公开(公告)号：US09512469B2

公开(公告)日：2016-12-06

申请号：US14347855

申请日：2012-09-26

Applicant: HTG Molecular Diagnostics, Inc.

Inventor： Bruce Seligmann ,  Matt Rounseville ,  Krishna Maddula ,  Ihab Botros ,  Chris Cox

IPC: C12Q1/68 ,  C12M1/34 ,  C12N9/22 ,  C07H21/02 ,  C07H21/04 ,  G01N31/22 ,  C40B30/04

CPC classification number: C12Q1/6816 ,  C12Q2521/325 ,  C12Q2525/207 ,  C12Q2537/143 ,  C12Q2561/108

Abstract: Disclosed herein are methods of co-detecting presence of target messenger RNA (mRNA) and small non-coding RNA (for example, miRNA) in a sample. The disclosed methods can be used to simultaneously detect mRNA and small non-coding RNA in a single assay (for example in the same reaction or the same well of a multi-well assay). The methods can include contacting a sample with a plurality of nuclease protection probes (NPPs) including at least one probe which specifically binds to a target mRNA and at least one probe which specifically binds to a target small non-coding RNA, contacting the sample with a nuclease specific for single-stranded nucleic acids, and detecting the NPP, for example on a microarray.

Abstract translation: 本文公开了共检测样品中靶信使RNA（mRNA）和小非编码RNA（例如miRNA）的存在的方法。 所公开的方法可以用于在单个测定中（例如在多孔测定的相同反应或相同的孔中）同时检测mRNA和小的非编码RNA。 所述方法可以包括使样品与多个核酸酶保护探针（NPP）接触，所述核酸酶保护探针（NPP）包括至少一个特异性结合靶mRNA的探针和至少一个特异性结合目标小非编码RNA的探针，使样品与 对单链核酸特异的核酸酶，并且例如在微阵列上检测NPP。

公开(公告)号：US20140243238A1

公开(公告)日：2014-08-28

申请号：US14347855

申请日：2012-09-26

Applicant: HTG MOLECULAR DIAGNOSTICS, INC.

Inventor： Bruce A. Seligmann ,  Matt Rounseville ,  Krishna Maddula ,  Ihab Botros ,  Chris Cox

IPC: C12Q1/68

CPC classification number: C12Q1/6816 ,  C12Q2521/325 ,  C12Q2525/207 ,  C12Q2537/143 ,  C12Q2561/108

Abstract: Disclosed herein are methods of co-detecting presence of target messenger RNA (mRNA) and small non-coding RNA (for example, miRNA) in a sample. The disclosed methods can be used to simultaneously detect mRNA and small non-coding RNA in a single assay (for example in the same reaction or the same well of a multi-well assay). The methods can include contacting a sample with a plurality of nuclease protection probes including at least one probe which specifically binds to a target mRNA and at least one probe which specifically binds to a target small non-coding RNA, contacting the sample with a nuclease specific for single-stranded nucleic acids, and detecting the NPP, for example on a microarray.

Abstract translation: 本文公开了共检测样品中靶信使RNA（mRNA）和小非编码RNA（例如miRNA）的存在的方法。 所公开的方法可以用于在单个测定中（例如在多孔测定的相同反应或相同的孔中）同时检测mRNA和小的非编码RNA。 所述方法可以包括使样品与多个核酸酶保护探针接触，所述核酸酶保护探针包括至少一个特异性结合靶mRNA的探针和至少一个特异性结合靶小编码RNA的探针，使样品与核酸酶特异性接触 对于单链核酸，并且例如在微阵列上检测NPP。