公开(公告)号：US20220073706A1

公开(公告)日：2022-03-10

申请号：US17524801

申请日：2021-11-12

Applicant: Jiangnan University

Inventor： Ming MIAO ,  Xue JIA ,  Osvaldo H. Campanella ,  Zhengyu JIN ,  Tao ZHANG ,  Lei YE

IPC: C08L3/08 ,  C08B31/04 ,  C08B31/10 ,  C08B31/00 ,  C08L3/06 ,  C08J5/18 ,  A61K47/36 ,  B65D65/46

Abstract: Disclosed is a processing method of a self-reinforced starch-based multifunctional material, and belongs to the technical field of starch deep processing. The processing method takes bulk starch as a base material, including the following steps: firstly reacting starch nanoparticles with an organic acid anhydride reagent and adding a bacteriostatic agent to prepare composite nanoparticles, then mixing the composite nanoparticles with the bulk starch, an etherifying agent, a crosslinking agent, a plasticizer and the like, and finally preparing a starch-based multifunctional material by dry extrusion modification combined with a starch-based nanoparticle assembly and reinforcement technology. The method of the disclosure is simple and convenient in step, mild and controllable in reaction, and continuous and green in production. The obtained product has good mechanical properties, high barrier properties and high antibacterial properties, can be applied to many fields such as food, textiles, daily chemicals and medicine, and has a broad market prospect.

公开(公告)号：US20200109391A1

公开(公告)日：2020-04-09

申请号：US16718743

申请日：2019-12-18

Applicant: Jiangnan University

Inventor： Tao ZHANG ,  Bo JIANG ,  Hangyu JIANG ,  Wanmeng MU ,  Ming MIAO

IPC: C12N9/78 ,  C12P13/10 ,  A61P35/02 ,  C12N15/70 ,  A61K38/50

Abstract: An arginine deiminase mutant with improved enzyme activity and temperature stability and application thereof were provided, belonging to the technical field of genetic engineering and enzyme engineering. The arginine deiminase mutant is proline, namely Gly292 Pro, mutated from glycine near an enzyme active center. A wild-type arginine deiminase arcA coding gene is molecularly modified by a site-directed mutation technique to obtain a mutant enzyme ADIG292P, which has glycine at position 292 of an amino acid sequence of the wild type arginine deiminase mutated to proline. The arginine deiminase, modified by site-directed mutation, of the present invention has 1.5 times of increase in enzyme activity and 5.43 times of increase in half-life period at 40° C. compared with the wild-type enzyme, which solves the problems of low catalytic ability and temperature stability during the catalytic synthesis of citrulline using arginine deiminase, and lays a foundation for industrial production of efficient synthesis of citrulline and medication application.

公开(公告)号：US20190239553A1

公开(公告)日：2019-08-08

申请号：US16368429

申请日：2019-03-28

Applicant: Jiangnan University

Inventor： Ming MIAO ,  Bo JIANG ,  Yang QI ,  Zhengyu JIN ,  Chen CHEN ,  Tao ZHANG

IPC: A23P10/30 ,  A23L33/105 ,  A23L33/12 ,  C12P19/04 ,  C12P19/18

CPC classification number: A23P10/30 ,  A23L33/105 ,  A23L33/12 ,  A23V2002/00 ,  A23V2200/224 ,  A23V2200/30 ,  A23V2250/1872 ,  A23V2250/5118 ,  C12P19/04 ,  C12P19/18 ,  C12Y204/01001

Abstract: The present invention discloses a glucan-based shell-core structure carrier material and preparation and application thereof, and belongs to the technical field of modern food processing. Spherical hyperbranched water-soluble amylum grains are used as the raw material, and an enzymatic grafting and chain extending process is adopted for treatment to modify the surfaces of water-soluble glucan molecules into a firm shell structure with densely cumulated crystal structures, and form the glucan-based carrying material with the shell-core structure of which an inner core cavity has an amorphous state and an outer shell layer has a crystalline state. The adopted spherical hyperbranched water-soluble amylum grains have wide sources of raw materials and are not limited by producing areas and seasons; the preparation has simple and convenient steps, easy operation, controllable reaction conditions, relatively low cost and basically no pollution to the environment; and the prepared product can effectively protect, deliver and release functional nutritional components, can be applied to multiple fields of food, medicine, chemicals for daily use and the like, and has great market prospects and broad economic benefits.

公开(公告)号：US20180251748A1

公开(公告)日：2018-09-06

申请号：US15907340

申请日：2018-02-28

Applicant: Jiangnan University

Inventor： Tao ZHANG ,  Bo JIANG ,  Hangyu JIANG ,  Wanmeng MU ,  Ming MIAO

IPC: C12N9/78 ,  A61K38/50 ,  A61P35/02 ,  C12N15/70 ,  C12P13/10

CPC classification number: C12N9/78 ,  A61K38/50 ,  A61P35/02 ,  C12N15/70 ,  C12P13/10 ,  C12Y305/03006

Abstract: An arginine deiminase mutant with improved enzyme activity and temperature stability and application thereof were provided, belonging to the technical field of genetic engineering and enzyme engineering. The arginine deiminase mutant is proline, namely Gly292 Pro, mutated from glycine near an enzyme active center. A wild-type arginine deiminase arcA coding gene is molecularly modified by a site-directed mutation technique to obtain a mutant enzyme ADIG292P, which has glycine at position 292 of an amino acid sequence of the wild type arginine deiminase mutated to proline. The arginine deiminase, modified by site-directed mutation, of the present invention has 1.5 times of increase in enzyme activity and 5.43 times of increase in half-life period at 40° C. compared with the wild-type enzyme, which solves the problems of low catalytic ability and temperature stability during the catalytic synthesis of citrulline using arginine deiminase, and lays a foundation for industrial production of efficient synthesis of citrulline and medication application.

公开(公告)号：US20200277408A1

公开(公告)日：2020-09-03

申请号：US16875048

申请日：2020-05-15

Applicant: Jiangnan University

Inventor： Ming MIAO ,  Bo JIANG ,  Chen CHEN ,  Yao LIU ,  Yuqi YANG ,  Tao ZHANG

IPC: C08B30/12 ,  C12P19/14

Abstract: The present disclosure discloses an amylopectin-based cyclic glucan and the processing method for the same, and belongs to the technical field of food processing. The present disclosure uses a starch as a raw material, prepares an amylopectin-based cyclic glucan through sugar chain degradation grading and glycosidase-catalyzed trans-glycoside technology, and can be used as a steady-state carrier material for food active factors. The method of the disclosure has advantages of green environmental protection, high processing yield and low cost. The prepared product has high branching degree, special large ring structure and good water solubility, and can be used for steady-state delivery and active protection of natural functional substances, involving nutritional food, medicine, daily chemicals and other fields.

公开(公告)号：US20190136219A1

公开(公告)日：2019-05-09

申请号：US16245881

申请日：2019-01-11

Applicant: Jiangnan University

Inventor： Tao ZHANG ,  Bo JIANG ,  Hangyu JIANG ,  Wanmeng MU ,  Ming MIAO

IPC: C12N9/78 ,  C12P13/10 ,  C12Q1/34

CPC classification number: C12N9/78 ,  C12N2800/101 ,  C12P13/10 ,  C12Q1/34 ,  C12Y305/03006

Abstract: A genetically engineered arginine deiminase reconstructed by site-directed mutagenesis belongs to the technical field of genetic engineering technology. Its amino acid sequence is shown as SEQ ID No. 1. In the amino acid sequence of the arginine deiminase reconstructed by site-directed mutagenesis, glycine at position 264 is mutated to proline, compared to an amino acid sequence of native arginine deiminase. Compared with wild type enzyme, the effective pH range effect of the mutated arginine deiminase according to the present invention is broadened to a certain extent, and especially a good enzyme activity is achieved at physiological pH 7.4. With the broadening of the effective pH effect range, the mutant enzyme still has higher stability under the condition of pH 5.5-7.5. Therefore, the problem that the arginine deiminase generally is low in enzymatic activity and short in half-life in vivo under physiological conditions in clinical application for tumor therapy is solved, and a good condition for using the enzyme and an encoding gene thereof for clinical treatment is created.

公开(公告)号：US20240260589A1

公开(公告)日：2024-08-08

申请号：US18641553

申请日：2024-04-22

Applicant: Jiangnan University

Inventor： Long CHEN ,  Yuanhui CHEN ,  Zhengyu JIN ,  Yaoqi TIAN ,  Ming MIAO ,  Jianwei ZHAO

IPC: A21D8/04 ,  A21D2/26 ,  A21D2/30 ,  A21D6/00

CPC classification number: A21D8/042 ,  A21D2/261 ,  A21D2/30 ,  A21D6/003

Abstract: Disclosed are a preparation method and application of flour with low oil absorption during frying, belonging to the field of flour modification. The preparation method of flour with low oil absorption during frying increases the cross-linking degree of starch, protein, and starch-protein in the flour by adding glutamine transaminase first and then phytic acid or adding phytic acid first and then glutamine transaminase so as to improve the compactness of the flour structure, which reduces water evaporation and oil absorption during frying after the flour is made into dough, and thus ultimately forms the modified flour with low oil absorption during frying. The method is simple, green, pollution-free, and low in energy consumption, and the modified flour prepared has improved thermal stability and reduced oil absorption after frying. The present disclosure has broad application prospects in the fields of flour modification and fried foods.

公开(公告)号：US20220273004A1

公开(公告)日：2022-09-01

申请号：US17744811

申请日：2022-05-16

Applicant: Jiangnan University

Inventor： Ming MIAO ,  Yao LIU ,  Chaohui ZHI ,  Tao ZHANG ,  Zhengyu JIN

IPC: A23L3/3463 ,  A23L3/3571 ,  A61Q17/00 ,  B82Y5/00

Abstract: The present disclosure discloses an antibacterial glucose-based composite nanoparticle and a processing method and use thereof, and belongs to the technical field of processing of modern food. According to the present disclosure, the antibacterial composite nanoparticle is prepared by using a particle surface positioning modification technology and a physical field charge transfer technology with a natural glucose-based nanoparticle as a raw material. The obtained antibacterial composite nanoparticle has a particle size of 50-1,000 nm, a surface zeta potential of 0 to −10 mV and a broad-spectrum antibacterial rate of greater than 98%. The shelf life of food can be effectively prolonged to prevent spoilage of products. The antibacterial composite nanoparticle can be used in food, textiles, daily chemicals, medicine and many other fields, and has a wide application prospect.

公开(公告)号：US20210368809A1

公开(公告)日：2021-12-02

申请号：US17403932

申请日：2021-08-17

Applicant: Jiangnan University

Inventor： Ming MIAO ,  Xiao ZHOU ,  Yungao LI ,  Yuqi YANG ,  Tao ZHANG ,  Zhengyu JIN ,  Zhiyuan LIU

IPC: A21D2/36 ,  A23L7/109 ,  A21D13/047 ,  A21D10/04 ,  A23L33/185 ,  A23L33/105

Abstract: The disclosure discloses a nutritional cereal-based food with low glycemic load and processing method thereof, and belongs to the technical field of processing of healthy foods. According to the disclosure, bulk grain crops are used as raw materials and mixed with a natural plant extract with the content of 3-deoxyanthocyanidin more than 3 mg/100 g and a sulfur-rich plant or animal protein, so that in-situ encapsulation of starch granules is achieved through an interaction of a sulfur-containing protein and 3-deoxyanthocyanidin, and a grain-source nutritional food for special dietary uses with low GI. According to the method of the disclosure, not only is the process environmentally friendly, operation procedures are simple, but also slow release of blood glucose after a meal is achieved by using the product, and the product can be used as a meal replacement food for people with abnormal glucose metabolism and the like.