公开(公告)号：US20230122979A1

公开(公告)日：2023-04-20

申请号：US17758659

申请日：2021-01-15

Applicant: JUMPCODE GENOMICS, INC.

Inventor： Keith BROWN

IPC: C12N15/10 ,  C12N9/16 ,  C12Q1/37 ,  C12Q1/6806

Abstract: Provided herein are methods of normalizing a population of nucleic acid samples. Methods herein can comprise: contacting a plurality of nucleic acid samples to a normalizing agent, wherein each nucleic acid of the plurality comprises a sample-specific barcode, and wherein the normalizing agent comprises a plurality of labeled enzymes capable of binding to each sample specific barcode; contacting the product to a capture agent to capture the nucleic acids that are bound to the normalizing agent; and treating the product with a protease to release the bound nucleic acids, thereby creating a normalized library having more even representation of each nucleic acid sample than the plurality of nucleic acid samples before normalization.

公开(公告)号：US20220145359A1

公开(公告)日：2022-05-12

申请号：US17430102

申请日：2020-02-11

Applicant: JUMPCODE GENOMICS, INC.

Inventor： Keith BROWN

IPC: C12Q1/6806 ,  C12N9/22 ,  C12N15/11 ,  C12N15/10

Abstract: Disclosed herein are compositions and methods related to the elimination of a first nucleic acid and enrichment of a second nucleic acid in a sample, for example to exclude the first nucleic acid from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

公开(公告)号：US20230265528A1

公开(公告)日：2023-08-24

申请号：US18041041

申请日：2021-08-11

Applicant: JUMPCODE GENOMICS, INC.

Inventor： Keith BROWN

IPC: C12Q1/689 ,  C12Q1/6869

CPC classification number: C12Q1/689 ,  C12Q1/6869 ,  C12Q1/6806

Abstract: Disclosed herein are compositions and methods related to the elimination of a first nucleic acid and/or enrichment of a second nucleic acid in a sample, for example to exclude the first nucleic acid from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.

公开(公告)号：US20230056763A1

公开(公告)日：2023-02-23

申请号：US17758656

申请日：2021-01-15

Applicant: JUMPCODE GENOMICS, INC.

Inventor： Keith BROWN

IPC: C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6853 ,  C12N9/12

Abstract: Provided herein are methods and compositions for creating a sequencing library comprising a target nucleic acid. Methods herein can comprise: contacting a nucleic acid sample to a first population of primers, a polymerase, dNTPs, and labeled ddNTPs; performing an extension reaction thereby creating an labeled extension product; contacting the extension product to a second population of primers to create a double stranded extension product comprising the target nucleic acid; contacting the double stranded extension product to a target specific enzyme under conditions allowing cleavage of at least a subset of the double stranded extension product thereby creating a cleaved target nucleic acid; and isolating the cleaved target nucleic acid.

公开(公告)号：US20220333186A1

公开(公告)日：2022-10-20

申请号：US17761866

申请日：2020-09-25

Applicant: JUMPCODE GENOMICS, INC.

Inventor： Keith BROWN

IPC: C12Q1/6855 ,  C12Q1/6806

Abstract: Disclosed herein are methods, compositions, and systems that utilize the consensus sequencing of rolling circle amplified short templates. Methods of determining a nucleic acid sequence can include one or more steps of contacting a nucleic acid in a sample to an endonuclease to cleave a target nucleic acid; ligating the target nucleic acid sequence to form a circular target nucleic acid; hybridizing at least one primer to the circular target molecule to form amplified nucleic acid through rolling circle amplification; and performing sequence analysis of the amplified nucleic acid.

公开(公告)号：US20250084484A1

公开(公告)日：2025-03-13

申请号：US18728252

申请日：2023-01-10

Applicant: Jumpcode Genomics, Inc.

Inventor： Keith BROWN

IPC: C12Q1/6886 ,  C12Q1/6855

Abstract: Provided herein are methods and compositions for analysis of a transcriptome, for example detection of a RNA isoform, of a cell, tissue, organ, or subject.

公开(公告)号：US20240182951A1

公开(公告)日：2024-06-06

申请号：US18553167

申请日：2022-03-30

Applicant: Jumpcode Genomics, Inc.

Inventor： Keith BROWN ,  Jon ARMSTRONG ,  Azeem SIDDIQUE

IPC: C12Q1/6816 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6886

CPC classification number: C12Q1/6816 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q1/6886

Abstract: Disclosed herein are compositions and methods related to the detection and/or elimination of a first nucleic acid and enrichment of a second nucleic acid in a sample, for example to exclude the first nucleic acid from downstream analysis or sequencing, or to exclude such sequences from a downstream data set.