公开(公告)号：US20210130834A1

公开(公告)日：2021-05-06

申请号：US17140056

申请日：2021-01-02

Applicant: Inscripta, Inc.

Inventor： Krishna Kannan ,  Miles Gander ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/80 ,  C12N15/10

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US11566241B2

公开(公告)日：2023-01-31

申请号：US17492435

申请日：2021-10-01

Applicant: Inscripta, Inc.

Inventor： Andrea Halweg-Edwards ,  Thomas Hraha ,  Krishna Yerramsetty ,  Shea Lambert ,  Miles Gander ,  Matthew David Estes ,  Chad Douglas Sanada ,  Isaac David Wagner ,  Paul Hardenbol

IPC: C12N15/10 ,  G16B35/20 ,  G16B40/20 ,  G16B35/10

Abstract: Disclosed systems and methods relate to predicting the relative representation of genomic variants in an edited cell population, based on the editing cassette design representation in an editing cassette design library used to generate the edited cell population. A library of editing cassette designs is generated, and a feature vector, or sequence embedding, is developed for each design using natural language processing techniques. The feature vector may be based upon sequence attributes and editing kinetics of each cassette design as well as attributes that describe the library context. Features may include sequence embeddings generated from a neural network, linguistic-type distances, and statistical distance summaries thereof. The feature vectors are classified using one or more machine learning models, and the classified feature vectors are used to predict the representation of each design an edited cell population.

公开(公告)号：US11066675B2

公开(公告)日：2021-07-20

申请号：US17140056

申请日：2021-01-02

Applicant: Inscripta, Inc.

Inventor： Krishna Kannan ,  Miles Gander ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/80 ,  C12N15/10

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US10927385B2

公开(公告)日：2021-02-23

申请号：US16904405

申请日：2020-06-17

Applicant: Inscripta, Inc.

Inventor： Krishna Kannan ,  Miles Gander ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/80 ,  C12N15/10

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US20210317464A1

公开(公告)日：2021-10-14

申请号：US17355081

申请日：2021-06-22

Applicant: Inscripta, Inc.

Inventor： Krishna Kannan ,  Miles Gander ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/80 ,  C12N15/10

Abstract: The present disclosure provides methods to increase the percentage of edited yeast cells in a cell population using nucleic-acid guided editing, and automated multi-module instruments for performing these methods.

公开(公告)号：US20210246441A1

公开(公告)日：2021-08-12

申请号：US17167018

申请日：2021-02-03

Applicant: Inscripta, Inc.

Inventor： Paul Hardenbol ,  Kara Juneau ,  Michael Graige

IPC: C12N15/10 ,  C12Q1/6876

Abstract: The present disclosure relates to methods and compositions to enable use of editing cassettes for nucleic-acid guided editing and then repurposing the cassettes to increase targeted sequencing efficiency and reduce sequencing costs of the edited genomes.

公开(公告)号：US20190292568A1

公开(公告)日：2019-09-26

申请号：US16358461

申请日：2019-03-19

Applicant: Inscripta, Inc.

Inventor： Christopher Abraham ,  Charles Johnson ,  Clint Davis ,  Eileen Spindler ,  Paul Hardenbol

IPC: C12N15/90 ,  C12N15/113 ,  C12N9/22

Abstract: The present disclosure thus provides methods for higher efficiency cell editing that maintain the viability of the cells. In certain aspects, the present disclosure provides systems and instruments for automated methods of multiplexed nuclease-directed genome editing using nuclease expression under a constitutive promoter. In specific aspects, the constitutive promoter is an insulated promoter. In other aspects, the constitutive promoter is a heterologous promoter. In still other aspects, the constitutive promoter is a synthetic promoter.