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公开(公告)号：US4173516A
公开(公告)日：1979-11-06
申请号：US840165
申请日：1977-10-06
摘要： A method of cultivating cells of animal and human tissues includes the steps of introducing the cells into a nutrient solution in which they tend to settle, and maintaining the cells in the solution in a suspended state so as to prevent their settling by introducing an oxygen-containing gas into the solution. An apparatus for carrying out the method includes a container for a nutrient solution, an arrangement for introducing the oxygen-containing gas into the container, and a guide arrangement in the container and operative for guiding the nutrient solution so that the latter performs a circulatory movement in the container when the oxygen-containing gas is introduced into the latter. The guide arrangement may be formed as a tubular member, as a substantially flat partition wall, or as an inner wall of a circumferentially complete tubular container.
摘要翻译： 培养动物和人体组织的细胞的方法包括以下步骤：将细胞导入其倾向于沉降的营养液中，并将细胞保持在悬浮状态，以便通过引入氧 - 含有气体进入溶液。 用于实施该方法的装置包括用于营养液的容器，用于将含氧气体引入容器中的装置，以及容器中的引导装置，并用于引导营养液，使得其进行循环运动 在含氧气体被引入后者的容器中。 引导装置可以形成为管状构件，作为基本平坦的分隔壁，或形成为周向完整的管状容器的内壁。
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2.发明授权
公开(公告)号：US4311798A
公开(公告)日：1982-01-19
申请号：US015282
申请日：1979-02-26
CPC分类号： C12M29/14 , C12M23/34 , Y10S435/818
摘要： A method of cultivating cells of animal and human tissues includes the steps of introducing the cells into a nutrient solution in which they tend to settle, and maintaining the cells in the solution in a suspended state so as to prevent their settling by introducing an oxygen-containing gas to the solution. An apparatus for carrying out the method includes a container for a nutrient solution, an arrangement for introducing the oxygen-containing gas into the container, and a guide arrangement in the container and operative for guiding the nutrient solution so that the latter performs a circulatory movement in the container when the oxygen-containing gas is introduced into the latter. The guide arrangement may be formed as a tubular member, as a substantially flat partition wall, or as an inner wall of a circumferentially complete tubular container.
摘要翻译： 培养动物和人体组织的细胞的方法包括以下步骤：将细胞导入其倾向于沉降的营养液中，并将细胞保持在悬浮状态，以便通过引入氧 - 含有气体到溶液中。 用于实施该方法的装置包括用于营养液的容器，用于将含氧气体引入容器中的装置，以及容器中的引导装置，并用于引导营养液，使得其进行循环运动 在含氧气体被引入后者的容器中。 引导装置可以形成为管状构件，作为基本平坦的分隔壁，或形成为周向完整的管状容器的内壁。
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公开(公告)号：US4259449A
公开(公告)日：1981-03-31
申请号：US936600
申请日：1978-08-23
CPC分类号： C12M29/14 , C12M23/34 , Y10S435/818
摘要： A method of cultivating cells of animal and human tissues includes the steps of introducing the cells into a nutrient solution in which they tend to settle, and maintaining the cells in the solution in a suspended state so as to prevent their settling by introducing an oxygen-containing gas into the solution. An apparatus for carrying out the method includes a container for a nutrient solution, an arrangement for introducing the oxygen-containing gas into the container, and a guide arrangement in the container and operative for guiding the nutrient solution so that the latter performs a circulatory movement in the container when the oxygen-containing gas is introduced into the latter. The guide arrangement may be formed as a tubular member, as a substantially flat partition wall, or as an inner wall of a circumferentially complete tubular container.
摘要翻译： 培养动物和人体组织的细胞的方法包括以下步骤：将细胞导入其倾向于沉降的营养液中，并将细胞保持在悬浮状态，以便通过引入氧 - 含有气体进入溶液。 用于实施该方法的装置包括用于营养液的容器，用于将含氧气体引入容器中的装置，以及容器中的引导装置，并用于引导营养液，使得其进行循环运动 在含氧气体被引入后者的容器中。 引导装置可以形成为管状构件，作为基本平坦的分隔壁，或形成为周向完整的管状容器的内壁。
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4.发明授权Liposomal composition comprising an active ingredient for relaxing smooth muscle, the production of this composition and the therapeutic use thereof 有权包含用于松弛平滑肌的活性成分的脂质体组合物，该组合物的制备及其治疗用途公开(公告)号：US08524274B2
公开(公告)日：2013-09-03
申请号：US11577488
申请日：2005-10-14
CPC分类号： A61K9/127 , A61K9/0034 , A61K9/06 , A61K9/1277 , A61K9/1278
摘要： The invention relates to a pharmaceutical composition made from an active ingredient included in liposomes for topical application, whereby the liposomes have an aqueous medium in the interior thereof and contain at least one active ingredient therein which exerts a direct or indirect relaxing effect on smooth musculature and is preferably selected from the group of prostaglandins, adenylate cyclases, cAMP, AMP, ATP, NO-synthetases, nitrogen monoxide (NO), NO compounds, nitrates, guanylate cyclases, cGMP, GMP, GTP and phosphodiesterases, in particular, Sildenafil. The invention further relates to a method for production of said composition, optionally, in sterile form and the use of the liposomes supporting the active ingredient in various galenic forms for external application in the genital region, for prophylaxis and/or therapy of sexual disorders in men or women and/or for increase of sexual sensitivity.
摘要翻译： 本发明涉及由用于局部应用的脂质体中包含的活性成分制成的药物组合物，其中所述脂质体在其内部具有水性介质并且含有其中的至少一种活性成分，其对平滑肌肉组织施加直接或间接的缓解作用， 优选选自前列腺素，腺苷酸环化酶，cAMP，AMP，ATP，NO-合成酶，一氧化氮（NO），NO化合物，硝酸盐，鸟苷酸环化酶，cGMP，GMP，GTP和磷酸二酯酶，特别是西地那非。 本发明进一步涉及用于生产所述组合物的方法，任选地以无菌形式，以及使用支持活性成分的各种盖仑样形式的外源性生物区域用于预防和/或治疗性障碍的用途 男性或女性和/或增加性敏感性。
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5.发明授权Peptides that induce antibodies which neutralize genetically divergent HIV-1 isolates 失效诱导中和遗传上不同的HIV-1分离株的抗体的肽
公开(公告)号：US5693752A
公开(公告)日：1997-12-02
申请号：US361479
申请日：1994-12-22
申请人： Hermann Katinger , Florian Ruker , Gottfried Himmler , Thomas Muster , Alexandra Trkola , Martin Purtscher , Georg Maiwald , Franz Steindl
发明人： Hermann Katinger , Florian Ruker , Gottfried Himmler , Thomas Muster , Alexandra Trkola , Martin Purtscher , Georg Maiwald , Franz Steindl
IPC分类号： A61K38/00 , A61K39/00 , A61K39/21 , A61P31/12 , A61P31/18 , C07K7/06 , C07K14/16 , C07K16/00 , C07K16/10 , C07K16/42 , C07K19/00 , C12N15/09 , C12N15/49 , C12P21/02 , C12P21/08 , C12R1/19 , A61K38/04 , A61K39/38 , C07K5/00
CPC分类号： C07K14/005 , C07K16/1063 , C07K16/4225 , A61K39/00 , C07K2319/00 , C12N2740/16122
摘要： This invention refers to peptides binding to antibodies that show neutralizing activity against different strains and clinical isolates of HIV-1 and that inhibit the fusion of cells caused by HIV-1. These peptides are applied with an adjuvant, as recombinant fusion proteins, chemically coupled to carrier molecules, as recombinant chimeric viruses or as recombinant antibodies.
摘要翻译： 本发明涉及结合抗体的肽，其显示针对HIV-1的不同菌株和临床分离物的中和活性，并且抑制由HIV-1引起的细胞融合。 这些肽用作为重组融合蛋白的佐剂施用，作为重组嵌合病毒或作为重组抗体与载体分子化学偶联。
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6.发明申请LIPOSOMAL COMPOSITION COMPRISING AN ACTIVE INGREDIENT FOR RELAXING SMOOTH MUSCLE, THE PRODUCTION OF THIS COMPOSITION AND THE THERAPEUTIC USE THEREOF 有权包含用于放松平滑肌肉的活性成分的脂质组合物，该组合物的生产及其治疗用途公开(公告)号：US20090324698A1
公开(公告)日：2009-12-31
申请号：US11577488
申请日：2005-10-14
IPC分类号： A61K9/127 , A61P15/00 , A61K31/519 , A61K31/4985
CPC分类号： A61K9/127 , A61K9/0034 , A61K9/06 , A61K9/1277 , A61K9/1278
摘要： The invention relates to a pharmaceutical composition made from an active ingredient included in liposomes for topical application, whereby the liposomes have an aqueous medium in the interior thereof and contain at least one active ingredient therein which exerts a direct or indirect relaxing effect on smooth musculature and is preferably selected from the group of prostaglandins, adenylate cyclases, cAMP, AMP, ATP, NO-synthetases, nitrogen monoxide (NO), NO compounds, nitrates, guanylate cyclases, cGMP, GMP, GTP and phosphodiesterases, in particular, Sildenafil. The invention further relates to a method for production of said composition, optionally, in sterile form and the use of the liposomes supporting the active ingredient in various galenic forms for external application in the genital region, for prophylaxis and/or therapy of sexual disorders in men or women and/or for increase of sexual sensitivity.
摘要翻译： 本发明涉及由用于局部应用的脂质体中包含的活性成分制成的药物组合物，其中所述脂质体在其内部具有水性介质并且含有其中的至少一种活性成分，其对平滑肌肉组织施加直接或间接的缓解作用， 优选选自前列腺素，腺苷酸环化酶，cAMP，AMP，ATP，NO-合成酶，一氧化氮（NO），NO化合物，硝酸盐，鸟苷酸环化酶，cGMP，GMP，GTP和磷酸二酯酶，特别是西地那非。 本发明进一步涉及用于生产所述组合物的方法，任选地以无菌形式，以及使用支持活性成分的各种盖仑样形式的外源性生物区域用于预防和/或治疗性障碍的用途 男性或女性和/或增加性敏感性。
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公开(公告)号：US07595049B2
公开(公告)日：2009-09-29
申请号：US10485525
申请日：2002-09-09
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/505 , C07K16/1063 , C07K2317/21 , C07K2317/34 , C12N2740/16122
摘要： The present invention relates to neutralizing anti-HIV-1 antibodies, particularly to mAb 4E10-IgG1, which has an HIV-1 neutralizing potency comparable to the one of mAb 2F5 and 2G12. 4E10-IgG1 binds to a novel conserved epitope (NWFDIT) C-terminal of the ELDKWA epitope recognized by 2F5.1 appears that both epitopes are cryptic epitopes within a region that may be accessible in a virus-cell fusion intermediate state only. 4E10-IgG1 potently neutralizes tissue culture adapted strains but also primary isolates of different clades, including A, B, C, D, and E, inclusing viruses that were found to be resistant to 2F5. None of the tested isolates was resistant to both anti-gp41-antibodies. The invention therefore also relates to peptides containing the 4E10 epitope and to compositions made thereof, as well as to anti-idiotypic antibodies that are reactive with the paratope of 4E10-IgG1, to compositions containing an antiidiotypic antibody optionally in combination with a peptide containing the 4E10 epitope, and to anti-HIV-1 compositions comprising 4E10-IgG1, optionally in combination with another neutralizing antibody such as 2F5 and/or 2G12.
摘要翻译： 本发明涉及中和抗HIV-1抗体，特别是抗mAb 4E10-IgG1，其具有与mAb 2F5和2G12中的一种相当的HIV-1中和效力。 4E10-IgG1与2F5.1识别的ELDKWA表位的新保守表位（NWFDIT）C-末端结合似乎是两个表位是仅在病毒 - 细胞融合中间状态可以接近的区域内的隐性表位。 4E10-IgG1有力地中和组织培养适应菌株，也可以中和不同进化枝的初级分离物，包括A，B，C，D和E，包括被发现对2F5有抗性的病毒。 没有一个测试的分离株对抗gp41-抗体均有抗性。 因此，本发明还涉及含有4E10表位的肽及其制备的组合物，以及与4E10-IgG1的相互作用反应的抗独特型抗体，所述组合物含有抗独立型抗体，所述组合物任选地与含有 4E10表位，以及包含4E10-IgG1的抗HIV-1组合物，任选地与另一种中和抗体如2F5和/或2G12组合。
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公开(公告)号：US07351549B2
公开(公告)日：2008-04-01
申请号：US10181808
申请日：2001-01-24
申请人： Diethard Mattanovich , Hermann Katinger , Hubertus Hohenblum , Stefan Naschberger , Robert Weik
发明人： Diethard Mattanovich , Hermann Katinger , Hubertus Hohenblum , Stefan Naschberger , Robert Weik
CPC分类号： C12N9/6427
摘要： The present invention relates to a method for the manufacture and purification of recombinant trypsinogen and trypsin in E. coli and yeast, using high yield expression vectors with and without secretion leader sequences. The invention further relates to an improved method and apparatus for carrying out protein refolding specifically useful for processing trypsinogen that has accumulated intracellularly in the form of inclusion bodies.
摘要翻译： 本发明涉及使用具有和不具有分泌前导序列的高产量表达载体在大肠杆菌和酵母中制造和纯化重组胰蛋白酶原和胰蛋白酶的方法。 本发明还涉及一种用于进行蛋白质重折叠的改进方法和装置，其特别可用于加工以包涵体形式累积在细胞内的胰蛋白酶原。
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公开(公告)号：US07202352B2
公开(公告)日：2007-04-10
申请号：US10181059
申请日：2001-01-22
CPC分类号： C07K14/4747
摘要： The present invention relates to cell-regulatory proteins and to nucleotide sequences encoding said proteins wherein the proteins have been found to be potential tumor and/or senescense markers useful for medical diagnostics and additionally having therapeutical potential as well as antiapoptotic properties useful for improving cell viability in cell culture technology. The invention further relates to antibodies directed against the cell-regulatory proteins and to their use in medical diagnostics and therapy. The invention further relates to methods for the manufacture and application of the cell-regulatory proteins and of the corresponding nucleotide sequences.
摘要翻译： 本发明涉及细胞调节蛋白和编码所述蛋白质的核苷酸序列，其中所述蛋白质已经被发现是潜在的肿瘤和/或用于医学诊断的并且具有治疗潜力的抗衰老标记物，以及可用于改善细胞活力的抗细胞凋亡性质 在细胞培养技术中。 本发明还涉及针对细胞调节蛋白的抗体及其在医学诊断和治疗中的应用。 本发明还涉及制造方法
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公开(公告)号：US20050080240A1
公开(公告)日：2005-04-14
申请号：US10501726
申请日：2003-01-17
CPC分类号： C07K16/4225 , A61K39/00 , A61K2039/505
摘要： The invention relates to Ab2-type anti-idiotypic antibodies and fragments thereof which mimic HVI-1 epitopes that are otherwise cryptic to the immune system and which antibodies or fragments thereof are directed against potently neutralizing anti-HIV-1 antibodies. The invention further relates to a hybridoma cell line 3H6 expressing the anti-idiotypic antibody and to pharmaceutical compositions containing the antibody or fragment thereof. The invention also relates to HIV-1 neutralizing Ab3-type antibodies elicited upon administration of the Ab2-type anti-idiotypic antibody or fragment thereof and to pharmaceutical compositions containing them. The invention also relates to the use of the present antibodies or fragments thereof as screening tools or as diagnostic or therapeutic agents.
摘要翻译： 本发明涉及Ab2型抗独特型抗体及其片段，其模拟HVI-1表位，否则其对于免疫系统是隐秘的，哪些抗体或其片段针对有效中和抗HIV-1抗体。 本发明还涉及表达抗独特型抗体的杂交瘤细胞系3H6和含有抗体或其片段的药物组合物。 本发明还涉及在施用Ab2型抗独特型抗体或其片段时引发的HIV-1中和Ab3型抗体以及含有它们的药物组合物。 本发明还涉及本发明的抗体或其片段作为筛选工具或诊断或治疗剂的用途。
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