公开(公告)号：US20230265411A1

公开(公告)日：2023-08-24

申请号：US18187984

申请日：2023-03-22

Applicant: Jiangnan University

Inventor： Tao ZHANG ,  Mengying Hu ,  Bo Jiang ,  Mengli Li

IPC: C12N9/90 ,  C12N15/75

CPC classification number: C12N9/90 ,  C12N15/75

Abstract: The present disclosure discloses a strain producing D-allulose 3-epimerase and application thereof, and belongs to the technical field of bioengineering. The present disclosure provides a method for improving the expression of D-allulose 3-epimerase by screening promoters and optimizing RBS thereof. The recombinant Bacillus subtilis constructed using thevectors pP43NMK-hag and pP43NMK-hag-RBS4 provided by the present disclosure improves the enzyme activity of a target gene D-allulose 3-epimerase, and theenzyme activities in shake flasks upon transformation are 1.30 times and 1.69 times that of an original vector. The present disclosure further provides a non-antibiotic resistance vector and a non-antibiotic resistance recombinant B. subtilis strain. Using the non-antibiotic resistance strain B. subtilis 1A751-dal-/pP43NMK-hag-RBS4-dpe-dal provided by the present disclosure, the highest fermentation enzyme activity in a shake flask is 24.72 U/mL, and the enzyme activity in a fermenter is 714.8 U/mL.

公开(公告)号：US20220073706A1

公开(公告)日：2022-03-10

申请号：US17524801

申请日：2021-11-12

Applicant: Jiangnan University

Inventor： Ming MIAO ,  Xue JIA ,  Osvaldo H. Campanella ,  Zhengyu JIN ,  Tao ZHANG ,  Lei YE

IPC: C08L3/08 ,  C08B31/04 ,  C08B31/10 ,  C08B31/00 ,  C08L3/06 ,  C08J5/18 ,  A61K47/36 ,  B65D65/46

Abstract: Disclosed is a processing method of a self-reinforced starch-based multifunctional material, and belongs to the technical field of starch deep processing. The processing method takes bulk starch as a base material, including the following steps: firstly reacting starch nanoparticles with an organic acid anhydride reagent and adding a bacteriostatic agent to prepare composite nanoparticles, then mixing the composite nanoparticles with the bulk starch, an etherifying agent, a crosslinking agent, a plasticizer and the like, and finally preparing a starch-based multifunctional material by dry extrusion modification combined with a starch-based nanoparticle assembly and reinforcement technology. The method of the disclosure is simple and convenient in step, mild and controllable in reaction, and continuous and green in production. The obtained product has good mechanical properties, high barrier properties and high antibacterial properties, can be applied to many fields such as food, textiles, daily chemicals and medicine, and has a broad market prospect.

公开(公告)号：US20210403894A1

公开(公告)日：2021-12-30

申请号：US17463628

申请日：2021-09-01

Applicant: Jiangnan University

Inventor： Bo JIANG ,  Tao ZHANG ,  Qing MENG ,  Jingjing CHEN

IPC: C12N9/88 ,  C12N1/20

Abstract: The disclosure discloses an alginate lyase and application thereof, and belongs to the technical field of biology. The alginate lyase provided by the disclosure has high degradation activity, and the enzyme activity reaches 65 U/mg; the alginate lyase is stable in nature, and the enzyme activity remains 98% or higher of the initial enzyme activity after storage at 4° C. for 18 months; and the alginate lyase has high product specificity. The disclosure uses E. coli as a host to express the alginate lyase derived from V. natriegens, the obtained recombinant E. coli can produce the alginate lyase secreted extracellularly in a conventional LB medium without adding an induction substrate sodium alginate, so the downstream processing technology of protein is simplified, and the disclosure has great industrial application potential.

公开(公告)号：US20190239553A1

公开(公告)日：2019-08-08

申请号：US16368429

申请日：2019-03-28

Applicant: Jiangnan University

Inventor： Ming MIAO ,  Bo JIANG ,  Yang QI ,  Zhengyu JIN ,  Chen CHEN ,  Tao ZHANG

IPC: A23P10/30 ,  A23L33/105 ,  A23L33/12 ,  C12P19/04 ,  C12P19/18

CPC classification number: A23P10/30 ,  A23L33/105 ,  A23L33/12 ,  A23V2002/00 ,  A23V2200/224 ,  A23V2200/30 ,  A23V2250/1872 ,  A23V2250/5118 ,  C12P19/04 ,  C12P19/18 ,  C12Y204/01001

Abstract: The present invention discloses a glucan-based shell-core structure carrier material and preparation and application thereof, and belongs to the technical field of modern food processing. Spherical hyperbranched water-soluble amylum grains are used as the raw material, and an enzymatic grafting and chain extending process is adopted for treatment to modify the surfaces of water-soluble glucan molecules into a firm shell structure with densely cumulated crystal structures, and form the glucan-based carrying material with the shell-core structure of which an inner core cavity has an amorphous state and an outer shell layer has a crystalline state. The adopted spherical hyperbranched water-soluble amylum grains have wide sources of raw materials and are not limited by producing areas and seasons; the preparation has simple and convenient steps, easy operation, controllable reaction conditions, relatively low cost and basically no pollution to the environment; and the prepared product can effectively protect, deliver and release functional nutritional components, can be applied to multiple fields of food, medicine, chemicals for daily use and the like, and has great market prospects and broad economic benefits.

公开(公告)号：US20180251748A1

公开(公告)日：2018-09-06

申请号：US15907340

申请日：2018-02-28

Applicant: Jiangnan University

Inventor： Tao ZHANG ,  Bo JIANG ,  Hangyu JIANG ,  Wanmeng MU ,  Ming MIAO

IPC: C12N9/78 ,  A61K38/50 ,  A61P35/02 ,  C12N15/70 ,  C12P13/10

CPC classification number: C12N9/78 ,  A61K38/50 ,  A61P35/02 ,  C12N15/70 ,  C12P13/10 ,  C12Y305/03006

Abstract: An arginine deiminase mutant with improved enzyme activity and temperature stability and application thereof were provided, belonging to the technical field of genetic engineering and enzyme engineering. The arginine deiminase mutant is proline, namely Gly292 Pro, mutated from glycine near an enzyme active center. A wild-type arginine deiminase arcA coding gene is molecularly modified by a site-directed mutation technique to obtain a mutant enzyme ADIG292P, which has glycine at position 292 of an amino acid sequence of the wild type arginine deiminase mutated to proline. The arginine deiminase, modified by site-directed mutation, of the present invention has 1.5 times of increase in enzyme activity and 5.43 times of increase in half-life period at 40° C. compared with the wild-type enzyme, which solves the problems of low catalytic ability and temperature stability during the catalytic synthesis of citrulline using arginine deiminase, and lays a foundation for industrial production of efficient synthesis of citrulline and medication application.

公开(公告)号：US20230279456A1

公开(公告)日：2023-09-07

申请号：US18061585

申请日：2022-12-05

Applicant: Jiangnan University

Inventor： Tao ZHANG ,  Bo JIANG ,  Miaomiao HU ,  Mengli LI

IPC: C12P19/26 ,  C12N15/70 ,  C12N9/10 ,  C12N9/12 ,  C12N9/38 ,  C12N9/90

CPC classification number: C12P19/26 ,  C12N15/70 ,  C12N9/1051 ,  C12N9/1205 ,  C12N9/1241 ,  C12N9/2471 ,  C12N9/90 ,  C12Y204/01038 ,  C12Y204/01222 ,  C12Y207/01006 ,  C12Y501/03002 ,  C12Y504/02002 ,  C12Y207/07012

Abstract: The disclosure discloses genetically engineered bacteria producing lacto-N-neotetraose and a production method thereof, and belongs to the fields of metabolic engineering and food biotechnology. To solve the problem of low yield of lacto-N-neotetraose produced by a microbial method in the prior art, through exogenous expression of lgtA and lgtB, reasonable combination and regulation of overexpression of lacY, pgm, galE, galT and galK in a lacto-N-neotetraose synthesis pathway, knockout of lacZ expression in an Escherichia coli host, and optimization of a carbon source in the culture process, the disclosure achieves the objectives of regulating the carbon flux of a metabolic pathway and improving the yield of lacto-N-neotetraose. In a shake flask experiment, the yield of lacto-N-neotetraose produced by E. coli increased from 304 mg/L to 1031 mg/L, laying a foundation for industrial production of the lacto-N-neotetraose.

公开(公告)号：US20220273004A1

公开(公告)日：2022-09-01

申请号：US17744811

申请日：2022-05-16

Applicant: Jiangnan University

Inventor： Ming MIAO ,  Yao LIU ,  Chaohui ZHI ,  Tao ZHANG ,  Zhengyu JIN

IPC: A23L3/3463 ,  A23L3/3571 ,  A61Q17/00 ,  B82Y5/00

Abstract: The present disclosure discloses an antibacterial glucose-based composite nanoparticle and a processing method and use thereof, and belongs to the technical field of processing of modern food. According to the present disclosure, the antibacterial composite nanoparticle is prepared by using a particle surface positioning modification technology and a physical field charge transfer technology with a natural glucose-based nanoparticle as a raw material. The obtained antibacterial composite nanoparticle has a particle size of 50-1,000 nm, a surface zeta potential of 0 to −10 mV and a broad-spectrum antibacterial rate of greater than 98%. The shelf life of food can be effectively prolonged to prevent spoilage of products. The antibacterial composite nanoparticle can be used in food, textiles, daily chemicals, medicine and many other fields, and has a wide application prospect.

公开(公告)号：US20210368809A1

公开(公告)日：2021-12-02

申请号：US17403932

申请日：2021-08-17

Applicant: Jiangnan University

Inventor： Ming MIAO ,  Xiao ZHOU ,  Yungao LI ,  Yuqi YANG ,  Tao ZHANG ,  Zhengyu JIN ,  Zhiyuan LIU

IPC: A21D2/36 ,  A23L7/109 ,  A21D13/047 ,  A21D10/04 ,  A23L33/185 ,  A23L33/105

Abstract: The disclosure discloses a nutritional cereal-based food with low glycemic load and processing method thereof, and belongs to the technical field of processing of healthy foods. According to the disclosure, bulk grain crops are used as raw materials and mixed with a natural plant extract with the content of 3-deoxyanthocyanidin more than 3 mg/100 g and a sulfur-rich plant or animal protein, so that in-situ encapsulation of starch granules is achieved through an interaction of a sulfur-containing protein and 3-deoxyanthocyanidin, and a grain-source nutritional food for special dietary uses with low GI. According to the method of the disclosure, not only is the process environmentally friendly, operation procedures are simple, but also slow release of blood glucose after a meal is achieved by using the product, and the product can be used as a meal replacement food for people with abnormal glucose metabolism and the like.

公开(公告)号：US20190002939A1

公开(公告)日：2019-01-03

申请号：US15777932

申请日：2016-04-15

Applicant: Jiangnan University

Inventor： Wanmeng MU ,  Bo JIANG ,  Shuhuai YU ,  Yingying ZHU ,  Tao ZHANG

IPC: C12P19/12 ,  C12P19/14

CPC classification number: C12P19/12 ,  C12N9/1051 ,  C12N9/88 ,  C12P19/14 ,  C12P19/18 ,  C12Y204/01009 ,  C12Y402/02017 ,  C12Y402/02018

Abstract: The invention discloses a high-efficiency synthesis method of difructose anhydride III. The method comprises the following steps: firstly converting sucrose into inulin by using inulosucrase without separating polysaccharide, and then converting inulin by using inulin fructotransferase to synthesize the functional disaccharide difructose anhydride III. The method has the advantages of simple process and high efficiency, and a conversion rate of synthesizing inulin into difructose anhydride III can reach 40%-54%. In order to obtain purer difructose anhydride III, yeast is utilized to remove small molecule monosaccharides in a reaction solution, and the finally obtained purer difructose anhydride III can be easily separated and purified. Thus, the method has broad market application prospects.

公开(公告)号：US20230078437A1

公开(公告)日：2023-03-16

申请号：US17990793

申请日：2022-11-21

Applicant: Jiangnan University

Inventor： Bo JIANG ,  Ying PAN ,  Tao ZHANG ,  Jingjing CHEN

IPC: A23C9/156 ,  A23L33/00 ,  A23C9/158 ,  A23C9/152 ,  A23C1/04 ,  A23L33/115 ,  A23L33/125 ,  A23L33/16 ,  A23L33/155 ,  A23L33/19

Abstract: The present disclosure discloses a milk powder containing maltopentaose trehalose instead of maltodextrin and a preparation method thereof, and belongs to the field of processing of dairy products. In the present disclosure, a formula milk powder containing maltopentaose trehalose instead of maltodextrin includes the following raw materials in parts by mass: 85-90 parts of fresh milk, 1-2 parts of maltopentaose trehalose, 5-10 parts of vegetable oil, 1-2 parts of a desalted whey powder, 1.5-3 parts of a concentrated whey protein powder, 0.5-0.8 parts of lactose, 0.05-0.1 parts of a multivitamin, and 0.05-0.1 parts of a complex mineral. Differences in physical and chemical properties of products due to a nonuniform polymerization degree of maltodextrin are reduced, and the problem that a Maillard reaction is likely to be induced when the maltodextrin is mixed with an amino acid or protein is solved.