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公开(公告)号：US20240191265A1
公开(公告)日：2024-06-13
申请号：US18509425
申请日：2023-11-15
Inventor： Xiaozhou Zhang , Glen E. Alliger , Yuchen Liu
IPC: C12P7/62
CPC classification number: C12P7/62
Abstract: Methods of β-lactone production, including the integrated bioconversion of β-lactone production and methods related thereto. Methods include reacting a feedstock and a recombinant microorganism harboring one or more genes encoding enzymes involved in the metabolic pathway of a 3-hydroxycarboxylic acid, thereby producing a 3-hydroxycarboxylic acid having C1-C10 alkyl groups. Thereafter, reacting the 3-hydroxycarboxilic acid with a bioengineered natural β-lactone synthetase, the bioengineered β-lactone synthetase engineered to comprise a substrate binding pocket capable of accepting the 3-hydroxycarboxylic acid having C1-C10 alkyl groups, thereby producing a β-lactone having C1-C10 alkyl groups.
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公开(公告)号：US11781159B2
公开(公告)日：2023-10-10
申请号：US17068147
申请日：2020-10-12
Inventor： Xiaozhou Zhang , Zarath M Summers , Partha Nandi , Mark P Hagemeister , Jihad M Dakka , Mohor Chatterjee , Vera Grankina
IPC: C12P7/42 , C07C51/377 , C12P19/02 , C12P19/14 , C13K1/00 , C12N1/16 , C07C51/367 , C07C59/01
CPC classification number: C12P7/42 , C07C51/377 , C12P19/02 , C12P19/14 , C13K1/00
Abstract: Hydroxycarboxylic acids may be biosynthesized from a carbonaceous feedstock and then isolated through forming and subsequently hydrolyzing an intermediate sophorolipid. After biosynthesizing a hydroxycarboxylic acid in a cell culture medium or otherwise providing a hydroxycarboxylic acid in a first aqueous medium, the hydroxycarboxylic acid and glucose may be converted into at least one sophorolipid by a suitable microorganism or an enzyme cocktail. The at least one sophorolipid may be then be separated from the cell culture medium or first aqueous medium and then hydrolyzed in a second aqueous medium to form the hydroxycarboxylic acid and glucose as free components separate from the cell culture medium or first aqueous medium. The hydroxycarboxylic acid is present as a phase separate from the second aqueous medium and the glucose remains in the second aqueous medium.
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公开(公告)号：US20230159978A1
公开(公告)日：2023-05-25
申请号：US17930462
申请日：2022-09-08
Inventor： Xiaozhou Zhang , Mohor Chatterjee
CPC classification number: C12Q1/28 , C12P7/04 , C12P7/24 , C12P3/00 , C12N9/0006 , C12Y101/0302 , C12N9/88 , C12Y402/01053
Abstract: A variety of methods and systems for screening biocatalysts are disclosed, including, in one embodiment, a screening method for identifying engineered biocatalysts, including reacting an olefin with water in the presence of an engineered biocatalyst to produce at least a fatty alcohol having from 4 carbons to 24 carbons; reacting at least a portion of the fatty alcohol with oxygen in the present of a fatty alcohol oxidase to produce a fatty aldehyde and hydrogen peroxide, the fatty aldehyde having from 4 carbons to 24 carbons; and measuring activity of the engineered biocatalyst.
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公开(公告)号：US11590447B2
公开(公告)日：2023-02-28
申请号：US16660029
申请日：2019-10-22
Inventor： Mohsen S. Yeganeh , Pavel Kortunov , P. Scott Northrop , Xiaozhou Zhang , Giovanni Pilloni , Ning Ma , Robert J. Colby , Qiuzi Li , Zarath M. Summers , Matthew S. Ide
IPC: B01D53/14 , B01D53/22 , B01D67/00 , B01D69/14 , B01D53/18 , B01D53/26 , B01J20/08 , B01J20/10 , B01J20/22 , B01J20/26 , B01J20/28 , B01J21/08 , B01J31/00 , B01J31/26 , B01J35/06 , C02F1/26 , C02F3/34 , C02F101/34
Abstract: The present disclosure relates to a porous liquid or a porous liquid enzyme that includes a high surface area solid and a liquid film substantially covering the high surface area solid. The porous liquid or porous liquid enzyme may be contacted with a fluid that is immiscible with the liquid film such that a liquid-fluid interface is formed. The liquid film may facilitate mass transfer of a substance or substrate across the liquid-fluid interface. The present disclosure also provides methods of performing liquid-based extractions and enzymatic reactions utilizing the porous liquid or porous liquid enzyme of the present disclosure.
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