公开(公告)号：US20180340231A1

公开(公告)日：2018-11-29

申请号：US15756641

申请日：2016-09-29

申请人： HTG Molecular Diagnostics, Inc.

发明人： Bonnie LaFleur ,  Qian Liu ,  John W. Luecke ,  Patrick C. Roche

IPC分类号： C12Q1/6886

摘要： Described herein are innovations for classifying subtypes of DLBCL, as well as using the results of classification for diagnosis, prognosis, and therapy selection. In this way, the classifier can effectively classify subtypes of DLBCL and provide meaningful output for the benefit of medical practices and DLBCL patients. Also described are arrays and kits that can be used to measure expression of DLBCL signatures genes.

公开(公告)号：US20160265036A1

公开(公告)日：2016-09-15

申请号：US15034831

申请日：2014-11-05

申请人： HTG MOLECULAR DIAGNOSTICS, INC.

发明人： Matt Rounseville ,  Vijay Modur

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q1/6834 ,  C12Q2521/327 ,  C12Q2521/501 ,  C12Q2521/514 ,  C12Q2525/161 ,  C12Q2533/107 ,  C12Q2537/125 ,  C12Q2561/125 ,  C12Q2565/519 ,  C12Q2521/319

摘要： Disclosed herein are methods for detecting a target nucleic acid molecule in a sample. The methods can include contacting a sample with a detectably labeled probe (detection probe) that specifically binds to a first target sequence in the target nucleic acid molecule, a bifunctional oligonucleotide including a portion that specifically binds to a second target sequence in the target nucleic acid molecule and a portion that specifically binds to an anchor, and a surface comprising the anchor. Specifically bound detection probe and bifunctional oligonucleotide are ligated and a reagent that specifically removes substantially all of the target nucleic acid is added. Unligated detection probe is removed and presence of the detectable label is detected. In other embodiments, the ligation and/or removal of target nucleic acid are omitted and the detection probe specifically bound to the target nucleic acid is detected.

摘要翻译： 本文公开了用于检测样品中靶核酸分子的方法。 所述方法可以包括将样品与特异性结合靶核酸分子中的第一靶序列的可检测标记的探针（检测探针）接触，双功能寡核苷酸，包括特异性结合靶核酸中的第二靶序列的部分 分子和特异性结合锚的部分，以及包含锚的表面。 连接特异性结合的检测探针和双功能寡核苷酸，并添加特异性去除基本上所有靶核酸的试剂。 去除未螯合的检测探针，检测到可检测标记的存在。 在其它实施方案中，省略靶核酸的连接和/或去除，并检测特异性结合靶核酸的检测探针。

公开(公告)号：US12104206B2

公开(公告)日：2024-10-01

申请号：US16070678

申请日：2017-02-10

申请人： HTG Molecular Diagnostics, Inc.

发明人： Debrah Thompson ,  Matthew Rounseville

IPC分类号： C12Q1/6806 ,  C12Q1/6869

CPC分类号： C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6806 ,  C12Q2521/325 ,  C12Q2521/501 ,  C12Q2521/531 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/191 ,  C12Q2525/207 ,  C12Q2533/107 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/162 ,  C12Q2563/131 ,  C12Q2563/149 ,  C12Q1/6869 ,  C12Q2521/325 ,  C12Q2521/501 ,  C12Q2521/531 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/191 ,  C12Q2525/207 ,  C12Q2533/107 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/162 ,  C12Q2563/131 ,  C12Q2563/149

摘要： The present disclosure provides methods and kits for direct sequencing of nucleic acid targets. Such methods can be used to determine if one or more nucleic acid targets are present in a sample.

公开(公告)号：US20160019337A1

公开(公告)日：2016-01-21

申请号：US14772038

申请日：2014-03-03

申请人： HTG MOLECULAR DIAGNOSTICS, INC.

发明人： Christopher Roberts ,  Hui Wang ,  Zhenquiang Lu ,  Krishna Maddula ,  Sam Rua ,  Kevin Knapp ,  Byron Lawson ,  Debrah Thompson ,  Michael Hrubiak ,  Tyler Breedlove ,  Vijay Modur

IPC分类号： G06F19/20 ,  C12Q1/68

CPC分类号： G16B25/00 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/57423

摘要： This disclosure concerns the identification of biomarkers that are characteristic of squamous or non squamous (e.g., adenocarcinoma, large cell carcinoma, carcinoid tumor, sarcomatoid carcinoma) subtypes of non small cell lung cancer (NSCLC), clinically useful NSCLC classifiers, kits and arrays for distinguishing squamous and nonsquamous NSCLC subtypes, bioinformatic methods for determining clinically useful classifiers, and methods of use of each of the foregoing.

摘要翻译： 本公开涉及鉴定非小细胞肺癌（NSCLC）的鳞状或非鳞状体（例如，腺癌，大细胞癌，类癌瘤，肉瘤样癌）亚型，临床上有用的NSCLC分类器，试剂盒和阵列的特征性生物标志物 区分鳞状和非鳞状NSCLC亚型，用于确定临床有用的分类器的生物信息学方法，以及上述每一种的使用方法。

公开(公告)号：US20140235460A1

公开(公告)日：2014-08-21

申请号：US14266884

申请日：2014-05-01

申请人： HTG Molecular Diagnostics, Inc.

发明人： Debrah Thompson ,  Bruce E. Seligmann ,  Debra A. Gordon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2561/108

摘要： The present disclosure provides an improvement to quantitative Nuclease Protection Assay (qNPA) and quantitative Nuclease Protection Sequencing (qNPS) methods. The disclosed methods use nuclease protection probes (NPPs) that include 5′-end and/or 3-end flanking sequences, which provide a universal hybridization and/or amplification sequence. The disclosed methods can be used to sequence or detect target nucleic acid molecules, such as those present in fixed or insoluble samples.

摘要翻译： 本公开提供了定量核酸酶保护测定（qNPA）和定量核酸酶保护测序（qNPS）方法的改进。 所公开的方法使用包括5'端和/或3端侧翼序列的核酸酶保护探针（NPP），其提供通用杂交和/或扩增序列。 所公开的方法可用于序列或检测靶核酸分子，例如存在于固定或不溶性样品中的核酸分子。

公开(公告)号：US20220106640A1

公开(公告)日：2022-04-07

申请号：US17416982

申请日：2019-12-02

申请人： HTG Molecular Diagnostics, Inc.

发明人： Debrah Thompson ,  Matthew Rounseville

IPC分类号： C12Q1/6874 ,  C12Q1/6853

摘要： The present disclosure provides methods for sequencing nucleic acid targets (e.g., both DNA and RNA co-amplified in a sample mixture, for example by using a surrogate for the RNA). Such methods can be used to determine if one or more nucleic acid targets are present in a sample.

公开(公告)号：US11268133B2

公开(公告)日：2022-03-08

申请号：US16377650

申请日：2019-04-08

申请人： HTG Molecular Diagnostics, Inc.

发明人： Bruce A. Seligmann ,  BJ Kerns ,  John Luecke ,  Matt Rounseville ,  Ihab Botros ,  Mark Schwartz

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6886

摘要： Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.

公开(公告)号：US09765385B2

公开(公告)日：2017-09-19

申请号：US14411806

申请日：2013-06-28

申请人： HTG Molecular Diagnostics, Inc.

发明人： Matt Rounseville ,  Bruce Seligmann

IPC分类号： C12Q1/68

CPC分类号： C12Q1/683 ,  C12Q1/6827 ,  C12Q2521/325 ,  C12Q2561/108 ,  C12Q2537/143 ,  C12Q2563/107

摘要： Disclosed herein are methods for detecting presence of a nucleotide variant in a target nucleic acid utilizing a nuclease protection assay. The methods include contacting a sample with at least two probes, wherein the first probe is complementary to the wild-type (non-variant) nucleotide(s) at the nucleotide variant position(s) in the target nucleic acid and the second probe is complementary to the variant nucleotide(s) at the nucleotide variant position(s) in the target nucleic acid, under conditions sufficient for the probes to hybridize to the target nucleic acid, producing a mixture of hybridized and unhybridized nucleic acids. The mixture is contacted with a nuclease specific for single-stranded nucleic acid molecules under conditions sufficient to remove unhybridized nucleic acid molecules (or unhybridized portions of nucleic acid molecules). The presence of the at least two probes is then detected, thereby detecting the presence of the variant and/or non-variant target nucleic acid in the sample.

公开(公告)号：US09758829B2

公开(公告)日：2017-09-12

申请号：US14405739

申请日：2013-06-24

申请人： HTG Molecular Diagnostics, Inc. ,  John Wayne Cancer Institute

发明人： Hui Wang ,  Christopher Roberts ,  Krishna Maddula ,  Zhenquiang Lu ,  Tom Vasicek ,  B J Kerns ,  Bruce E. Seligmann ,  Dave S. B. Hoon

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/166 ,  C12Q2600/178

摘要： Disclosed are methods for determining whether a melanocyte-containing sample (such as a nevus or other pigmented lesion) is benign or a primary melanoma. These methods can include detecting (at the molecular level, e.g., mRNA, miRNA, or protein) the expression of at least two disclosed genes in a biological sample obtained from a subject. Also provided are arrays and kits that can be used with the methods.

公开(公告)号：US20150191770A1

公开(公告)日：2015-07-09

申请号：US14411806

申请日：2013-06-28

申请人： HTG Molecular Diagnostics, Inc.

发明人： Matt Rounseville ,  Bruce Seligmann

IPC分类号： C12Q1/68

CPC分类号： C12Q1/683 ,  C12Q1/6827 ,  C12Q2521/325 ,  C12Q2561/108 ,  C12Q2537/143 ,  C12Q2563/107

摘要： Disclosed herein are methods for detecting presence of a nucleotide variant in a target nucleic acid utilizing a nuclease protection assay. The methods include contacting a sample with at least two probes, wherein the first probe is complementary to the wild-type (non-variant) nucleotide(s) at the nucleotide variant position(s) in the target nucleic acid and the second probe is complementary to the variant nucleotide(s) at the nucleotide variant position(s) in the target nucleic acid, under conditions sufficient for the probes to hybridize to the target nucleic acid, producing a mixture of hybridized and unhybridized nucleic acids. The mixture is contacted with a nuclease specific for single-stranded nucleic acid molecules under conditions sufficient to remove unhybridized nucleic acid molecules (or unhybridized portions of nucleic acid molecules). The presence of the at least two probes is then detected, thereby detecting the presence of the variant and/or non-variant target nucleic acid in the sample.

摘要翻译： 本文公开了使用核酸酶保护测定法检测靶核酸中核苷酸变体的存在的方法。 所述方法包括使样品与至少两个探针接触，其中第一探针与靶核酸中核苷酸变体位置处的野生型（非变体）核苷酸互补，第二探针是 与靶核酸的核苷酸变体位置的变体核苷酸互补，在足以使探针与靶核酸杂交的条件下，产生杂交和未杂交的核酸的混合物。 在足以除去未杂交的核酸分子（或核酸分子的未杂交部分）的条件下，将混合物与单链核酸分子特异性核酸酶接触。 然后检测至少两个探针的存在，从而检测样品中变体和/或非变体靶核酸的存在。