公开(公告)号：US08546125B2

公开(公告)日：2013-10-01

申请号：US13031350

申请日：2011-02-21

申请人： Guo-Qiang Chen ,  Zheng-Jun Li ,  Zhen-Yu Shi ,  Ying-Ying Guo ,  Jia Jian ,  Qiong Wu

发明人： Guo-Qiang Chen ,  Zheng-Jun Li ,  Zhen-Yu Shi ,  Ying-Ying Guo ,  Jia Jian ,  Qiong Wu

IPC分类号： C12N1/20 ,  C12P9/00 ,  C07H21/04

CPC分类号： C12N9/88 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/625 ,  C12Y101/01036 ,  C12Y101/01061 ,  C12Y102/01016 ,  C12Y203/01009 ,  C12Y203/01016 ,  C12Y401/01001

摘要： Known attempts using engineered bacteria to produce P(3HB-co-4HB) with carbon sources that are structurally unrelated to 4-hydroxybutyrate resulted in relatively low 4HB monomer content of 1.5 to 5 mol %. The current invention provides recombinant hosts for producing P(3HB-co-4HB) wherein the plasmid including succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene) further includes pyruvate decarboxylase promoter (Ppdc). It was found that the 4HB monomer content in P(3HB-co-4HB) is significantly enhanced to be over 20 mol %, in the range of 8.8 to 23 mol %.

摘要翻译： 使用工程细菌产生具有与4-羟基丁酸盐结构无关的碳源的P（3HB-co-4HB）的已知尝试导致相对较低的4HB单体含量为1.5至5mol％。 本发明提供了用于产生P（3HB-co-4HB）的重组宿主，其中包含琥珀酸半醛脱氢酶基因（sucD基因）和4-羟基丁酸脱氢酶基因（4hbD基因）的质粒还包括丙酮酸脱羧酶启动子（Ppdc）。 发现P（3HB-co-4HB）中的4HB单体含量显着提高到超过20mol％，在8.8至23mol％的范围内。