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1.发明申请CHOLESTEROL LOADED INSECT CELL MEMBRANES AS TEST SYSTEMS FOR ABC TRANSPORTER PROTEINS 有权胆固醇负载细胞膜作为ABC运输蛋白的测试系统公开(公告)号：US20100021927A1
公开(公告)日：2010-01-28
申请号：US12300479
申请日：2007-05-14
申请人： György Báthori , Dóra Méhn , Pál Ákos , Péter Krajcsi , Lajos Szente , Éva Fenyvesi , Ágnes Telbisz , Balázs Sarkadi , András Váradi , Szilvia Gedey , Hristos Glavinas , Emese Kis , Tünde Nagy , Attila Németh , Éva Molnár
发明人： György Báthori , Dóra Méhn , Pál Ákos , Péter Krajcsi , Lajos Szente , Éva Fenyvesi , Ágnes Telbisz , Balázs Sarkadi , András Váradi , Szilvia Gedey , Hristos Glavinas , Emese Kis , Tünde Nagy , Attila Németh , Éva Molnár
CPC分类号： G01N33/6872 , G01N33/92
摘要： The invention provides for a novel cholesterol loaded insect cell membrane preparation having an increased cholesterol level as compared to physiological cholesterol levels of insect cell membranes or to control insect cell membrane preparations without cholesterol loading, wherein said cholesterol loaded membrane preparation comprises an ABC transporter protein having an increased substrate transport activity due to increased cholesterol level of the membrane. The invention also relates to reagent kits comprising the preparations of the invention. The invention also relates to methods for manufacturing said preparations and methods for measuring any type of activity of the ABC transporters present in the cholesterol loaded membranes as well as studying or testing compounds and interaction of compounds and ABC transporters, in this assay systems. The invention also provides for a test system useful for testing whether ABC transporter proteins can be activated by cholesterol in an insect cell membrane.
摘要翻译： 本发明提供了与昆虫细胞膜的生理胆固醇水平相比具有增加的胆固醇水平或控制没有胆固醇负荷的昆虫细胞膜制剂的新型胆固醇负载的昆虫细胞膜制剂，其中所述胆固醇负载的膜制剂包含具有 由于膜的胆固醇水平升高导致的底物转运活性增加。 本发明还涉及包含本发明制剂的试剂盒。 本发明还涉及用于制备所述制剂的方法和用于测量存在于胆固醇负载膜中的ABC转运蛋白的任何类型的活性的方法，以及在该测定系统中研究或测试化合物和化合物和ABC转运蛋白的相互作用。 本发明还提供了一种用于测试ABC转运蛋白是否可以在昆虫细胞膜中被胆固醇激活的测试系统。
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公开(公告)号：US08129197B2
公开(公告)日：2012-03-06
申请号：US12300479
申请日：2007-05-14
申请人： György Báthori , Dóra Méhn , Pál Ákos , Péter Krajcsi , Lajos Szente , Éva Fenyvesi , Ágnes Telbisz , Balázs Sarkadi , András Váradi , Szilvia Gedey , Hristos Glavinas , Emese Kis , Tünde Nagy , Attila Németh , Éva Molnár
发明人： György Báthori , Dóra Méhn , Pál Ákos , Péter Krajcsi , Lajos Szente , Éva Fenyvesi , Ágnes Telbisz , Balázs Sarkadi , András Váradi , Szilvia Gedey , Hristos Glavinas , Emese Kis , Tünde Nagy , Attila Németh , Éva Molnár
CPC分类号： G01N33/6872 , G01N33/92
摘要： The invention provides for a novel cholesterol loaded insect cell membrane preparation having an increased cholesterol level as compared to physiological cholesterol levels of insect cell membranes or to control insect cell membrane preparations without cholesterol loading, wherein said cholesterol loaded membrane preparation comprises an ABC transporter protein having an increased substrate transport activity due to increased cholesterol level of the membrane. The invention also relates to reagent kits comprising the preparations of the invention. The invention also relates to methods for manufacturing said preparations and methods for measuring any type of activity of the ABC transporters present in the cholesterol loaded membranes as well as studying or testing compounds and interaction of compounds and ABC transporters, in this assay systems. The invention also provides for a test system useful for testing whether ABC transporter proteins can be activated by cholesterol in an insect cell membrane.
摘要翻译： 本发明提供了与昆虫细胞膜的生理胆固醇水平相比具有增加的胆固醇水平或控制没有胆固醇负荷的昆虫细胞膜制剂的新型胆固醇负载的昆虫细胞膜制剂，其中所述胆固醇负载的膜制剂包含具有 由于膜的胆固醇水平升高导致的底物转运活性增加。 本发明还涉及包含本发明制剂的试剂盒。 本发明还涉及用于制备所述制剂的方法和用于测量存在于胆固醇负载膜中的ABC转运蛋白的任何类型的活性的方法，以及在该测定系统中研究或测试化合物和化合物和ABC转运蛋白的相互作用。 本发明还提供了一种用于测试ABC转运蛋白是否可以在昆虫细胞膜中被胆固醇激活的测试系统。
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