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公开(公告)号：US4173516A
公开(公告)日：1979-11-06
申请号：US840165
申请日：1977-10-06
摘要： A method of cultivating cells of animal and human tissues includes the steps of introducing the cells into a nutrient solution in which they tend to settle, and maintaining the cells in the solution in a suspended state so as to prevent their settling by introducing an oxygen-containing gas into the solution. An apparatus for carrying out the method includes a container for a nutrient solution, an arrangement for introducing the oxygen-containing gas into the container, and a guide arrangement in the container and operative for guiding the nutrient solution so that the latter performs a circulatory movement in the container when the oxygen-containing gas is introduced into the latter. The guide arrangement may be formed as a tubular member, as a substantially flat partition wall, or as an inner wall of a circumferentially complete tubular container.
摘要翻译： 培养动物和人体组织的细胞的方法包括以下步骤：将细胞导入其倾向于沉降的营养液中，并将细胞保持在悬浮状态，以便通过引入氧 - 含有气体进入溶液。 用于实施该方法的装置包括用于营养液的容器，用于将含氧气体引入容器中的装置，以及容器中的引导装置，并用于引导营养液，使得其进行循环运动 在含氧气体被引入后者的容器中。 引导装置可以形成为管状构件，作为基本平坦的分隔壁，或形成为周向完整的管状容器的内壁。
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2.发明授权
公开(公告)号：US4311798A
公开(公告)日：1982-01-19
申请号：US015282
申请日：1979-02-26
CPC分类号： C12M29/14 , C12M23/34 , Y10S435/818
摘要： A method of cultivating cells of animal and human tissues includes the steps of introducing the cells into a nutrient solution in which they tend to settle, and maintaining the cells in the solution in a suspended state so as to prevent their settling by introducing an oxygen-containing gas to the solution. An apparatus for carrying out the method includes a container for a nutrient solution, an arrangement for introducing the oxygen-containing gas into the container, and a guide arrangement in the container and operative for guiding the nutrient solution so that the latter performs a circulatory movement in the container when the oxygen-containing gas is introduced into the latter. The guide arrangement may be formed as a tubular member, as a substantially flat partition wall, or as an inner wall of a circumferentially complete tubular container.
摘要翻译： 培养动物和人体组织的细胞的方法包括以下步骤：将细胞导入其倾向于沉降的营养液中，并将细胞保持在悬浮状态，以便通过引入氧 - 含有气体到溶液中。 用于实施该方法的装置包括用于营养液的容器，用于将含氧气体引入容器中的装置，以及容器中的引导装置，并用于引导营养液，使得其进行循环运动 在含氧气体被引入后者的容器中。 引导装置可以形成为管状构件，作为基本平坦的分隔壁，或形成为周向完整的管状容器的内壁。
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公开(公告)号：US4259449A
公开(公告)日：1981-03-31
申请号：US936600
申请日：1978-08-23
CPC分类号： C12M29/14 , C12M23/34 , Y10S435/818
摘要： A method of cultivating cells of animal and human tissues includes the steps of introducing the cells into a nutrient solution in which they tend to settle, and maintaining the cells in the solution in a suspended state so as to prevent their settling by introducing an oxygen-containing gas into the solution. An apparatus for carrying out the method includes a container for a nutrient solution, an arrangement for introducing the oxygen-containing gas into the container, and a guide arrangement in the container and operative for guiding the nutrient solution so that the latter performs a circulatory movement in the container when the oxygen-containing gas is introduced into the latter. The guide arrangement may be formed as a tubular member, as a substantially flat partition wall, or as an inner wall of a circumferentially complete tubular container.
摘要翻译： 培养动物和人体组织的细胞的方法包括以下步骤：将细胞导入其倾向于沉降的营养液中，并将细胞保持在悬浮状态，以便通过引入氧 - 含有气体进入溶液。 用于实施该方法的装置包括用于营养液的容器，用于将含氧气体引入容器中的装置，以及容器中的引导装置，并用于引导营养液，使得其进行循环运动 在含氧气体被引入后者的容器中。 引导装置可以形成为管状构件，作为基本平坦的分隔壁，或形成为周向完整的管状容器的内壁。
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公开(公告)号：US5866348A
公开(公告)日：1999-02-02
申请号：US731600
申请日：1996-10-16
申请人： Winfried Scheirer
发明人： Winfried Scheirer
CPC分类号： C12Q1/66 , Y10S435/968 , Y10S530/802
摘要： The present invention provides a method of increasing the duration of detectable photon emission of a luciferase-luciferin reaction. The method provides a luciferase-luciferin reaction in which photon emission can be detected for up to and including eight hours. A method of the present invention can also be used to detect the presence of luciferase in biological samples. The present, invention also provides a composition used in detecting the presence of luciferase in biological samples.
摘要翻译： 本发明提供了增加荧光素酶 - 荧光素反应的可检测光子发射持续时间的方法。 该方法提供荧光素酶 - 荧光素反应，其中可以检测光子发射长达8小时。 本发明的方法也可用于检测生物样品中萤光素酶的存在。 本发明还提供了用于检测生物样品中荧光素酶的存在的组合物。
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公开(公告)号：US5618682A
公开(公告)日：1997-04-08
申请号：US193679
申请日：1994-02-08
申请人： Winfried Scheirer
发明人： Winfried Scheirer
CPC分类号： C12Q1/66 , Y10S435/968 , Y10S530/802
摘要： The present invention provides a method of increasing the duration of detectable photon emission of a luciferase-luciferin reaction. The method provides a luciferase-luciferin reaction in which photon emission can be detected for up to and including eight hours. A method of the present invention can also be used to detect the presence of luciferase in biological samples. The present invention also provides a composition used in detecting the presence of luciferase in biological samples.
摘要翻译： 本发明提供了增加荧光素酶 - 荧光素反应的可检测光子发射持续时间的方法。 该方法提供荧光素酶 - 荧光素反应，其中可以检测光子发射长达8小时。 本发明的方法也可用于检测生物样品中萤光素酶的存在。 本发明还提供了用于检测生物样品中荧光素酶的存在的组合物。
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