公开(公告)号：US20240360425A1

公开(公告)日：2024-10-31

申请号：US18290561

申请日：2022-05-13

Applicant: Cellibre, Inc.

Inventor： Spiros Kambourakis ,  Nicholas Donald Keul ,  Russell Scott Komor ,  Jun Urano ,  Nicky Christopher Caiazza

IPC: C12N9/10 ,  C12P7/26

CPC classification number: C12N9/1029 ,  C12P7/26 ,  C12Y203/01086

Abstract: Described herein are the discovery and/or optimization of enzymes involved in the biosynthesis of olivetolic acid, divarinic acid, and analogs thereof.

公开(公告)号：US12129506B2

公开(公告)日：2024-10-29

申请号：US17809750

申请日：2022-06-29

Applicant: CARGILL, INCORPORATED

Inventor： Michael D. Lynch ,  Michael Tai Man Louie ,  Shelley Copley ,  Eileen Colie Spindler ,  Brittany Robinson ,  Matthew Lipscomb ,  Tanya Lipscomb-Warnecke ,  Hans H. Liao ,  David Hogsett ,  Ron Evans

IPC: C12P7/6409 ,  C12N1/20 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88

CPC classification number: C12P7/6409 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12Y101/01035 ,  C12Y103/01093 ,  C12Y203/01194 ,  C12Y402/01059 ,  C12Y203/01075 ,  C12Y301/02

Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism.

公开(公告)号：US12123045B2

公开(公告)日：2024-10-22

申请号：US17661613

申请日：2022-05-02

Applicant: CARGILL, INCORPORATED

Inventor： Hans H. Liao ,  Catherine Bradshaw Poor ,  Travis Robert Wolter ,  Michael Tai Man Louie ,  Erin Kathleen Marasco ,  Ana Negrete-Raymond

IPC: C12P7/6436 ,  C12N1/20 ,  C12N9/10 ,  C12P7/6409

CPC classification number: C12P7/6436 ,  C12N1/20 ,  C12N9/1029 ,  C12P7/6409 ,  C12Y203/01199

Abstract: Genes encoding mutant 3-ketoacyl-CoA synthases are introduced into host cells. Certain of the mutants enhance the production of shorter-chain fatty acids and derivatives by the cell than do the wild-type (unmutated) enzymes. In other cases, the chain length is not significantly affected, but productivity is enhanced. In specific cases, both a shift toward lower chain length and higher productivity is seen. Cells producing the mutant 3-ketoacyl-CoA synthases are especially suitable for producing C6-C10 fatty acids and derivatives.

公开(公告)号：US20240344076A1

公开(公告)日：2024-10-17

申请号：US18632652

申请日：2024-04-11

Applicant: Washington State University

Inventor： Philip D. Bates ,  Sajina Bhandari ,  Prasad Parchuri ,  Abdul Azeez

IPC: C12N15/82 ,  C12N9/10 ,  C12N9/20 ,  C12P7/42

CPC classification number: C12N15/8247 ,  C12N9/1029 ,  C12N9/20 ,  C12P7/42 ,  C12Y203/0102 ,  C12Y301/01003

Abstract: Method and associated transgenic oil-producing plants, protists, bacteria, and fungi for increasing concentration of target fatty acids for use in food, biofuel, or industrial chemical applications. Modification of organisms to enhance lipase expression induces triacylglycerol (TAG) remodeling to change the composition of stored oils. Lipase converts endogenously synthesized TAG back to diacylglycerols, allowing resynthesis into TAG with different fatty acid compositions using fatty acid assembly enzymes either native to the organism or expressed through additional transgenic modification.

公开(公告)号：US12104160B2

公开(公告)日：2024-10-01

申请号：US17307850

申请日：2021-05-04

Applicant: ZYMOCHEM, INC.

Inventor： Harshal Chokhawala

IPC: C12P7/42 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/24 ,  C12N9/88 ,  C12N15/52 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/00 ,  C12P17/08 ,  C12P17/10

CPC classification number: C12N15/52 ,  C12N9/00 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/88 ,  C12N9/93 ,  C12P7/04 ,  C12P7/18 ,  C12P7/24 ,  C12P7/40 ,  C12P7/42 ,  C12P7/44 ,  C12P7/6409 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12P17/10 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01035 ,  C12Y101/01078 ,  C12Y101/01268 ,  C12Y101/01269 ,  C12Y102/01003 ,  C12Y102/01005 ,  C12Y102/01022 ,  C12Y102/01024 ,  C12Y102/01026 ,  C12Y102/01048 ,  C12Y102/01063 ,  C12Y102/07005 ,  C12Y102/99006 ,  C12Y103/01044 ,  C12Y103/01045 ,  C12Y103/01086 ,  C12Y104/01 ,  C12Y203/01001 ,  C12Y203/01032 ,  C12Y203/01035 ,  C12Y203/01057 ,  C12Y206/01008 ,  C12Y206/01009 ,  C12Y206/01036 ,  C12Y206/01043 ,  C12Y206/01048 ,  C12Y206/01076 ,  C12Y206/01082 ,  C12Y207/01031 ,  C12Y207/01165 ,  C12Y208/03 ,  C12Y301/03 ,  C12Y301/03002 ,  C12Y301/03008 ,  C12Y301/03019 ,  C12Y301/0302 ,  C12Y302/01 ,  C12Y401/01001 ,  C12Y401/02 ,  C12Y401/02014 ,  C12Y401/0202 ,  C12Y401/02021 ,  C12Y401/03016 ,  C12Y401/03017 ,  C12Y402/01002 ,  C12Y402/01003 ,  C12Y402/01028 ,  C12Y402/0103 ,  C12Y402/01079 ,  C12Y402/0112 ,  C12Y602/01 ,  Y02E50/30

Abstract: Provided herein are methods, compositions, and non-naturally occurring microbial organism for preparing compounds such as1-butanol, butyric acid, succinic acid, 1,4-butanediol, 1-pentanol, pentanoic acid, glutaric acid, 1,5-pentanediol, 1-hexanol, hexanoic acid, adipic acid, 1,6-hexanediol, 6-hydroxy hexanoic acid, ε-Caprolactone, 6-amino-hexanoic acid, ε-Caprolactam, hexamethylenediamine, linear fatty acids and linear fatty alcohols that are between 7-25 carbons long, linear alkanes and linear α-alkenes that are between 6-24 carbons long, sebacic acid and dodecanedioic acid comprising: a) converting a CN aldehyde and pyruvate to a CN+3 β-hydroxyketone intermediate through an aldol addition; and b) converting the CN+3 β-hydroxyketone intermediate to the compounds through enzymatic steps, or a combination of enzymatic and chemical steps.

公开(公告)号：US20240309416A1

公开(公告)日：2024-09-19

申请号：US18576640

申请日：2022-10-12

Applicant: SYNAURA BIOTECHNOLOGY (SHANGHAI) CO., LTD.

Inventor： Yan WU ,  Jing TANG ,  Shu WANG ,  Zhenhua TIAN ,  Fei YAO ,  Miao LI ,  Hong XU ,  Chenxi HUANG ,  Yurou LIU

IPC: C12P19/26 ,  C12N1/20 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/90 ,  C12N15/70 ,  C12P19/18 ,  C12R1/19

CPC classification number: C12P19/26 ,  C12N1/205 ,  C12N9/1029 ,  C12N9/1081 ,  C12N9/1085 ,  C12N9/1096 ,  C12N9/1241 ,  C12N9/1294 ,  C12N9/16 ,  C12N9/90 ,  C12N15/70 ,  C12P19/18 ,  C12R2001/19 ,  C12Y203/01004 ,  C12Y204/99001 ,  C12Y204/99004 ,  C12Y205/01056 ,  C12Y206/01016 ,  C12Y207/07043 ,  C12Y207/09002 ,  C12Y301/03 ,  C12Y501/03014

Abstract: The invention discloses a genetically engineered bacterium and its application in the preparation of sialyllactose. The genetically engineered bacterium has an N-acetylneuraminic acid biosynthesis pathway, includes multiple copies of a gene neuB for encoding sialic acid synthase, and the gene neuB is initiated for expression by a strong promoter. Using the genetically engineered bacteria of the invention to produce sialyllactose has the advantages of high yield and low overall cost.

公开(公告)号：US12077787B2

公开(公告)日：2024-09-03

申请号：US17469899

申请日：2021-09-09

Applicant: Haiqin Chen ,  Wei Chen ,  Xin Tang ,  Jun Cao ,  Jianxin Zhao ,  Yongquan Chen ,  Hao Zhang

Inventor： Haiqin Chen ,  Wei Chen ,  Xin Tang ,  Jun Cao ,  Jianxin Zhao ,  Yongquan Chen ,  Hao Zhang

IPC: C12N9/10 ,  C12P7/6445

CPC classification number: C12N9/1029 ,  C12P7/6445 ,  C12Y203/0102

Abstract: Disclosed is a diacylglycerol acyltransferase 1, a recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1, and application thereof in production of triacylglycerol. The diacylglycerol acyltransferase 1 of the invention has a function of catalyzing synthesis of triacylglycerol. After the recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1 of the invention is subjected to induction culture for 48 h, the content of total fatty acid and triacylglycerol in the recombinant Saccharomyces cerevisiae containing the diacylglycerol acyltransferase 1 can be respectively increased by 1.94 folds and 12.09 folds as compared with those of Saccharomyces cerevisiae without the recombinant diacylglycerol acyltransferase 1. The instant invention provides a method for improving the ability of microorganisms to produce polyunsaturated fatty acids (PUFAs) by means of genetic engineering.

公开(公告)号：US20240287553A1

公开(公告)日：2024-08-29

申请号：US18492600

申请日：2023-10-23

Applicant: ISCA Technologies, Inc. ,  SEMIOPLANT AB

Inventor： Jan Christer Löfstedt ,  Per Fredrik Hofvander ,  Binu Antony ,  Honglei Wang ,  Baojian Ding ,  Agenor Mafra-Neto

IPC: C12P7/62 ,  C12N9/02 ,  C12N9/10 ,  C12N15/81

CPC classification number: C12P7/62 ,  C12N9/0008 ,  C12N9/0071 ,  C12N9/1029 ,  C12N15/81

Abstract: ZETA can be produced by genetically modifying a genome to incorporate a gene encoding an E12 fatty acyl desaturase and then providing a fatty acid. The gene encoding an E12 fatty acyl desaturase can be incorporated into a yeast or plant genome.

公开(公告)号：US20240279694A1

公开(公告)日：2024-08-22

申请号：US18582231

申请日：2024-02-20

Applicant: BIOMEDICAN, INC.

Inventor： Maxim Mikheev ,  Difeng Gao

IPC: C12P17/06 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88

CPC classification number: C12P17/06 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/93 ,  C12Y101/01034 ,  C12Y121/03007 ,  C12Y121/03008 ,  C12Y203/0102 ,  C12Y203/01206 ,  C12Y404/01026 ,  C12Y604/01002

Abstract: The present disclosure relates to the production of cannabinoids in yeast. In as aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, one or more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dry weight of fatty acids or fats.

公开(公告)号：US12060592B2

公开(公告)日：2024-08-13

申请号：US17089498

申请日：2020-11-04

Applicant: Codexis Inc.

Inventor： Steven J. Collier ,  Ee Ling Teo ,  Joly Sukumaran ,  Robert J. Wilson ,  Junye Xu

IPC: C12N9/10 ,  C07K14/00 ,  C12N15/52 ,  C12P7/62 ,  C12P17/06

CPC classification number: C12P17/06 ,  C07K14/00 ,  C12N9/1025 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/62 ,  C12Y203/01 ,  Y02P20/52

Abstract: Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented.