公开(公告)号：US11781159B2

公开(公告)日：2023-10-10

申请号：US17068147

申请日：2020-10-12

Applicant: ExxonMobil Technology and Engineering Company

Inventor： Xiaozhou Zhang ,  Zarath M Summers ,  Partha Nandi ,  Mark P Hagemeister ,  Jihad M Dakka ,  Mohor Chatterjee ,  Vera Grankina

IPC: C12P7/42 ,  C07C51/377 ,  C12P19/02 ,  C12P19/14 ,  C13K1/00 ,  C12N1/16 ,  C07C51/367 ,  C07C59/01

CPC classification number: C12P7/42 ,  C07C51/377 ,  C12P19/02 ,  C12P19/14 ,  C13K1/00

Abstract: Hydroxycarboxylic acids may be biosynthesized from a carbonaceous feedstock and then isolated through forming and subsequently hydrolyzing an intermediate sophorolipid. After biosynthesizing a hydroxycarboxylic acid in a cell culture medium or otherwise providing a hydroxycarboxylic acid in a first aqueous medium, the hydroxycarboxylic acid and glucose may be converted into at least one sophorolipid by a suitable microorganism or an enzyme cocktail. The at least one sophorolipid may be then be separated from the cell culture medium or first aqueous medium and then hydrolyzed in a second aqueous medium to form the hydroxycarboxylic acid and glucose as free components separate from the cell culture medium or first aqueous medium. The hydroxycarboxylic acid is present as a phase separate from the second aqueous medium and the glucose remains in the second aqueous medium.

公开(公告)号：US20230159978A1

公开(公告)日：2023-05-25

申请号：US17930462

申请日：2022-09-08

Applicant: ExxonMobil Technology and Engineering Company

Inventor： Xiaozhou Zhang ,  Mohor Chatterjee

IPC: C12Q1/28 ,  C12P7/04 ,  C12P7/24 ,  C12P3/00 ,  C12N9/04 ,  C12N9/88

CPC classification number: C12Q1/28 ,  C12P7/04 ,  C12P7/24 ,  C12P3/00 ,  C12N9/0006 ,  C12Y101/0302 ,  C12N9/88 ,  C12Y402/01053

Abstract: A variety of methods and systems for screening biocatalysts are disclosed, including, in one embodiment, a screening method for identifying engineered biocatalysts, including reacting an olefin with water in the presence of an engineered biocatalyst to produce at least a fatty alcohol having from 4 carbons to 24 carbons; reacting at least a portion of the fatty alcohol with oxygen in the present of a fatty alcohol oxidase to produce a fatty aldehyde and hydrogen peroxide, the fatty aldehyde having from 4 carbons to 24 carbons; and measuring activity of the engineered biocatalyst.