公开(公告)号：US20190241857A1

公开(公告)日：2019-08-08

申请号：US16261249

申请日：2019-01-29

申请人： Chung Yuan Christian University

发明人： Tzong-Rong Ger ,  Tan-Yueh Chen ,  Chao-Ming Su ,  Yin-Chou Huang ,  Fan Dong ,  Yu-Chi Kuo

IPC分类号： C12M1/26 ,  C12M1/12 ,  C12M1/42 ,  C12N5/00 ,  C12M1/34

CPC分类号： C12M33/00 ,  C12M25/14 ,  C12M35/06 ,  C12M41/12 ,  C12N5/0068 ,  C12N2523/00 ,  C12N2527/00 ,  C12N2533/30 ,  C12N2535/10 ,  C12N2537/00

摘要： A device and method for cultivating cells are provided, wherein a cell cultivating layer is formed on a surface of a substrate, and a temperature-responsive layer having a plurality of temperature-responsive polymer with magnetic objects is formed on a surface of the cell cultivating layer. When a controlling characteristic exerted on the temperature-responsive layer is varied, a plurality of cells can be adhered on the cell cultivating layer or detached therefrom. When the device is utilized, a temperature control step is operated to control environmental temperature at a first temperature for inducing temperature-responsive polymers becoming hydrophobic whereby the plurality of cells are adhered on the cell cultivating layer. In addition, the alternating magnetic field is utilized to rise temperature of the temperature-responsive polymers so that the plurality of cells can be kept being adhered on the cell cultivating layer at a second temperature lower than the first temperature.

公开(公告)号：US20190241849A1

公开(公告)日：2019-08-08

申请号：US16329779

申请日：2017-08-30

申请人： University of Kansas

发明人： Adam Joseph Mellott

IPC分类号： C12M1/32 ,  C12N5/00 ,  C12M3/00 ,  C12M1/12

CPC分类号： C12M23/12 ,  C08B37/003 ,  C08B37/0072 ,  C08B37/0084 ,  C12M21/08 ,  C12M23/04 ,  C12N5/0062 ,  C12N5/0068 ,  C12N2513/00 ,  C12N2535/00

摘要： Expandable cell culture substrates are provided that allow continual expansion of adherent stem cells without the need to passage cells, thereby preserving health and stem cell characteristics. The expandable cell culture substrates can include various three-dimensional structures having various coupling mechanisms that allow the structures to be joined, including where the structures are configured as interlocking blocks made from a variety of materials. The three-dimensional structures can include various coupling points, such as protrusions and opposing indentions, to allow for interlocking of the structures in the x-, y-, and z-axial directions. As a cell culture reaches a particular density, additional units can be added to increase the surface area of the expandable cell culture substrate.

公开(公告)号：US20190218507A1

公开(公告)日：2019-07-18

申请号：US16250457

申请日：2019-01-17

申请人： THE SECANT GROUP, LLC

发明人： Peter D. GABRIELE ,  Jeremy J. HARRIS ,  Charles Brendan NICHOLSON ,  Steven LU ,  Jeffrey H. ROBERTSON ,  Gael PERON

IPC分类号： C12N5/00 ,  C12M1/00 ,  C12M1/12

CPC分类号： C12N5/0068 ,  C12M23/14 ,  C12M23/20 ,  C12M23/26 ,  C12M23/28 ,  C12M23/52 ,  C12M25/14 ,  C12M41/46 ,  C12N2533/30

摘要： A biocontainment vessel includes a vessel structure including a structural composition and an enhancement composition associated with the structural composition. The enhancement composition includes a co-polymer. The co-polymer is a poly(glycerol sebacate) or a poly(glycerol sebacate urethane). The enhancement composition may also include an augmentation agent associated with the co-polymer. The enhancement composition is located with respect to the structural composition such that the enhancement composition benefits biological cells contained in the biocontainment vessel. A composition includes a co-polymer and an augmentation agent contained by the co-polymer. A method of containing biological cells includes placing the biological cells in an augmented biocontainment vessel and storing them in the augmented biocontainment vessel under predetermined conditions. An augmented substrate includes a substrate and an enhancement composition coating a surface of the substrate.

公开(公告)号：US20190201586A1

公开(公告)日：2019-07-04

申请号：US16312062

申请日：2017-06-23

申请人： Washington State University

发明人： Weimin Li ,  Girdhari Rijal

IPC分类号： A61L27/56 ,  A61K38/18 ,  A61L27/12 ,  C12N5/00 ,  C12Q1/02 ,  C12N5/071 ,  C12N5/077

CPC分类号： A61L27/56 ,  A61K35/12 ,  A61K38/18 ,  A61L27/12 ,  A61L27/227 ,  A61L27/3633 ,  A61L27/3683 ,  A61L27/52 ,  A61L2430/40 ,  C12N5/0062 ,  C12N5/0068 ,  C12N5/0629 ,  C12N5/0656 ,  C12N2533/90 ,  C12Q1/025

摘要： Provided are porous, hydrogel, and multilayer tissue matrix scaffolds that are derived from native tissues. The scaffolds can be used for cell culture, preparing tumoroids for in vivo implant, and testing or screening the efficacies or toxicities of drugs toward cancers or other diseases.

公开(公告)号：US20180355310A1

公开(公告)日：2018-12-13

申请号：US15780236

申请日：2016-11-30

申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

发明人： Jackie Y. YING ,  Nandanan ERATHODIYIL ,  Karthikeyan NARAYANAN ,  Andrew C.A. WAN

IPC分类号： C12N5/00

CPC分类号： C12N5/0068 ,  B82Y5/00 ,  B82Y30/00 ,  C12N2533/30 ,  C12N2537/00 ,  C12N2539/00

摘要： A cell culture substrate comprising a substrate having a coating of a plurality of amine functionalized nanoparticles is disclosed. In one embodiment, the amine functionalized nanoparticle is a polymer of an acrylamide monomer, a cross-linker and an amine monomer. There is also provided a method of making the cell culture substrate either by drying the amine functionalized nanoparticles when spread onto the said substrate or by covalent linkages of the substrate with thiol terminated nanoparticles. In addition, there is provided a method of culturing stem cells on the cell culture substrate having a coating of a plurality of the amine functionalized nanoparticles thereon.

公开(公告)号：US20180355309A1

公开(公告)日：2018-12-13

申请号：US15776912

申请日：2016-12-12

申请人： ZEON CORPORATION

发明人： Kazuki KUSABIRAKI ,  Naoya ICHIMURA

IPC分类号： C12N5/00

CPC分类号： C12N5/0068 ,  C12N2509/00 ,  C12N2533/30

摘要： The invention is a method for culturing adherent cells, culturing the adherent cells in a liquid medium in an alicyclic structure-containing polymer culture vessel, releasing the cells from the culture vessel by liquid flow without adding a protease, and suspending the cultured cells in a liquid medium. The cells can be easily released by a small force such as pipetting because the cells adhere loosely to the alicyclic structure-containing polymer culture vessel.

公开(公告)号：US20180305661A1

公开(公告)日：2018-10-25

申请号：US16020286

申请日：2018-06-27

申请人： Indiana University Research and Technology Corporation

发明人： Mervin C. Yoder ,  David A. Ingram

IPC分类号： C12N5/00 ,  C12N5/0789 ,  C12N5/071 ,  G01N33/569 ,  G01N33/50 ,  A61K35/12

CPC分类号： C12N5/0068 ,  A61K2035/124 ,  C12N5/0647 ,  C12N5/0692 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/26 ,  C12N2502/28 ,  C12N2533/54 ,  C12N2533/90 ,  G01N33/5005 ,  G01N33/56966 ,  G01N2333/70589 ,  G01N2333/70596

摘要： A hierarchy of endothelial colony forming cells (EPCs) was identified from mammalian cord blood, umbilical vein and aorta. A newly isolated cell named high proliferative potential endothelial colony forming cell (HPP-ECFC) was isolated and characterized. Single cell assays were developed that test the proliferative and clonogenic potential of endothelial cells derived from cord blood, or from HUVECs and HAECs. EPCs were found to reside in vessel walls. Use of a feeder layer of cells derived from high proliferative potential-endothelial colony forming cells (HPP-ECPCS) from human umbilical cord blood, stimulates growth and survival of repopulating hematopoietic stem and progenitor cells. Stimulation of growth and survival was determined by increased numbers of progenitor cells in in vitro cultures and increased levels of human cell engraftment in the NOD/SCID immunodeficient mouse transplant system.

公开(公告)号：US20180258960A9

公开(公告)日：2018-09-13

申请号：US15547320

申请日：2016-02-03

申请人： THE UNIVERSITY OF BRITISH COLUMBIA

发明人： Aziz Ghahary ,  Ryan Hartwell

IPC分类号： F15B1/16 ,  F15B1/24 ,  E21B33/064

CPC分类号： F15B1/165 ,  A61L27/26 ,  A61L27/48 ,  A61L27/52 ,  A61L27/60 ,  A61L2300/64 ,  A61L2400/06 ,  C12N5/0068 ,  C12N2533/54 ,  C12N2533/70 ,  C12N2537/10 ,  E21B33/064 ,  F15B1/24 ,  F15B2201/205 ,  F15B2201/31 ,  F15B2201/3152 ,  F15B2201/32 ,  F15B2201/41 ,  C08L89/06 ,  C08L29/04

摘要： The present invention is a new and improved bladder for use with a piston accumulator apparatus, system and method of using same that provides a dispersion tube in the bladder to allow to be communicated under a vacuum from the bladder to the accumulator seawater chamber and back again.

公开(公告)号：US20180256647A1

公开(公告)日：2018-09-13

申请号：US15976569

申请日：2018-05-10

申请人： Wake Forest University Health Sciences

发明人： Khalil Bitar

IPC分类号： A61K35/34 ,  A61F2/08 ,  C12N5/077 ,  A61L27/20 ,  A61L27/38 ,  C12N5/00 ,  A61F2/04 ,  A61L27/50 ,  C12N5/0797

CPC分类号： A61K35/34 ,  A61F2/04 ,  A61F2/08 ,  A61F2002/045 ,  A61K35/30 ,  A61L27/20 ,  A61L27/225 ,  A61L27/227 ,  A61L27/24 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3873 ,  A61L27/3886 ,  A61L27/50 ,  A61L2430/30 ,  C12N5/0068 ,  C12N5/0623 ,  C12N5/0661 ,  C12N5/0697 ,  C12N2501/999 ,  C12N2502/081 ,  C12N2502/1347 ,  C12N2533/54 ,  C12N2533/72 ,  C08L5/08

摘要： Methods of generating an innervated muscle structures are disclosed as well as bioengineered structures for tissue repair or regeneration. The methods can include the steps of obtaining populations of smooth muscle cells and neuronal progenitor cells and then seeding the cells together onto a matrix material, followed by culturing the seeded cells to form an innervated smooth muscle cell construct of directionally oriented smooth muscle cells. In one embodiment, the neuronal progenitor cells can be seeded first as neurospheres in a biocompatiable solution, e.g., a collagen/laminin solution, and allowed to gel. Next, a second suspension of smooth muscle cells can be deposited as separate layer. Multiple layer structures of alternating muscle or neuron composition can also be formed in this manner. Differentiation of the neuronal progenitor cells can be induced by exposure to a differentiation medium, such as Neurobasal A medium and/or exposure to a differentiating agent, such as B-27 supplement. The innervated muscle structures can be disposed around a tubular scaffold, e.g., a chitosan-containing tube and further cultured to form tubular, bioengineered structures and two or more innervated muscle structures can be joined together to form an elongate composite structure.

公开(公告)号：US10058807B2

公开(公告)日：2018-08-28

申请号：US15432047

申请日：2017-02-14

申请人： DONALDSON COMPANY, INC.

发明人： Andrew J. Dallas ,  William Lefei Ding ,  Jon D. Joriman ,  Dustin Zastera ,  James R. Giertz ,  Veli E. Kalayci ,  Hoo Y. Chung

IPC分类号： B01D39/16 ,  B01D39/04 ,  B01D39/14 ,  C12M1/12 ,  C12N5/00

CPC分类号： B01D39/1623 ,  B01D39/04 ,  B01D39/14 ,  B01D2239/0407 ,  B01D2239/0442 ,  B01D2239/0631 ,  B01D2239/064 ,  B01D2239/065 ,  B01D2239/1266 ,  C12M25/14 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/78 ,  Y02E50/17 ,  Y10T428/249921 ,  Y10T428/29 ,  Y10T442/614

摘要： The assemblies of the invention can comprise a fine fiber layer having dispersed within the fine fiber layer an active particulate material. Fluid that flows through the assemblies of the invention can have any material dispersed or dissolved in the fluid react with, be absorbed by, or adsorbed onto, the active particulate within the nanofiber layer. The structures of the invention can act simply as reactive, absorptive, or adsorptive layers with no filtration properties, or the structures of the invention can be assembled into filters that can filter particulate from a mobile fluid while simultaneously reacting, absorbing, or adsorbing materials from the mobile fluid.