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公开(公告)号：US08228499B2
公开(公告)日：2012-07-24
申请号：US12682234
申请日：2008-09-18
CPC分类号： G02B21/34
摘要： The invention relates to methods for positioning at least one preferably biological specimen in the specimen space of a microscope arrangement, and to devices for carrying out these methods. Methods and devices are proposed, wherein the specimen's orientation relative to a detection objective's optical axis can be repeatedly changed and, in doing so, the specimen is held so that a substantially unobstructed view of the specimen is ensured from every detection direction. In different constructional variants, the specimen is held at a supporting device by adhesive forces or by a flowing medium, the specimen is held at a capillary opening by capillary action, or at least one specimen is embedded in a body of transparent gel, and the gel body is fixed in the specimen space by means of a rotatable holding device, and the detection direction is changed by rotating the holding device by a given angle of rotation.
摘要翻译： 本发明涉及将显影装置的样本空间中至少一个优选生物样本定位的方法以及用于实施这些方法的装置。 提出了相对于检测对象的光轴的试样的取向可以反复改变的方法和装置，并且在这样做的同时，保持试样，使得从每个检测方向确保了样本的基本无阻碍的视图。 在不同的结构变型中，试样通过粘合力或流动介质保持在支撑装置上，通过毛细管作用将样品保持在毛细管开口处，或者至少一个样品嵌入透明凝胶体中， 凝胶体通过可旋转保持装置固定在样本空间中，并且通过使保持装置旋转给定的旋转角度来改变检测方向。
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公开(公告)号：US20100177381A1
公开(公告)日：2010-07-15
申请号：US12597144
申请日：2008-04-04
IPC分类号： G02B21/34
CPC分类号： G02B21/26
摘要： The invention relates to a sample holding system for a microscope, comprising a sample chamber which has an upper opening and is filled with an immersion liquid, and in which a sample embedded in a transparent embedding compound is placed in a holder. The sample holding system also comprises means for the translatory movement of the sample in relation to a detection objective of the microscope, and means for rotating the sample about an essentially vertical rotational axis in a plane forming an angle different from zero with the optical axis of the detection objective. The means for rotating the sample in such a sample holding system comprise a rotational drive provided with a magnetic coupling or a belt drive and/or toothed wheel rotational drive arranged above the sample chamber, said drives transmitting the rotational movement to the holder, or the rotational movement is generated directly by the movement of the entire sample chamber.
摘要翻译： 本发明涉及一种用于显微镜的样品保持系统，其包括具有上部开口并填充有浸没液体的样品室，并且将嵌入在透明嵌入物中的样品放置在保持器中。 样品保持系统还包括用于相对于显微镜的检测目标的样品的平移运动的装置，以及用于使样品围绕基本上垂直的旋转轴旋转的装置，该平面形成与零的光轴不同的角度 检测目标。 在这种样品保持系统中旋转样品的装置包括旋转驱动器，其设置有设置在样品室上方的磁耦合或带驱动和/或齿轮旋转驱动器，所述驱动器将旋转运动传递到保持器，或 通过整个样品室的运动直接产生旋转运动。
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公开(公告)号：US08699133B2
公开(公告)日：2014-04-15
申请号：US12597144
申请日：2008-04-04
IPC分类号： G02B21/26
CPC分类号： G02B21/26
摘要： A sample holding system for a microscope, including a sample chamber which has an upper opening and is filled with an immersion liquid, and in which a sample embedded in a transparent embedding compound is placed in a holder. Translatory movement of the sample in relation to a detection objective of the microscope, and rotating the sample about an essentially vertical rotational axis in a plane forming an angle different from zero with the optical axis of the detection objective is included. Rotating the sample includes a rotational drive provided with a magnetic coupling or a belt drive and/or toothed wheel rotational drive arranged above the sample chamber.
摘要翻译： 一种用于显微镜的样品保持系统，包括具有上部开口并填充有浸没液体的样品室，并且将嵌入在透明嵌入物中的样品放置在保持器中。 样品相对于显微镜的检测目标的平移运动，并且包括在与检测对象的光轴形成不同于零的角度的平面中基本上垂直的旋转轴线旋转样品。 旋转样品包括旋转驱动器，其设置有设置在样品室上方的磁耦合或带驱动和/或齿轮旋转驱动。
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公开(公告)号：US20100239138A1
公开(公告)日：2010-09-23
申请号：US12682234
申请日：2008-09-18
CPC分类号： G02B21/34
摘要： The invention relates to methods for positioning at least one preferably biological specimen in the specimen space of a microscope arrangement, and to devices for carrying out these methods. Methods and devices are proposed, wherein the specimen's orientation relative to a detection objective's optical axis can be repeatedly changed and, in doing so, the specimen is held so that a substantially unobstructed view of the specimen is ensured from every detection direction. In different constructional variants, the specimen is held at a supporting device by adhesive forces or by a flowing medium, the specimen is held at a capillary opening by capillary action, or at least one specimen is embedded in a body of transparent gel, and the gel body is fixed in the specimen space by means of a rotatable holding device, and the detection direction is changed by rotating the holding device by a given angle of rotation.
摘要翻译： 本发明涉及将显影装置的样本空间中至少一个优选生物样本定位的方法以及用于实施这些方法的装置。 提出了相对于检测对象的光轴的试样的取向可以反复改变的方法和装置，并且在这样做的同时，保持试样，使得从每个检测方向确保了样本的基本无阻碍的视图。 在不同的结构变型中，试样通过粘合力或流动介质保持在支撑装置上，通过毛细管作用将样品保持在毛细管开口处，或者至少一个样品嵌入透明凝胶体中， 凝胶体通过可旋转保持装置固定在样本空间中，并且通过使保持装置旋转给定的旋转角度来改变检测方向。
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公开(公告)号：US20100193673A1
公开(公告)日：2010-08-05
申请号：US12680165
申请日：2008-09-16
IPC分类号： H05H3/00
CPC分类号： G02B21/32 , G02B21/0032
摘要： The invention is directed to an optical arrangement for photomanipulation of a sample comprising a sample holder for receiving the sample, an illumination device comprising an illumination light source and an illumination beam path for illuminating the sample with a light sheet. It further comprises a detection device for detecting light that is radiated from the sample and imaging optics which image the sample on the detection device by means of an imaging objective in an imaging beam path, wherein the light sheet is substantially planar in the focus of the imaging objective, and wherein the imaging objective has an optical axis which intersects the plane of the light sheet at an angle different from zero. Further, the arrangement also has means for photomanipulation of the sample.
摘要翻译： 本发明涉及用于光调制样品的光学装置，其包括用于接收样品的样品保持器，包括照明光源的照明装置和用于用光照射样品的照明光束路径。 它还包括检测装置，用于检测从样本辐射的光和成像光学元件，其通过成像光束路径中的成像目标将检测装置上的样品成像在检测装置上，其中光片在 成像目标，并且其中所述成像物镜具有与所述光片的平面以不同于零的角度相交的光轴。 此外，该装置还具有用于对样品进行光电处理的装置。
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公开(公告)号：US08482854B2
公开(公告)日：2013-07-09
申请号：US12529051
申请日：2008-03-26
摘要： The invention is directed to a sample holder for a microscope. The sample holder comprises a sample chamber which is filled with an immersion liquid and in which a sample is located. The sample chamber has an upper opening. It further comprises means for translating the sample relative to a detection objective of the microscope, and means for rotating the sample around an axis of rotation extending in a substantially horizontal plane which encloses an angle other than zero degrees with the optical axis of the detection objective. In a sample holder of this kind, the sample is embedded in a transparent embedding medium having at least partially a greater solidity than the immersion liquid. Further, the sample chamber has means for horizontally supporting the embedded sample against the effect of gravity.
摘要翻译： 本发明涉及用于显微镜的样品架。 样品架包括样品室，其中填充有浸没液体并且样品位于样品室中。 样品室具有上部开口。 它还包括用于相对于显微镜的检测对象平移样本的装置，以及用于围绕围绕旋转轴旋转的装置的装置，该旋转轴在基本上水平的平面中旋转，该基本上水平的平面与检测目标的光轴 。 在这种样品保持器中，将样品嵌入至少部分具有比浸没液更大的固体的透明嵌入介质中。 此外，样品室具有用于水平地支撑嵌入样品免受重力影响的装置。
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公开(公告)号：US08213081B2
公开(公告)日：2012-07-03
申请号：US12596412
申请日：2008-04-04
IPC分类号： G02B21/02
CPC分类号： G02B21/248 , G02B21/33
摘要： The invention relates to an objective replacement device for a microscope, wherein the sample is located in a sample chamber and surrounded by an immersion medium within the sample chamber, means for positioning and aligning the sample relative to the focus of an objective being present, wherein the detection beam path is aligned horizontally, which is to say perpendicular to the direction of action of gravity. For an objective replacement device for a microscope of the type described above, according to the invention a device is provided for exchanging the objective, at the focus of which the sample is positioned and aligned, with at least another objective, the position and alignment of the sample within the sample chamber remaining the same.
摘要翻译： 本发明涉及一种用于显微镜的目标替换装置，其中样品位于样品室中并被样品室内的浸没介质包围，用于相对于存在的物镜的焦点定位和对准样品的装置，其中 检测光束路径水平对准，即垂直于重力作用方向。 对于上述类型的显微镜的目标替换装置，根据本发明，提供了一种装置，用于在样品的焦点位置和对准位置处与至少另一个物体交换物镜的位置和对准 样品室内的样品保持不变。
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公开(公告)号：US20110031414A1
公开(公告)日：2011-02-10
申请号：US12937103
申请日：2009-04-04
IPC分类号： G01J1/58
CPC分类号： G02B21/16 , G02B21/002 , G02B21/06 , G02B21/10
摘要： A microscopy device, particularly for use in an imaging fluorescence lifetime microscopy method is provided. The microscopy device comprises an illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined, an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector. The illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path, wherein the axis of the detection beam path is oriented substantially perpendicular to a section plane of the light sheet and of the object to be examined. The illumination means comprise a pulsed laser.
摘要翻译： 提供了特别用于成像荧光寿命显微术方法的显微镜装置。 显微镜装置包括用于产生照明光束的照明装置，用于空间分辨地获取由待检查对象发射的发射辐射的成像检测器，照明装置和待检查对象之间的照射光束路径，以及检测 要检查的物体与检测器之间的光束路径。 照明光束路径包括照明光学器件，其设计成产生横向于照明光束路径的轴线延伸的照明辐射的光片，其中检测光束路径的轴线基本上垂直于光片的截面定向， 的被检查对象。 照明装置包括脉冲激光器。
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公开(公告)号：US20100134881A1
公开(公告)日：2010-06-03
申请号：US12596412
申请日：2008-04-04
IPC分类号： G02B21/02
CPC分类号： G02B21/248 , G02B21/33
摘要： The invention relates to an objective replacement device for a microscope, wherein the sample is located in a sample chamber and surrounded by an immersion medium within the sample chamber, means for positioning and aligning the sample relative to the focus of an objective being present, wherein the detection beam path is aligned horizontally, which is to say perpendicular to the direction of action of gravity. For an objective replacement device for a microscope of the type described above, according to the invention a device is provided for exchanging the objective, at the focus of which the sample is positioned and aligned, with at least another objective, the position and alignment of the sample within the sample chamber remaining the same.
摘要翻译： 本发明涉及一种用于显微镜的目标替换装置，其中样品位于样品室中并被样品室内的浸没介质包围，用于相对于存在的物镜的焦点定位和对准样品的装置，其中 检测光束路径水平对准，即垂直于重力作用方向。 对于上述类型的显微镜的目标替换装置，根据本发明，提供了一种装置，用于在样品的焦点位置和对准位置处与至少另一个物体交换物镜的位置和对准 样品室内的样品保持不变。
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公开(公告)号：US08921809B2
公开(公告)日：2014-12-30
申请号：US12937103
申请日：2009-04-04
CPC分类号： G02B21/16 , G02B21/002 , G02B21/06 , G02B21/10
摘要： A microscopy device, particularly for use in an imaging fluorescence lifetime microscopy method is provided. The microscopy device comprises an illumination means for generating an illumination beam, an imaging detector for spatially resolved acquisition of an emission radiation emitted by an object to be examined, an illumination beam path between the illumination means and the object to be examined, and a detection beam path between the object to be examined and the detector. The illumination beam path comprises illumination optics which are designed to generate a light sheet of illumination radiation extending transverse to the axis of the illumination beam path, wherein the axis of the detection beam path is oriented substantially perpendicular to a section plane of the light sheet and of the object to be examined. The illumination means comprise a pulsed laser.
摘要翻译： 提供了特别用于成像荧光寿命显微术方法的显微镜装置。 显微镜装置包括用于产生照明光束的照明装置，用于空间分辨地获取由待检查对象发射的发射辐射的成像检测器，照明装置和待检查对象之间的照明光束路径，以及检测 要检查的物体与检测器之间的光束路径。 照明光束路径包括照明光学器件，其设计成产生横向于照明光束路径的轴线延伸的照明辐射的光片，其中检测光束路径的轴线基本上垂直于光片的截面定向， 的被检查对象。 照明装置包括脉冲激光器。
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