公开(公告)号：US20220349091A1

公开(公告)日：2022-11-03

申请号：US17864744

申请日：2022-07-14

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C12N15/85 ,  C07K16/28 ,  C40B30/04 ,  C12N9/22 ,  C40B40/10 ,  C40B40/02 ,  C40B70/00 ,  C07K14/74 ,  C12N15/10 ,  C40B20/04 ,  C12N15/90 ,  C12N15/81

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US11473214B2

公开(公告)日：2022-10-18

申请号：US17728135

申请日：2022-04-25

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C12N15/90 ,  C12N15/10 ,  C40B40/10 ,  C12N15/85 ,  C07K16/28 ,  C40B70/00 ,  C40B40/02 ,  C07K14/74 ,  C12N15/81 ,  C12N9/22 ,  C40B20/04 ,  C40B30/04

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US20200063120A1

公开(公告)日：2020-02-27

申请号：US16670396

申请日：2019-10-31

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/10 ,  C12N15/90 ,  C12N9/22 ,  C12N15/81

Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

公开(公告)号：US20190323001A1

公开(公告)日：2019-10-24

申请号：US16393199

申请日：2019-04-24

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/10 ,  C40B40/02

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of binding pairs. The engineered peptides may be displayed using various cell surface display technologies.

公开(公告)号：US11555184B2

公开(公告)日：2023-01-17

申请号：US16988696

申请日：2020-08-09

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/19 ,  C12N15/10 ,  C12N15/90 ,  C12N9/22 ,  C12N15/81 ,  C40B50/04 ,  C40B40/02

Abstract: The present disclosure methods for identifying binding partners using cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of peptides that bind to targets of interest. The engineered peptides are preferably expressed in the cells under conditions that provide both secretion and display of the engineered peptides on the cell surfaces, thus providing access of the engineered peptides to identify potential binding pairs. The cell libraries cab be engineered using an automated editing system that provides for one or more targeted edits per cell.

公开(公告)号：US11293117B2

公开(公告)日：2022-04-05

申请号：US17016454

申请日：2020-09-10

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C07K14/74 ,  C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C12N15/10 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C40B70/00

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US11198857B2

公开(公告)日：2021-12-14

申请号：US17388358

申请日：2021-07-29

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US11104890B1

公开(公告)日：2021-08-31

申请号：US17230765

申请日：2021-04-14

Applicant: Inscripta, Inc.

Inventor： Aamir Mir ,  Andrew Garst ,  Stephen Federowicz ,  Kyle Seamon

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/70 ,  C12N15/62

Abstract: The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions.

公开(公告)号：US20200318256A1

公开(公告)日：2020-10-08

申请号：US16904640

申请日：2020-06-18

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C40B50/06 ,  C40B70/00 ,  C40B40/10 ,  C12N15/90 ,  C12N15/85 ,  C40B20/04 ,  C12N15/81 ,  C12N15/10 ,  C12N9/22 ,  C07K16/28 ,  C40B40/02 ,  C07K14/74 ,  C40B30/04

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.

公开(公告)号：US10676842B2

公开(公告)日：2020-06-09

申请号：US16670340

申请日：2019-10-31

Applicant: Inscripta, Inc.

Inventor： Stephen Federowicz ,  Deanna Church ,  Michael Graige

IPC: C12N15/10 ,  C40B50/06 ,  C40B70/00 ,  C40B40/02 ,  C40B20/04 ,  C12N15/90 ,  C12N15/85 ,  C12N9/22 ,  C07K14/74 ,  C07K16/28 ,  C40B40/10 ,  C40B30/04 ,  C12N15/81

Abstract: The present disclosure provides instrumentation and automated methods for creating cell surface display libraries, where the cells of the library display engineered peptides on their cell surfaces for identification of antigens that bind to T-cell receptors. The engineered peptides may be putative antigens or binding regions of the T-cell receptors.