公开(公告)号：US3591480A

公开(公告)日：1971-07-06

申请号：US3591480D

申请日：1968-07-15

Applicant: IBM

Inventor： NEFF GORDON W ,  SAMBUCETTI CARLOS J ,  IOMKO JOHN E

IPC: G01N33/66 ,  C12M1/34 ,  C12Q1/00 ,  G01N27/327 ,  G01N27/42 ,  G01N27/49 ,  G01N33/487 ,  G01N27/32 ,  G01N27/40

CPC classification number: C12Q1/006 ,  G01N27/3271 ,  Y10T436/144444 ,  Y10T436/255

Abstract: THEN A PLATINUM AND CALOMEL ELECTRODE, COUPLED VIA AN ELECTROLYTE SOLUTION, ACT TO CAUSE GALVANIC COULOMETRIC REDUCTION OF THE IODINE TO REGENERATE IODIDE. AT STEADY STATE THE ONLY CURRENT-REGULATING MECHANISM IS THE DIFFUSION OF GLUCOSE ACROSS THE MEMBRANE, WHICH IS A LINEAR FUNCTION OF CONCENTRATION.

A MEASURING SYSTEM IS PROVIDED WHICH DIRECTLY MEASURES THE CONCENTRATION OF GLUCOSE IN BIOLOGICAL FLUIDS. GLUCOSE FROM A BIOLOGICAL FLUID IS DIFFUSED THROUGH A SEMIPERMEABLE MEMBRANE INTO A REACTION CHAMBER AND IS CATALYZED BY THE ENZYME GLUCOSE OXIDASE WHEREUPON GLUCONIC ACID AND HYDROGEN PEROXIDE ARE FORMED. IODIDE IONS IN THE REACTION CHAMBER THEN EFFECT THE DECOMPOSITION OF THE HYDROGEN PEROXIDE TO FORM IODINE AND WATER.