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公开(公告)号:US09090912B1
公开(公告)日:2015-07-28
申请号:US13254770
申请日:2010-03-04
CPC分类号: C12N15/88 , A61K9/1271 , A61K9/1272 , A61K9/1278 , A61K31/7088 , A61K31/7105 , A61K47/36 , A61K47/40 , C12N15/111 , C12N2310/14 , C12N2310/3515 , C12N2320/32
摘要: This invention relates to a nucleic acid complex with low toxicity and high safety that can maintain a nucleic acid molecule capable of RNA interference or translation inhibition, such as siRNA or the like, in a cell; and a nucleic acid delivery composition that can efficiently deliver the nucleic acid complex into a cell. A nucleic acid complex with low toxicity and high safety that can maintain a nucleic acid in a cell can be obtained by forming a complex using a nucleic acid molecule capable of RNA interference or translation inhibition and a cycloamirose. Further, by using a carrier comprising (A) a diacylphosphatidylcholine, (B) cholesterol and/or a derivative thereof, and (C) an aliphatic primary amine as a nucleic acid delivery carrier to introduce the nucleic acid complex into a cell, the safety, efficiency of intracellular delivery, and persistence of the nucleic acid in the cell can be further improved.
摘要翻译: 本发明涉及一种具有低毒性和高安全性的核酸复合物,其能够在细胞中维持能够进行RNA干扰或翻译抑制的核酸分子,例如siRNA等; 以及可以有效地将核酸复合物递送到细胞中的核酸递送组合物。 可以通过使用能够进行RNA干扰或翻译抑制的核酸分子和循环波形来形成复合物来获得能够维持细胞中核酸的低毒性和高安全性的核酸复合物。 此外,通过使用包含(A)二酰基磷脂酰胆碱,(B)胆固醇和/或其衍生物的载体,和(C)作为核酸递送载体的脂族伯胺将核酸复合物引入细胞, 可以进一步提高细胞内递送的效率和核酸在细胞中的持续性。
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公开(公告)号:US08338584B2
公开(公告)日:2012-12-25
申请号:US12741457
申请日:2008-11-06
CPC分类号: A61K47/40 , A61K9/1272 , A61K47/543 , A61K47/6951 , A61K48/005 , B82Y5/00 , C12N15/88
摘要: The present invention provides a nucleic acid complex with low toxicity and high safety that can persistently maintain a nucleic acid, such as siRNA or the like, in a cell; and a nucleic acid delivery composition that can efficiently deliver the nucleic acid complex into a cell. A nucleic acid complex with low toxicity and high safety that can persistently maintain a nucleic acid in a cell can be obtained by forming a complex using a nucleic acid to be introduced into a cell, and a highly branched cyclic dextrin. Moreover, when a carrier comprising (A) a diacylphosphatidylcholine, (B) cholesterol and/or a derivative thereof, and (C) an aliphatic primary amine is used as a nucleic acid delivery carrier to introduce the nucleic acid complex into a cell, the safety, the efficiency of intracellular delivery, and the persistence of the nucleic acid in the cell can be further improved.
摘要翻译: 本发明提供了一种具有低毒性和高安全性的核酸复合物,其可以在细胞中持续保持核酸如siRNA等; 以及可以有效地将核酸复合物递送到细胞中的核酸递送组合物。 可以通过使用待引入细胞的核酸和高度支化的环糊精形成复合物来获得能够持续维持细胞中核酸的低毒性和高安全性的核酸复合物。 此外,当使用包含(A)二酰基磷脂酰胆碱,(B)胆固醇和/或其衍生物和(C)脂肪族伯胺的载体作为核酸递送载体以将核酸复合物引入细胞时, 可以进一步提高安全性,细胞内递送的效率和核酸在细胞中的持续性。
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公开(公告)号:US20100305190A1
公开(公告)日:2010-12-02
申请号:US12741457
申请日:2008-11-06
IPC分类号: C07H21/02 , C07H21/00 , A61K31/7105 , C12N5/00
CPC分类号: A61K47/40 , A61K9/1272 , A61K47/543 , A61K47/6951 , A61K48/005 , B82Y5/00 , C12N15/88
摘要: The present invention provides a nucleic acid complex with low toxicity and high safety that can persistently maintain a nucleic acid, such as siRNA or the like, in a cell; and a nucleic acid delivery composition that can efficiently deliver the nucleic acid complex into a cell. A nucleic acid complex with low toxicity and high safety that can persistently maintain a nucleic acid in a cell can be obtained by forming a complex using a nucleic acid to be introduced into a cell, and a highly branched cyclic dextrin. Moreover, when a carrier comprising (A) a diacylphosphatidylcholine, (B) cholesterol and/or a derivative thereof, and (C) an aliphatic primary amine is used as a nucleic acid delivery carrier to introduce the nucleic acid complex into a cell, the safety, the efficiency of intracellular delivery, and the persistence of the nucleic acid in the cell can be further improved.
摘要翻译: 本发明提供了一种具有低毒性和高安全性的核酸复合物,其可以在细胞中持续保持核酸如siRNA等; 以及可以有效地将核酸复合物递送到细胞中的核酸递送组合物。 可以通过使用待引入细胞的核酸和高度支化的环糊精形成复合物来获得能够持续维持细胞中核酸的低毒性和高安全性的核酸复合物。 此外,当使用包含(A)二酰基磷脂酰胆碱,(B)胆固醇和/或其衍生物和(C)脂肪族伯胺的载体作为核酸递送载体以将核酸复合物引入细胞时, 可以进一步提高安全性,细胞内递送的效率和核酸在细胞中的持续性。
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4.
公开(公告)号:US20110189311A1
公开(公告)日:2011-08-04
申请号:US12870979
申请日:2010-08-30
CPC分类号: A61K31/10 , A61K9/0053 , A61K9/1623 , A61K31/192
摘要: Provided is a highly absorbable drug composition including one or more water-soluble compound (A) selected from an enzyme-treated hesperidin, a stevia extract, or an enzyme-treated stevia and a poorly water-soluble drug (B) selected from flurbiprofen or probucol.
摘要翻译: 提供了一种高吸收性药物组合物,其包含一种或多种选自酶治疗的橙皮苷,甜叶菊提取物或经酶处理的甜菊中的水溶性化合物(A)和选自氟比洛芬或弱水溶性药物 普罗布洛
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