公开(公告)号：US07715012B2

公开(公告)日：2010-05-11

申请号：US12394298

申请日：2009-02-27

申请人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

发明人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

IPC分类号： G01N21/55

CPC分类号： G01N21/05 ,  G01N21/553 ,  G01N2021/0346 ,  G01N2021/058

摘要： A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio) chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

摘要翻译： 表面等离子体共振（SPR）测定系统的传感器单元包括透明电介质。 薄膜具有第一表面和感测表面。 第一表面与电介质连接以构成界面。 感测表面回到第一个表面，用于检测（生物）化学反应。 流动池块具有用于将样品流动到感测表面的流动通道。 在界面处检查照明光的衰减全反射，分析配体和分析物之间的相互作用作为样品。 流动通道包括与感测表面相对设置的第一内表面，以沿着该方式延伸以使样品在两者之间流动。 第一内表面具有相对于感测表面限定的高度，并且在200-500微米的范围内。

公开(公告)号：US20090195784A1

公开(公告)日：2009-08-06

申请号：US12394298

申请日：2009-02-27

申请人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

发明人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

IPC分类号： G01N21/55 ,  G01N21/05

CPC分类号： G01N21/05 ,  G01N21/553 ,  G01N2021/0346 ,  G01N2021/058

摘要： A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio) chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

摘要翻译： 表面等离子体共振（SPR）测定系统的传感器单元包括透明电介质。 薄膜具有第一表面和感测表面。 第一表面与电介质连接以构成界面。 感测表面回到第一个表面，用于检测（生物）化学反应。 流动池块具有用于将样品流动到感测表面的流动通道。 在界面处检查照明光的衰减全反射，分析配体和分析物之间的相互作用作为样品。 流动通道包括与感测表面相对设置的第一内表面，以沿着该方式延伸以使样品在两者之间流动。 第一内表面具有相对于感测表面限定的高度，并且在200-500微米的范围内。

公开(公告)号：US06781143B2

公开(公告)日：2004-08-24

申请号：US10116701

申请日：2002-04-05

申请人： Hitoshi Shimizu ,  Nobuhiko Ogura

发明人： Hitoshi Shimizu ,  Nobuhiko Ogura

IPC分类号： G03B4200

CPC分类号： G01T1/2014 ,  G01T1/2012 ,  G01T1/2985

摘要： A biochemical analysis data producing method includes the steps of exposing stimulable phosphor layer regions formed in a stimulable phosphor sheet and spaced from each other using standard light, irradiating the stimulable phosphor layer regions with a stimulating ray, detecting stimulated emission to produce correction data for the individual stimulable phosphor layer regions, superposing the stimulable phosphor sheet on a biochemical analysis unit including spot-like regions formed in the same pattern as that of the stimulable phosphor layer regions and selectively containing a radioactive labeling substance, exposing the stimulable phosphor layer regions to the radioactive labeling substance, scanning the stimulable phosphor layer regions with a stimulating ray, detecting stimulated emission to produce biochemical analysis data, and correcting the biochemical analysis data using the correction data. According to this biochemical analysis data producing method, it is possible to produce biochemical analysis data having excellent quantitative characteristics.

摘要翻译： 生物化学分析数据生成方法包括以下步骤：使用标准光曝光形成在可刺激的荧光体片材中的可激发的荧光体层区域，并用刺激的光线照射可刺激的荧光体层区域，检测受激发射以产生校正数据 单独的可刺激荧光体层区域，将可刺激的荧光体片材叠加在生物化学分析单元上，该生物化学分析单元包括以与可刺激的荧光体层区域相同的图案形成的斑点状区域，并选择性地含有放射性标记物质，将可刺激的荧光体层区域暴露于 放射性标记物质，用刺激射线扫描可刺激的荧光体层区域，检测受激发射以产生生化分析数据，以及使用校正数据校正生化分析数据。 根据该生化分析数据生成方法，能够生成具有优异的定量特性的生化分析数据。

公开(公告)号：US07348587B2

公开(公告)日：2008-03-25

申请号：US10175425

申请日：2002-06-20

申请人： Hitoshi Shimizu ,  Nobuhiko Ogura

发明人： Hitoshi Shimizu ,  Nobuhiko Ogura

IPC分类号： G03B42/08

CPC分类号： G01N21/76 ,  G01N21/6452 ,  G01N2021/6484

摘要： A method for producing biochemical analysis data includes the steps of collecting light selectively released from a plurality of light releasable regions two-dimensionally formed to be spaced apart from each other in a sample placed on a sample stage by a plurality of light guide member each of which is disposed to face one of the plurality of light releasable regions, leading the thus collected light to a light detector and photoelectrically detecting the light by the light detector. According to this method, it is possible to produce biochemical analysis data having high quantitative characteristics by photoelectrically detecting light emitted from a plurality of light releasable regions even in the case where the plurality of light releasable regions labeled with a labeling substance are formed in a sample at a high density.

摘要翻译： 一种生化分析数据的制造方法，其特征在于，在多个导光部件中，通过多个导光部件收集从放置在样品台上的样品彼此间隔开的二维形成的多个可光释放区域中选择性地剥离的光 其被设置为面对多个可光释放区域中的一个，将由此收集的光导向光检测器并通过光检测器光电检测光。 根据该方法，即使在标记有标记物质的多个可光剥离区域形成在样品中的情况下，也可以通过光电检测从多个可光释放区域发出的光来生成具有高定量特性的生化分析数据 以高密度。

公开(公告)号：USD561055S1

公开(公告)日：2008-02-05

申请号：US29266477

申请日：2006-09-22

申请人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Yasunobu Sakaguchi ,  Masayasu Konishi

设计人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Yasunobu Sakaguchi ,  Masayasu Konishi

公开(公告)号：US07170078B2

公开(公告)日：2007-01-30

申请号：US10141555

申请日：2002-05-09

申请人： Hitoshi Shimizu ,  Keiko Neriishi ,  Nobuhiko Ogura

发明人： Hitoshi Shimizu ,  Keiko Neriishi ,  Nobuhiko Ogura

IPC分类号： G03B42/08

CPC分类号： G01N21/6452 ,  G01N21/253 ,  G01N33/54373

摘要： A biochemical analysis data producing method includes the steps of selectively storing one of radiation energy and light energy in a plurality of stimulable phosphor layer regions two-dimensionally formed in a support of a stimulable phosphor sheet and spaced apart from each other, moving the stimulable phosphor sheet and a stimulating ray relative to each other in a main scanning direction and a sub-scanning direction, sequentially irradiating the plurality of stimulable phosphor layer regions so that energy of the stimulating ray projected onto the plurality of stimulable phosphor layer regions per unit area is higher than that projected on regions other than the plurality of stimulable phosphor layer regions, thereby exciting stimulable phosphor contained in the plurality of stimulable phosphor layer regions, and photoelectrically detecting stimulated emission released from the stimulable phosphor layer to produce biochemical analysis data. According to this method, it is possible to effectively prevent the stimulating ray from entering a neighboring stimulable phosphor layer region to be next stimulated as the stimulating ray is scanned and thus prevent stimulable phosphor contained in the neighboring stimulable phosphor layer region from being excited to release radiation energy or light energy stored therein, and, therefore, biochemical analysis data having an excellent quantitative characteristic can be produced in a desired manner.

摘要翻译： 生物化学分析数据生成方法包括以下步骤：将辐射能量和光能中的一种选择性地存储在可激发的荧光体片的支撑体中二维地形成并且彼此间隔开的多个可刺激的荧光体层区域中，将可刺激的荧光体 并且在主扫描方向和副扫描方向上相对于彼此产生刺激光线，顺序地照射多个可刺激的荧光体层区域，使得投影到每单位面积的多个可激发荧光体层区域上的刺激光线的能量为 高于在多个可刺激的荧光体层区域以外的区域上投影的激光荧光体层，从而激发包含在多个可刺激的荧光体层区域中的可激发的荧光体，以及光电检测从可刺激的荧光体层释放的受激发射以产生生化分析数据。 根据该方法，可以有效地防止刺激光线随着刺激射线被扫描而进入下一个被刺激的刺激性射线区域，从而防止相邻可刺激荧光体层区域中包含的可刺激的荧光体被激发释放 辐射能量或光能存储在其中，因此可以以期望的方式产生具有优异定量特性的生化分析数据。

公开(公告)号：USD561623S1

公开(公告)日：2008-02-12

申请号：US29266470

申请日：2006-09-22

申请人： Nobuhiko Ogura ,  Katsuaki Muraishi ,  Hitoshi Shimizu ,  Masashi Hakamata ,  Yasunobu Sakaguchi ,  Hideyuki Morimoto ,  Hiroyuki Ota ,  Masayasu Konishi ,  Yoshiyuki Kunuki

设计人： Nobuhiko Ogura ,  Katsuaki Muraishi ,  Hitoshi Shimizu ,  Masashi Hakamata ,  Yasunobu Sakaguchi ,  Hideyuki Morimoto ,  Hiroyuki Ota ,  Masayasu Konishi ,  Yoshiyuki Kunuki

公开(公告)号：USD561053S1

公开(公告)日：2008-02-05

申请号：US29266439

申请日：2006-09-21

申请人： Nobuhiko Ogura ,  Katsuaki Muraishi ,  Hitoshi Shimizu ,  Masashi Hakamata ,  Yasunobu Sakaguchi ,  Yoshiyuki Kunuki

设计人： Nobuhiko Ogura ,  Katsuaki Muraishi ,  Hitoshi Shimizu ,  Masashi Hakamata ,  Yasunobu Sakaguchi ,  Yoshiyuki Kunuki

公开(公告)号：US20060197954A1

公开(公告)日：2006-09-07

申请号：US11367302

申请日：2006-03-06

申请人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

发明人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

IPC分类号： G01N21/55

CPC分类号： G01N21/05 ,  G01N21/553 ,  G01N2021/0346 ,  G01N2021/058

摘要： A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio)chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

公开(公告)号：US07625748B2

公开(公告)日：2009-12-01

申请号：US11475064

申请日：2006-06-27

申请人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Tatsuo Fujikura ,  Koji Kuruma

发明人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Tatsuo Fujikura ,  Koji Kuruma

IPC分类号： C12M3/00 ,  C12M1/34

CPC分类号： G01N21/553 ,  G01N21/05

摘要： A flow cell device is incorporated in the sensor unit for surface plasmon resonance (SPR) assay. A box shaped flow cell body has a lower surface, and is disposed in contact of the lower surface with a sensing surface for detecting reaction of sample. Two flow channels are formed in the flow cell body, for flow of the sample. Each of the flow channels include a fluid passageway, formed in the lower surface, for flow of the sample in contact with the sensing surface. Two end cavities are formed in an upper surface of the flow cell body and open therein, to extend from ends of the fluid passageway. The flow channels are arranged adjacent to one another, disposed to extend in a direction of the flow. Fluid passageways of the flow channels are overlapped partially on one another in a flow cell longitudinal direction of the flow cell body.

摘要翻译： 在用于表面等离子体共振（SPR）测定的传感器单元中并入流通池装置。 盒状流通池主体具有下表面，并且设置成与下表面接触，用于检测样品的反应。 在流动池体中形成两个流动通道用于样品的流动。 每个流动通道包括形成在下表面中的流体通道，用于与感测表面接触的样品的流动。 两个端腔形成在流动池主体的上表面并在其中打开，以从流体通道的端部延伸。 流动通道彼此相邻设置，设置成沿流动方向延伸。 流动通道的流体通道在流动池主体的流动池纵向上部分地彼此重叠。