公开(公告)号：US12104148B1

公开(公告)日：2024-10-01

申请号：US16875907

申请日：2020-05-15

申请人： Humacyte, Inc.

发明人： Robert Schutte ,  Amanda Elizabeth Basciano ,  Dustin Cashman ,  Joshua McCall ,  Laura E. Niklason ,  Heather L Prichard ,  Justin T. Strader

IPC分类号： C12M3/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/42

CPC分类号： C12M21/08 ,  C12M23/58 ,  C12M29/12 ,  C12M29/14 ,  C12M35/04 ,  C12M41/40

摘要： Embodiments of the present disclosure are directed to apparatuses and methods for a pulsing system which can be configured to provide pressure pulses to a fluid communication tube to/from a bioreactor (for example). Such a system may comprise one or more of, and preferably a plurality of a pulsing motor, a compression plate, one or more fluid communications tubes comprising a biomedia fluid tube configured to direct biomedia fluid into and out of one or more bioreactors, and/or an intra-luminal fluid tube configured to direct intra-luminal fluid through a mandrel tube provided in each bioreactor, and one and/or another of a cam, gearing, and/or actuator configured to repetitively moving the compression plate so as to repetitively compress and release at least a portion of a respective intra-luminal fluid tube for a respective bioreactor to effect a temporary pressure increase (distension or strain) in a respective mandrel-tube according to the predetermined time period.

公开(公告)号：US20240263122A1

公开(公告)日：2024-08-08

申请号：US18368554

申请日：2023-09-14

申请人： National Central University ,  National Defense Medical Center

发明人： Ching-Yun Chen ,  Chih-Hung Wang ,  Hsin-Chien Chen ,  Yu-Hsuan Ting ,  Chun-Yi Peng ,  Yueh-Teng Tsai ,  Sheng-Wen Chang ,  Feng-Yuan Chien

IPC分类号： C12M1/00

CPC分类号： C12M29/04 ,  C12M29/14

摘要： Provided is a bioreactor apparatus including: a liquid storage chamber for storing a liquid containing a first ion; a pump; a culture chamber for accommodating the liquid and cells to be cultured; and an ion-exchange chamber accommodating an ion-exchange substrate containing a second ion. Affinity of the second ion to the ion-exchange substrate is lower than affinity of the first ion to the ion-exchange substrate, or molar concentration of the second ion far outweigh molar concentration of the first ion. The storage chamber, the pump, the culture chamber and the ion-exchange chamber are connected via pipelines to form a closed loop, and the pump is configured to provide pressure to drive flow of the liquid in the closed loop. Also provided are a method for regulating ion concentration by the ion-exchange substrate and a method for cell culture by the bioreactor apparatus.

公开(公告)号：US20240101946A1

公开(公告)日：2024-03-28

申请号：US18368879

申请日：2023-09-15

申请人： Terumo BCT, Inc.

发明人： Mindy M. MILLER ,  Maria KNAUB ,  Ann Marie W. CUNNINGHAM ,  Mark E. JONES

IPC分类号： C12M1/00 ,  C12M1/26 ,  C12N5/0783

CPC分类号： C12M23/58 ,  C12M29/14 ,  C12M29/20 ,  C12M33/12 ,  C12N5/0636 ,  C12N2501/2302

摘要： Cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. The cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.

公开(公告)号：US11883575B2

公开(公告)日：2024-01-30

申请号：US16576484

申请日：2019-09-19

申请人： Fenwal, Inc.

发明人： Christopher J. Wegener ,  Bret M. Olson ,  Alaina Schlinker ,  Steven Binninger ,  Avnie A. Kadakia ,  Kyle Thompson ,  Kyle Wolok ,  Melanie Hamilton

IPC分类号： A61M1/26 ,  A61M1/36 ,  C12M1/00 ,  C12M1/26 ,  C12M1/36 ,  G01N33/543

CPC分类号： A61M1/262 ,  A61M1/265 ,  A61M1/362 ,  A61M1/3618 ,  C12M23/28 ,  C12M29/14 ,  C12M33/14 ,  C12M41/48 ,  C12M47/04 ,  G01N33/54326 ,  G01N33/54366

摘要： A cell processing system includes at least one processor connectable to a source container filled with a biological fluid, the processor including a spinning membrane configured to receive and separate target cells from the biological fluid, the target cells exiting at a first outlet, first and second containers selectively connected to the first outlet; and a magnet. The system also includes a controller configured to operate the spinning membrane to receive biological fluid and to direct the target cells to the first container, to pause to permit magnetic particles to be associated with the target cells in the first container, to operate the spinning membrane to receive the contents of the first container with the magnet applied to the target cells associated with the magnetic particles, to remove or deactivate the magnet, and to transfer the target cells to the second container.

公开(公告)号：US20240002773A1

公开(公告)日：2024-01-04

申请号：US18250480

申请日：2021-11-05

申请人： HOFFMANN-LA ROCHE INC.

发明人： Edgar Ernesto CARRASCO ,  Dieter SCHMIDLI ,  Roger STEINER ,  Joerg VOELKLE ,  Johanna Alina SCHNEIDER

IPC分类号： C12M1/00 ,  C12M1/32 ,  C12M3/00 ,  C12M1/12

CPC分类号： C12M39/00 ,  C12M23/12 ,  C12M23/48 ,  C12M29/14 ,  C12M37/06

摘要： A plate cleaning device (1) for cleaning a clamp plate of a fermenter, wherein the clamp plate has a plurality of receptacles each configured to hold a bioreactor vessel, and at least one conduit with a conduit opening per receptacle. The device comprises a plate seat member (3), a cover member (2), a plurality of valve units (4), a first fluid terminal (71), a second fluid terminal (74) and a control unit (9). The plate seat member (3) is configured to hold the clamp plate (6) in a predefined position and orientation. The cover member (2) is configured to close the plate seat member (3) such that the clamp plate (6) is tightly enclosed by the plate seat member (3) and the cover member (2). The plurality of valve units (4) is configured to be coupled to the cover member (2) such that at least one valve unit (4) is associated to each of the conduit openings (62) of the clamp plate (6). The first fluid terminal (71) is configured to receive a first fluid container and the second fluid terminal (74) to receive a second fluid container. The cover member (2) is equipped with channels each having a first open end and a second open end. Each of the first open ends of the channels of the cover member (2) is positioned to be connected to one of the conduit openings (62) of the clamp plate (6). The valve units (4) are configured to be coupled to the second open ends of the channels of the plate such that all of the at least one conduit openings (62) per receptacle are associated to one valve (4). The first fluid terminal (71) and the second fluid terminal (74) are coupled to each of the valve units (4). The control unit (9) is configured to selectively connect the first fluid terminal (71) and the second fluid terminal (74) to the second open ends of the channels of the cover member (2).

公开(公告)号：US20230365908A1

公开(公告)日：2023-11-16

申请号：US18196932

申请日：2023-05-12

申请人： UNIVERSITY OF UTAH RESEARCH FOUNDATION

发明人： Jungkyu Kim ,  Minju Kim ,  Kanghoon Choi ,  Zachary Ryan Estlack

IPC分类号： C12M1/00 ,  C12M3/06 ,  C12M1/34

CPC分类号： C12M23/26 ,  C12M23/16 ,  C12M29/14 ,  C12M41/40

摘要： Described herein are apparatuses and methods for culturing and monitoring cells in dynamic physiological conditions with combinatorial and biomechanical stimulation. In some embodiments, the apparatuses and methods may comprise one or more cell culture chambers, flexible membranes, pneumatic actuators, microfluidic layers, or hydrogels. In some embodiments, various mechanical stimuli including bending stress, shear stress, or a combination thereof may be applied to one or more cell types. Also described herein are high-throughput and dynamic cell culture array systems comprising the described apparatuses and methods.

公开(公告)号：US11655442B2

公开(公告)日：2023-05-23

申请号：US16954235

申请日：2018-12-31

申请人： LUXEMBOURG INSTITUTE OF SCIENCE AND TECHNOLOGY (LIST)

发明人： Arno Gutleb ,  Tommaso Serchi ,  Mathieu Gerard

IPC分类号： C12M1/42 ,  C12M1/00 ,  C12M1/34

CPC分类号： C12M35/04 ,  C12M29/14 ,  C12M41/14 ,  C12M41/40

摘要： A system for incubating one or more cells and/or organotypic cultures for biological investigation, in particular for toxicology assessment, comprising a bio-incubator and a pressure system fluidly connected with the bio-incubator. The pressure system is a cyclic gas pressure system configured for cyclically varying the gas pressure in the bio-incubator between a negative pressure and a positive pressure compared to the atmospheric pressure, so as to reproduce the pressure conditions in lungs of a living mammal. The system is remarkable in that the cyclic gas pressure system comprises a feedthrough with a pipe configured to deviate the air influx from the one or more cells and/or organotypic cultures.

公开(公告)号：US20180085726A1

公开(公告)日：2018-03-29

申请号：US15562447

申请日：2016-03-08

申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

发明人： Shinji SUGIURA ,  Taku SATOH ,  Toshiyuki KANAMORI ,  Kazumi SHIN

IPC分类号： B01J19/00 ,  C12M1/00 ,  C12M1/04 ,  C12M1/12 ,  B01F13/00 ,  C12M1/36 ,  B01L3/00

CPC分类号： B01J19/0046 ,  B01F13/0059 ,  B01L3/502 ,  B01L3/502738 ,  B01L2200/0621 ,  B01L2300/0829 ,  B01L2300/0874 ,  B01L2300/088 ,  B01L2400/0487 ,  B01L2400/0688 ,  C12M1/005 ,  C12M1/04 ,  C12M1/12 ,  C12M1/36 ,  C12M23/04 ,  C12M23/16 ,  C12M29/10 ,  C12M29/14

摘要： A cell culture apparatus, includes a connected culture container including n number of units disposed in parallel along a second direction that is a different direction from a first direction where each of the n number of the units is constituted of m number of culture chambers and one or more communication-channels, the m number of the culture chambers each having a cell-holding portion that holds seeded cells, the m number of the culture chambers storing liquid culture media, the m number of the culture chambers being disposed in parallel along the first direction, the communication-channels communicating the m number of the culture chambers with each other; and a plurality of pneumatic pipes communicating same-row-disposed culture chambers that are disposed on a same row along the second direction with each other in the connected culture container.

公开(公告)号：US20170198249A1

公开(公告)日：2017-07-13

申请号：US15323977

申请日：2014-07-23

申请人： Hitachi, Ltd.

发明人： Masaharu KIYAMA ,  Guangbin ZHOU ,  Takayuki NOZAKI ,  Ryota NAKAJIMA ,  Daisuke SUZUKI ,  Yumiko IGARASHI ,  Shizu TAKEDA ,  Taku NAKAMURA ,  Masakazu SUGAYA ,  Koichi TERADA

IPC分类号： C12M1/34 ,  C12M1/00

CPC分类号： C12M41/40 ,  C12M23/14 ,  C12M23/40 ,  C12M29/00 ,  C12M29/14 ,  C12M29/26 ,  C12M41/48

摘要： There is the problem that a liquid culture medium fed by a pump has a high frequency of contact with a gas during movement in a tube, and the pH value of the liquid culture medium before and after feeding changes easily. In a liquid feeder and a cell culture apparatus provided therewith, a feed pipe 7 is connected with a gas bag 14, the liquid to be fed into the feed pipe 7 is fed so as to be sandwiched by a gas for suppressing the qualitative changes of the liquid. Furthermore, the gas bag is connected with a container 8 for holding a liquid, and holds the gas for suppressing qualitative changes of the liquid in a gas phase of the container.

公开(公告)号：US20170159003A1

公开(公告)日：2017-06-08

申请号：US15327208

申请日：2015-07-08

申请人： HITACHI HIGH-TECHNOLOGIES CORPORATION

发明人： Akihiro SHIMASE ,  Kazumichi IMAI ,  Sadamitsu ASO ,  Eiichiro TAKADA ,  Masako KAWARAI ,  Toshinari SAKURAI

IPC分类号： C12M1/34 ,  C12M1/02 ,  C12M1/36 ,  C12M1/00

CPC分类号： C12M41/40 ,  C12M23/58 ,  C12M27/00 ,  C12M29/14 ,  C12M29/18 ,  C12M31/10 ,  C12M41/06 ,  C12M41/36 ,  C12M41/48 ,  C12Q1/06

摘要： A device which automatically performs a step in which expanded and cultured cells are diluted to a desired cell concentration and re-inoculated using a cell-concentration adjustment device having an inlet for taking in a cell suspension; an outlet for discharging a diluted cell suspension; and a flow path which is provided between the inlet and the outlet and is capable of holding a cell suspension, the flow path being provided with: a liquid delivery pump for causing a cell suspension inside to flow; a cell-concentration measurement instrument for collecting data related to a cell concentration per unit amount of the cell suspension; and a dilution-liquid container for holding a dilution liquid which is supplied to the flow path to dilute the cell suspension. The device further includes a control unit for controlling at least the liquid delivery pump on the basis of the data obtained by the cell-concentration measurement instrument, wherein the control unit determines, on the basis of the data obtained by the cell-concentration measurement instrument, an amount of the dilution liquid required to bring the cell concentration to the desired concentration, and drives the liquid delivery pump so as to take in the required amount of the dilution liquid into the flow path and mix the cell suspension and the dilution liquid.