公开(公告)号：US20130171729A1

公开(公告)日：2013-07-04

申请号：US13776053

申请日：2013-02-25

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Slawomir Rubinsztajn ,  Prameela Susarla ,  Reginald Donovan Smith ,  Evelina Roxana Loghin

IPC: C12N5/00

CPC classification number: C12N5/0068 ,  C12M23/20 ,  C12M33/00 ,  C12N2533/32 ,  C12N2539/10

Abstract: Methods and kits of releasing cells are provided. The method comprises the steps of providing cultured cells on a cell culture support comprising a multi layer polyelectrolyte coating immobilized on a substrate, and releasing the cultured cells from the cell culture support by a releasing solution comprising DMSO. The kit comprises a cell culture support and a releasing solution. The releasing solution comprises DMSO.

Abstract translation: 提供了释放细胞的方法和试剂盒。 该方法包括以下步骤：在包含固定在底物上的多层聚电解质涂层的细胞培养载体上提供培养的细胞，以及通过包含DMSO的释放溶液从细胞培养支持物释放培养的细胞。 该试剂盒包括细胞培养支持物和释放溶液。 释放溶液包含DMSO。

公开(公告)号：US20160376550A1

公开(公告)日：2016-12-29

申请号：US15262644

申请日：2016-09-12

Applicant: General Electric Company

Inventor： Reginald Donovan Smith ,  Prameela Susarla ,  Slawomir Rubinsztajn ,  Brian David Polizzotti ,  Anton Beletskii

IPC: C12N5/00 ,  C12M1/26 ,  C08F8/12 ,  C12M1/00

CPC classification number: C12N5/0075 ,  C08F8/12 ,  C08F293/005 ,  C12M23/20 ,  C12M33/00 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2539/10

Abstract: A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.

Abstract translation: 一种细胞培养支持物，其包含底物和固定在底物上的双重刺激响应性嵌段共聚物，其中所述双重刺激响应性嵌段共聚物是热反应性和pH响应性的。 一种培养细胞的方法，所述细胞包含具有固定在底物上的双重刺激响应共聚物的细胞培养支持物，其中所述双重刺激响应共聚物是热反应性和pH响应性的; 并在细胞培养支持下培养细胞。 通过降低温度，细胞从细胞培养支持物释放。

公开(公告)号：US10131874B2

公开(公告)日：2018-11-20

申请号：US15262644

申请日：2016-09-12

Applicant: General Electric Company

Inventor： Reginald Donovan Smith ,  Prameela Susarla ,  Slawomir Rubinsztajn ,  Brian David Polizzotti ,  Anton Beletskii

IPC: C12N5/00 ,  C08F293/00 ,  C12M1/26 ,  C12M1/00 ,  C08F8/12

Abstract: A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.

公开(公告)号：US08993322B2

公开(公告)日：2015-03-31

申请号：US13776053

申请日：2013-02-25

Applicant: General Electric Company

Inventor： Slawomir Rubinsztajn ,  Prameela Susarla ,  Reginald Donovan Smith ,  Evelina Roxana Loghin

IPC: C12N5/077 ,  C12N5/00

CPC classification number: C12N5/0068 ,  C12M23/20 ,  C12M33/00 ,  C12N2533/32 ,  C12N2539/10

Abstract: Methods and kits of releasing cells are provided. The method comprises the steps of providing cultured cells on a cell culture support comprising a multi layer polyelectrolyte coating immobilized on a substrate, and releasing the cultured cells from the cell culture support by a releasing solution comprising DMSO. The kit comprises a cell culture support and a releasing solution. The releasing solution comprises DMSO.

Abstract translation: 提供了释放细胞的方法和试剂盒。 该方法包括以下步骤：在包含固定在底物上的多层聚电解质涂层的细胞培养载体上提供培养的细胞，以及通过包含DMSO的释放溶液从细胞培养支持物释放培养的细胞。 该试剂盒包括细胞培养支持物和释放溶液。 释放溶液包含DMSO。