：2020-10-12

Applicant: ExxonMobil Technology and Engineering Company

Inventor： Xiaozhou Zhang ,  Zarath M Summers ,  Partha Nandi ,  Mark P Hagemeister ,  Jihad M Dakka ,  Mohor Chatterjee ,  Vera Grankina

IPC: C12P7/42 ,  C07C51/377 ,  C12P19/02 ,  C12P19/14 ,  C13K1/00 ,  C12N1/16 ,  C07C51/367 ,  C07C59/01

CPC classification number: C12P7/42 ,  C07C51/377 ,  C12P19/02 ,  C12P19/14 ,  C13K1/00

Abstract: Hydroxycarboxylic acids may be biosynthesized from a carbonaceous feedstock and then isolated through forming and subsequently hydrolyzing an intermediate sophorolipid. After biosynthesizing a hydroxycarboxylic acid in a cell culture medium or otherwise providing a hydroxycarboxylic acid in a first aqueous medium, the hydroxycarboxylic acid and glucose may be converted into at least one sophorolipid by a suitable microorganism or an enzyme cocktail. The at least one sophorolipid may be then be separated from the cell culture medium or first aqueous medium and then hydrolyzed in a second aqueous medium to form the hydroxycarboxylic acid and glucose as free components separate from the cell culture medium or first aqueous medium. The hydroxycarboxylic acid is present as a phase separate from the second aqueous medium and the glucose remains in the second aqueous medium.