公开(公告)号：US07144153B2

公开(公告)日：2006-12-05

申请号：US10929570

申请日：2004-08-31

申请人： Shu Sato

发明人： Shu Sato

IPC分类号： G01K13/00 ,  G01N21/55

CPC分类号： G01N21/553

摘要： In a surface plasmon resonance sensor, a sensor unit includes a dielectric block, a thin film layer which is formed on the upper surface of the dielectric block, and a sample holding portion. An incubator is provided, the surface plasmon resonance sensor is spatially isolated from the surroundings by placing it in the measuring system, the temperature of the measuring system is measured and temperature change of the sensor unit after it is conveyed to the measuring system from the incubator is estimated on the basis of the temperature difference between the temperature of the incubator and the temperature of the measuring system, and the sensor unit is conveyed to the measuring system to perform the measurement within a time for which the temperature of the sensor unit does not unacceptably change.

摘要翻译： 在表面等离子体共振传感器中，传感器单元包括介质块，形成在介质块的上表面上的薄膜层和样品保持部分。 提供培养箱，表面等离子体共振传感器通过将其放置在测量系统中与周围空间隔离，测量系统的温度和传感器单元的温度从培养箱传送到测量系统后的温度变化 是基于培养箱的温度与测量系统的温度之间的温差来估计的，并且传感器单元被传送到测量系统以在传感器单元的温度不在的时间内进行测量 不可接受地改​​变。

公开(公告)号：US20060197954A1

公开(公告)日：2006-09-07

申请号：US11367302

申请日：2006-03-06

申请人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

发明人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

IPC分类号： G01N21/55

CPC分类号： G01N21/05 ,  G01N21/553 ,  G01N2021/0346 ,  G01N2021/058

摘要： A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio)chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

公开(公告)号：US20060066861A1

公开(公告)日：2006-03-30

申请号：US11239305

申请日：2005-09-30

申请人： Shu Sato ,  Toshihito Kimura ,  Hisashi Ohtsuka

发明人： Shu Sato ,  Toshihito Kimura ,  Hisashi Ohtsuka

IPC分类号： G01N21/55

CPC分类号： G01N21/553

摘要： A measurement path is filled with air prior to performing actual measurement. A p-polarized light beam is caused to enter an interface, and the intensity distribution of the light beam reflected at the interface is detected by a photodiode array to obtain a reference intensity distribution of the light beam itself. Thereafter, the measurement path is filled with a target for measurement, and the intensity distribution of a light beam reflected at the interface is measured. Each of the measured distribution values are divided by the reference intensity distribution, to cancel out influences due to fluctuations in the intensity distribution of the light beam. Thereby, the position of an attenuated total reflection angle is detected with high accuracy. Because a light beam constituted by p-polarized light waves is utilized, separating means for separating the light beam reflected at the interface into p-polarized and s-polarized light waves becomes unnecessary.

摘要翻译： 在执行实际测量之前，测量路径充满空气。 使p偏振光束进入界面，并且通过光电二极管阵列检测在界面处反射的光束的强度分布，以获得光束本身的参考光强分布。 此后，用测量目标填充测量路径，并且测量在界面处反射的光束的强度分布。 每个测量分布值除以参考强度分布，以消除由于光束的强度分布的波动引起的影响。 从而，以高精度检测衰减的全反射角度的位置。 由于利用由p偏振光波构成的光束，所以不需要用于将在界面处反射的光束分离为p偏振光和S偏振光波的分离装置。

公开(公告)号：US07715012B2

公开(公告)日：2010-05-11

申请号：US12394298

申请日：2009-02-27

申请人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

发明人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

IPC分类号： G01N21/55

CPC分类号： G01N21/05 ,  G01N21/553 ,  G01N2021/0346 ,  G01N2021/058

摘要： A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio) chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

摘要翻译： 表面等离子体共振（SPR）测定系统的传感器单元包括透明电介质。 薄膜具有第一表面和感测表面。 第一表面与电介质连接以构成界面。 感测表面回到第一个表面，用于检测（生物）化学反应。 流动池块具有用于将样品流动到感测表面的流动通道。 在界面处检查照明光的衰减全反射，分析配体和分析物之间的相互作用作为样品。 流动通道包括与感测表面相对设置的第一内表面，以沿着该方式延伸以使样品在两者之间流动。 第一内表面具有相对于感测表面限定的高度，并且在200-500微米的范围内。

公开(公告)号：US20090195784A1

公开(公告)日：2009-08-06

申请号：US12394298

申请日：2009-02-27

申请人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

发明人： Nobuhiko Ogura ,  Hitoshi Shimizu ,  Koji Kuruma ,  Hisashi Ohtsuka ,  Tatsuo Fujikura ,  Shu Sato

IPC分类号： G01N21/55 ,  G01N21/05

CPC分类号： G01N21/05 ,  G01N21/553 ,  G01N2021/0346 ,  G01N2021/058

摘要： A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio) chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

摘要翻译： 表面等离子体共振（SPR）测定系统的传感器单元包括透明电介质。 薄膜具有第一表面和感测表面。 第一表面与电介质连接以构成界面。 感测表面回到第一个表面，用于检测（生物）化学反应。 流动池块具有用于将样品流动到感测表面的流动通道。 在界面处检查照明光的衰减全反射，分析配体和分析物之间的相互作用作为样品。 流动通道包括与感测表面相对设置的第一内表面，以沿着该方式延伸以使样品在两者之间流动。 第一内表面具有相对于感测表面限定的高度，并且在200-500微米的范围内。

公开(公告)号：US06885454B2

公开(公告)日：2005-04-26

申请号：US10108258

申请日：2002-03-28

申请人： Masayuki Naya ,  Nobufumi Mori ,  Toshihito Kimura ,  Hitoshi Shimizu ,  Shu Sato

发明人： Masayuki Naya ,  Nobufumi Mori ,  Toshihito Kimura ,  Hitoshi Shimizu ,  Shu Sato

IPC分类号： G01N21/55 ,  G01N21/41 ,  G02B17/00 ,  G02B26/08 ,  H04J14/08

CPC分类号： G01N21/553

摘要： A measuring apparatus is disclosed which includes a measuring unit equipped with a dielectric block and a thin film layer; an incidence system for making a light beam enter the dielectric block so that a condition for total internal reflection is satisfied at an interface between the dielectric block and the thin film layer; and a photodetector for receiving the light beam totally reflected at the interface. The measuring unit is measured a plurality of times, and a change in the state of attenuated total reflection during the plurality of measurements is detected. The sensor further includes a tilt measurement section for measuring the longitudinal tilt of the interface which changes the incidence angles during the plurality of measurements, and a calculating section for obtaining a measured value in which errors due to the longitudinal tilt have been corrected.

摘要翻译： 公开了一种测量装置，其包括配备有介质块和薄膜层的测量单元; 用于使光束进入介质块的入射系统使得在介电块和薄膜层之间的界面处满足全内反射条件; 以及用于接收在界面处全反射的光束的光电检测器。 测量测量单元多次，并且检测在多个测量期间衰减的全反射状态的变化。 传感器还包括用于测量在多个测量期间改变入射角的界面的纵向倾斜的倾斜测量部分，以及用于获得校正了由于纵向倾斜引起的误差的测量值的计算部分。

公开(公告)号：US07075657B2

公开(公告)日：2006-07-11

申请号：US10648509

申请日：2003-08-27

申请人： Shu Sato

发明人： Shu Sato

IPC分类号： G01N21/55

CPC分类号： G01N21/553

摘要： A measuring apparatus comprising a measuring chip, an optical incidence system, a photodiode array, a differentiation part, and a computation part. The differentiation part differentiates an optical detection signal output from each light-receiving element, in a direction where light-receiving elements are juxtaposed, at intervals of outputs of two adjacent light-receiving elements. The computation part specifies a reference light-receiving element, then judges whether or not values of the optical detection signals of a first predetermined number of light-receiving elements increase monotonously in directions going to both sides, and computes a position of a dark line on the basis of a value obtained by differentiating the outputs of a second predetermined number of light-receiving elements sandwiching the reference light-receiving element when it is judged that the values of the optical detection signals increase monotonously, in the above-described direction.

摘要翻译： 一种测量装置，包括测量芯片，光学入射系统，光电二极管阵列，微分部分和计算部分。 微分部件以两个相邻的光接收元件的输出的间隔，使光接收元件在光接收元件并置的方向上输出的光检测信号区分开。 计算部分指定参考光接收元件，然后判断第一预定数量的光接收元件的光检测信号的值是否在朝向两侧的方向上单调增加，并且计算暗线的位置 在上述方向判断光检测信号的值单调增加时，通过对夹着参考光接收元件的第二预定数量的光接收元件的输出进行微分而获得的值的基础。

公开(公告)号：US06693269B2

公开(公告)日：2004-02-17

申请号：US09922929

申请日：2001-08-07

申请人： Hitoshi Shimizu ,  Shu Sato ,  Taizo Akimoto

发明人： Hitoshi Shimizu ,  Shu Sato ,  Taizo Akimoto

IPC分类号： H01L2700

CPC分类号： G01T1/208

摘要： A photomultiplier which detects feeble light emitted by the irradiation of excitation light on an image carrier carrying an image related to a living organism has an integration circuit is DC-coupled with a photomultiplier to convert a current signal output from the photomultiplier, to a voltage signal. The voltage signal converted by the integration circuit is logarithmically compressed with a logarithmic conversion circuit. An offset compensation circuit is provided for reducing an offset voltage that could occur because of charge injection in the integral action of the integration circuit.

摘要翻译： 检测由携带有机体的图像的图像载体上的激发光照射发出的微弱光的光电倍增管与光电倍增管DC耦合，将从光电倍增管输出的电流信号转换为电压信号 。 由积分电路转换的电压信号用对数转换电路进行对数压缩。 提供了一种偏移补偿电路，用于减少由于集成电路的积分作用中的电荷注入而可能发生的偏移电压。

公开(公告)号：US4939667A

公开(公告)日：1990-07-03

申请号：US138395

申请日：1987-12-28

申请人： Makoto Hara ,  Shu Sato

发明人： Makoto Hara ,  Shu Sato

IPC分类号： G01N33/50 ,  G01N27/447 ,  G01N33/58 ,  G01T1/00 ,  G01T1/29 ,  G06T1/00

CPC分类号： G01N27/44717 ,  Y10T436/143333

摘要： A signal processing method for analyzing an autoradiograph by subjecting digital image data containing an autoradiograph of a resolved pattern to digital signal processing to obtain locational information on radioactively labeled substances in the form of numerals and/or symbols is disclosed. The resolved pattern is formed by resolving radioactively labeled substances, such as, for example, nucleic acids, in a one-dimensional direction on a support medium. The total boundary area of the resolved patterns throughout the digital image data is determined by first dividing the digital image data into two or more blocks along a direction perpendicular to the resolving direction and then preparing a waveform composed of positions along the direction perpendicular to the resolving direction for each block by compiling the digital image date for each block. The waveform is used to establish the boundary of the area of the resolved pattern for each block, and the boundaries of the resolved pattern area of each block are interpolated to establish the total boundary area of the resolved patterns. This method is an easily conducted, accurate analytical method for obtaining locational information and, thus, the identity of a wide variety of radioactively labeled substances.

摘要翻译： 公开了一种信号处理方法，用于通过使包含解析图案的放射自显影图像的数字图像数据进行数字信号处理来分析放射自显影仪，以获得数字和/或符号形式的放射性标记物质的位置信息。 通过在载体介质上的一维方向上分解放射性标记的物质，例如核酸，形成解析图案。 通过首先将数字图像数据沿着与分辨方向垂直的方向划分为两个或更多个块，然后准备由垂直于分辨率的方向的位置构成的波形来确定整个数字图像数据中的分辨图案的总边界面积 通过编译每个块的数字图像日期来计算每个块的方向。 波形用于建立每个块的分辨图案的区域的边界，并且内插每个块的分辨图案区域的边界以建立分辨图案的总边界面积。 该方法是一种易于进行，准确的分析方法，用于获得位置信息，从而获得各种放射性标记物质的身份。

公开(公告)号：US07365853B2

公开(公告)日：2008-04-29

申请号：US11239305

申请日：2005-09-30

申请人： Shu Sato ,  Toshihito Kimura ,  Hisashi Ohtsuka

发明人： Shu Sato ,  Toshihito Kimura ,  Hisashi Ohtsuka

IPC分类号： G01N21/55

CPC分类号： G01N21/553

摘要： A measurement path is filled with air prior to performing actual measurement. A p-polarized light beam is caused to enter an interface, and the intensity distribution of the light beam reflected at the interface is detected by a photodiode array to obtain a reference intensity distribution of the light beam itself. Thereafter, the measurement path is filled with a target for measurement, and the intensity distribution of a light beam reflected at the interface is measured. Each of the measured distribution values are divided by the reference intensity distribution, to cancel out influences due to fluctuations in the intensity distribution of the light beam. Thereby, the position of an attenuated total reflection angle is detected with high accuracy. Because a light beam constituted by p-polarized light waves is utilized, separating means for separating the light beam reflected at the interface into p-polarized and s-polarized light waves becomes unnecessary.

摘要翻译： 在执行实际测量之前，测量路径充满空气。 使p偏振光束进入界面，并且通过光电二极管阵列检测在界面处反射的光束的强度分布，以获得光束本身的参考光强分布。 此后，用测量目标填充测量路径，并且测量在界面处反射的光束的强度分布。 每个测量分布值除以参考强度分布，以消除由于光束的强度分布的波动引起的影响。 从而，以高精度检测衰减的全反射角度的位置。 由于利用由p偏振光波构成的光束，所以不需要用于将在界面处反射的光束分离为p偏振光和S偏振光波的分离装置。