公开(公告)号：US20230136423A1

公开(公告)日：2023-05-04

申请号：US17975844

申请日：2022-10-28

Applicant: Inscripta, Inc.

Inventor： Patrick WESTFALL ,  Catherine BARANOWSKI

IPC: C12N15/10 ,  C12N9/22 ,  C12N15/11 ,  C12N15/72 ,  C12N1/20

Abstract: The present disclosure relates to methods and compositions for nuclease-mediated plasmid integration into the genome of a population of live cells, as well as automated multi-module instruments for performing these methods and using these compositions.

公开(公告)号：US20230112702A1

公开(公告)日：2023-04-13

申请号：US17961763

申请日：2022-10-07

Applicant: Inscripta, Inc.

Inventor： Tian TIAN ,  Patrick WESTFALL ,  Eileen SPINDLER ,  Juhan KIM

IPC: C12N15/113 ,  C12N15/11 ,  C12N15/63 ,  C12N15/70 ,  C12N9/22

Abstract: The present disclosure relates to methods, compositions, and automated multi-module cell processing instruments for modulation of gene utilizing nuclease-mediated systems, and in particular, inactive (“dead”) nuclease-mediated CRISPR interference (CRISPRi) and CRISPR activation (CRISPRa) systems.