公开(公告)号：US11655462B2

公开(公告)日：2023-05-23

申请号：US16490515

申请日：2019-05-02

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Kyung-Jin Kim ,  Jung Ho Ahn ,  Hogyun Seo ,  Jong An Lee

IPC: C12N1/20 ,  C12N9/04 ,  C12N15/74 ,  C12P7/46

CPC classification number: C12N9/0006 ,  C12N1/20 ,  C12N15/74 ,  C12P7/46 ,  C12Y101/01037

Abstract: Disclosed are a mutant microorganism for producing succinic acid exhibiting improved activity of conversion of oxaloacetate to malate through the introduction of genes encoding a malate dehydrogenase, wherein an amino acid residue that interacts with a pyrophosphate moiety of NADH through an amide functional group of a main chain of malate dehydrogenase is glutamine (Gln), and a method of producing succinic acid using the same. The mutant microorganism producing succinic acid according to the present invention is capable of producing a high concentration of succinic acid at the highest productivity compared to other mutant microorganisms reported to date when the microorganism is cultured in a limited medium. In addition, the mutant microorganism is capable of producing succinic acid at higher productivity and product concentration through further advanced fermentation technology.