公开(公告)号：US20210317445A1

公开(公告)日：2021-10-14

申请号：US16945575

申请日：2020-07-31

Applicant: Inscripta, Inc.

Inventor： Andrea HALWEG-EDWARDS ,  Joshua SHORENSTEIN ,  Andrew GARST ,  Craig STRUBLE ,  Miles GANDER ,  Juhan KIM ,  Bryan LELAND ,  Eileen SPINDLER ,  Paul HARDENBOL

IPC: C12N15/10

Abstract: The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.

公开(公告)号：US20230183683A1

公开(公告)日：2023-06-15

申请号：US18166868

申请日：2023-02-09

Applicant: Inscripta, Inc.

Inventor： Tian TIAN ,  Eileen SPINDLER ,  Clint DAVIS ,  Charles JOHNSON ,  Andrew GARST

IPC: C12N15/10 ,  C12N15/11 ,  C12N9/22 ,  C12N15/63 ,  C12N15/70

CPC classification number: C12N15/1082 ,  C12N15/111 ,  C12N9/22 ,  C12N15/63 ,  C12N15/70 ,  C12N2310/20 ,  C12N2820/002

Abstract: The present disclosure provides compositions of matter, methods, modules and automated multi-module instrumentation for performing editing of live cells followed by curing of editing and engine vectors from prior rounds of editing, followed by curing of the curing vector.

公开(公告)号：US20220246235A1

公开(公告)日：2022-08-04

申请号：US17726250

申请日：2022-04-21

Applicant: Inscripta, Inc.

Inventor： Andrea HALWEG-EDWARDS ,  Joshua SHORENSTEIN ,  Andrew GARST ,  Craig STRUBLE ,  Miles GANDER ,  Juhan KIM ,  Bryan LELAND ,  Eileen SPINDLER ,  Paul HARDENBOL ,  Aaron BROOKS ,  Eric ABBATE

IPC: G16B20/30 ,  G16B40/20 ,  C12N15/10 ,  G06N3/08

Abstract: The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.

公开(公告)号：US20230295643A1

公开(公告)日：2023-09-21

申请号：US18182864

申请日：2023-03-13

Applicant: Inscripta, Inc.

Inventor： Charles Johnson ,  Tian TIAN ,  Eileen SPINDLER

IPC: C12N15/70 ,  C12N15/113 ,  C12N9/22

CPC classification number: C12N15/70 ,  C12N15/113 ,  C12N9/22 ,  C12N2800/80 ,  C12N2310/20

Abstract: The present disclosure provides automated multi-module instrumentation and automated methods for performing recursive editing of live cells with curing of editing vectors from prior rounds of editing.

公开(公告)号：US20210317444A1

公开(公告)日：2021-10-14

申请号：US16903324

申请日：2020-06-16

Applicant: Inscripta, Inc.

Inventor： Andrea HALWEG-EDWARDS ,  Joshua SHORENSTEIN ,  Andrew GARST ,  Craig STRUBLE ,  Miles GANDER ,  Juhan KIM ,  Bryan LELAND ,  Eileen SPINDLER ,  Paul HARDENBOL

IPC: C12N15/10

Abstract: The present disclosure is drawn to creating cassette designs for nucleic acid-guided nuclease editing. In designing editing cassettes, a set of edit specifications must first be obtained. These edit specifications are taken together with a set of configuration parameters to start a computational pipeline that generates a collection of cassette designs. The process of designing editing cassettes involves the following exemplary steps: 1) creation of a set of candidate cassette designs for each unique edit specification, 2) enumeration of features describing biophysical characteristics of each candidate design, 3) providing each candidate design with a score, and 4) returning a number of scored and rank-ordered candidate cassette designs for each edit specification.

公开(公告)号：US20230112702A1

公开(公告)日：2023-04-13

申请号：US17961763

申请日：2022-10-07

Applicant: Inscripta, Inc.

Inventor： Tian TIAN ,  Patrick WESTFALL ,  Eileen SPINDLER ,  Juhan KIM

IPC: C12N15/113 ,  C12N15/11 ,  C12N15/63 ,  C12N15/70 ,  C12N9/22

Abstract: The present disclosure relates to methods, compositions, and automated multi-module cell processing instruments for modulation of gene utilizing nuclease-mediated systems, and in particular, inactive (“dead”) nuclease-mediated CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) systems.