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公开(公告)号：US08546125B2
公开(公告)日：2013-10-01
申请号：US13031350
申请日：2011-02-21
申请人： Guo-Qiang Chen , Zheng-Jun Li , Zhen-Yu Shi , Ying-Ying Guo , Jia Jian , Qiong Wu
发明人： Guo-Qiang Chen , Zheng-Jun Li , Zhen-Yu Shi , Ying-Ying Guo , Jia Jian , Qiong Wu
CPC分类号： C12N9/88 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12P7/625 , C12Y101/01036 , C12Y101/01061 , C12Y102/01016 , C12Y203/01009 , C12Y203/01016 , C12Y401/01001
摘要： Known attempts using engineered bacteria to produce P(3HB-co-4HB) with carbon sources that are structurally unrelated to 4-hydroxybutyrate resulted in relatively low 4HB monomer content of 1.5 to 5 mol %. The current invention provides recombinant hosts for producing P(3HB-co-4HB) wherein the plasmid including succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene) further includes pyruvate decarboxylase promoter (Ppdc). It was found that the 4HB monomer content in P(3HB-co-4HB) is significantly enhanced to be over 20 mol %, in the range of 8.8 to 23 mol %.
摘要翻译： 使用工程细菌产生具有与4-羟基丁酸盐结构无关的碳源的P（3HB-co-4HB）的已知尝试导致相对较低的4HB单体含量为1.5至5mol％。 本发明提供了用于产生P（3HB-co-4HB）的重组宿主，其中包含琥珀酸半醛脱氢酶基因（sucD基因）和4-羟基丁酸脱氢酶基因（4hbD基因）的质粒还包括丙酮酸脱羧酶启动子（Ppdc）。 发现P（3HB-co-4HB）中的4HB单体含量显着提高到超过20mol％，在8.8至23mol％的范围内。
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公开(公告)号：US20120214213A1
公开(公告)日：2012-08-23
申请号：US13031350
申请日：2011-02-21
申请人： Guo-Qiang Chen , Zheng-Jun Li , Ying-Ying Guo , Wei-Chuan Lu , Zi-Zhen Zhou
发明人： Guo-Qiang Chen , Zheng-Jun Li , Ying-Ying Guo , Wei-Chuan Lu , Zi-Zhen Zhou
CPC分类号： C12N9/88 , C12N9/0006 , C12N9/0008 , C12N9/1029 , C12P7/625 , C12Y101/01036 , C12Y101/01061 , C12Y102/01016 , C12Y203/01009 , C12Y203/01016 , C12Y401/01001
摘要： Known attempts using engineered bacteria to produce P(3HB-co-4HB) with carbon sources that are structurally unrelated to 4-hydroxybutyrate resulted in relatively low 4HB monomer content of 1.5 to 5 mol %. The current invention provides recombinant hosts for producing P(3HB-co-4HB) wherein the plasmid including succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene) further includes pyruvate decarboxylase promoter (Ppdc). It was found that the 4HB monomer content in P(3HB-co-4HB) is significantly enhanced to be over 20 mol %, in the range of 8.8 to 23 mol %.
摘要翻译： 使用工程细菌产生具有与4-羟基丁酸盐结构无关的碳源的P（3HB-co-4HB）的已知尝试导致相对较低的4HB单体含量为1.5至5mol％。 本发明提供了用于产生P（3HB-co-4HB）的重组宿主，其中包含琥珀酸半醛脱氢酶基因（sucD基因）和4-羟基丁酸脱氢酶基因（4hbD基因）的质粒还包括丙酮酸脱羧酶启动子（Ppdc）。 发现P（3HB-co-4HB）中的4HB单体含量显着提高到超过20mol％，在8.8至23mol％的范围内。
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公开(公告)号：US20050287653A1
公开(公告)日：2005-12-29
申请号：US11169448
申请日：2005-06-29
CPC分类号： C08G63/89 , B01D11/0288 , C08G63/06 , C08G63/90
摘要： Processes for the extraction of PHAs from biomass, said processes comprising: combining the biomass containing the PHAs with a single main solvent to form a biomass liquor; heating the biomass liquor to at least partially solubilize the PHAs from the biomass to form a PHA liquor; separating the biomass from the PHA liquor to form a PHA-enriched liquor; evaporating from 0% to about 50% of the single main solvent from the PHA-enriched liquor to form a solvent vapor and a concentrated PHA-enriched liquor; and cooling the concentrated PHA-enriched liquor to form precipitated PHAs and an impure solvent liquor. Optionally, further recovering the precipitated PHAs from the impure solvent liquor by filtration under pressure. A PHA-enriched liquor and a concentrated PHA-enriched liquor.
摘要翻译： 用于从生物质提取PHA的方法，所述方法包括：将含有PHAs的生物质与单一主要溶剂组合以形成生物质液体; 加热所述生物质液体以使所述PHA至少部分地从所述生物质中溶解以形成PHA液体; 将生物质与PHA液分离以形成富含PHA的液体; 从富含PHA的液体中蒸发单一主要溶剂的0％至约50％以形成溶剂蒸气和浓缩的富含PHA的液体; 并冷却浓缩的富含PHA的液体以形成沉淀的PHA和不纯的溶剂液体。 任选地，在压力下通过过滤进一步从不纯的溶剂液中回收沉淀的PHA。 富含PHA的液体和浓缩的富含PHA的液体。
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公开(公告)号：US07226765B2
公开(公告)日：2007-06-05
申请号：US11169448
申请日：2005-06-29
申请人： Karunakaran Narasimhan , Kenneth Yee , Angella Christine Cearley , Donald Levengood , Guo-Qiang Chen
发明人： Karunakaran Narasimhan , Kenneth Yee , Angella Christine Cearley , Donald Levengood , Guo-Qiang Chen
CPC分类号： C08G63/89 , B01D11/0288 , C08G63/06 , C08G63/90
摘要： Processes for the extraction of PHAs from biomass, said processes comprising: combining the biomass containing the PHAs with a single main solvent to form a biomass liquor; heating the biomass liquor to at least partially solubilize the PHAs from the biomass to form a PHA liquor; separating the biomass from the PHA liquor to form a PHA-enriched liquor; evaporating from 0% to about 50% of the single main solvent from the PHA-enriched liquor to form a solvent vapor and a concentrated PHA-enriched liquor; and cooling the concentrated PHA-enriched liquor to form precipitated PHAs and an impure solvent liquor. Optionally, further recovering the precipitated PHAs from the impure solvent liquor by filtration under pressure. A PHA-enriched liquor and a concentrated PHA-enriched liquor.
摘要翻译： 用于从生物质提取PHA的方法，所述方法包括：将含有PHAs的生物质与单一主要溶剂组合以形成生物质液体; 加热所述生物质液体以使所述PHA至少部分地从所述生物质中溶解以形成PHA液体; 将生物质与PHA液分离以形成富含PHA的液体; 从富含PHA的液体中蒸发单一主要溶剂的0％至约50％以形成溶剂蒸气和浓缩的富含PHA的液体; 并冷却浓缩的富含PHA的液体以形成沉淀的PHA和不纯的溶剂液体。 任选地，在压力下通过过滤进一步从不纯的溶剂液中回收沉淀的PHA。 富含PHA的液体和浓缩的富含PHA的液体。
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