公开(公告)号：US12104192B2

公开(公告)日：2024-10-01

申请号：US17194222

申请日：2021-03-06

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Dong In Kim ,  Tong Un Chae

IPC: C12N9/88 ,  C12N1/21 ,  C12P13/00 ,  C12P13/06 ,  C12P13/08

CPC classification number: C12P13/08 ,  C12N9/88 ,  C12P13/001 ,  C12P13/06 ,  C12Y401/01014

Abstract: Disclosed is a method of preparing primary amines. More particularly, disclosed are a mutant microorganism transformed with a gene encoding a valine decarboxylase and a method of preparing primary amines from amino acids using the mutant microorganism. The method has an effect of synthesizing, in an environmentally friendly manner, primary amines as precursors for pharmaceuticals and agricultural chemicals using microorganisms, as an alternative to conventional chemical synthesis methods.

公开(公告)号：US11371068B2

公开(公告)日：2022-06-28

申请号：US16483430

申请日：2018-12-12

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Xin Rui Zhao ,  Kyeong Rok Choi

IPC: C12N1/22 ,  C12P17/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90 ,  C12N9/00 ,  C12N15/67 ,  C12P13/00

Abstract: The present invention relates to a microorganism variant having the ability to extracellularly produce heme, and more particularly to a metabolically engineered microorganism variant having the ability to extracellularly produce heme and a method of producing heme using the same. According to the present invention, heme, an organometallic compound which is increasingly used as a health food or food supplement for the treatment of porphyria, can be extracellularly secreted and produced in high yield using the microorganism variant, but not conventional chemical synthesis or enzymatic synthesis.

公开(公告)号：US10961521B2

公开(公告)日：2021-03-30

申请号：US16483426

申请日：2018-02-26

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Jung Eun Yang ,  Si Jae Park

IPC: C12N9/10 ,  C12P7/62 ,  C12N9/04

Abstract: The present invention relates to a recombinant microorganism to which a gene coding for 2-hydroxyisocaproate-CoA transferase and a gene coding for polyhydroxyalkanoate synthase are introduced and which has a potential of producing polyhydroxyalkanoate bearing an aromatic monomer or a long-chain 2-HA monomer and a method for producing polyhydroxyalkanoate bearing an aromatic monomer or a long-chain 2-HA monomer, using the recombinant microorganism. According to the present invention, a biodegradable polymer bearing an aromatic monomer or a long-chain 2-HA monomer can be produced.

公开(公告)号：US10683512B2

公开(公告)日：2020-06-16

申请号：US15545311

申请日：2016-04-11

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Tong Un Chae ,  Chan Woo Song

IPC: C12N15/52 ,  C12N15/79 ,  C12P17/10 ,  C12N9/10 ,  A61K35/742 ,  C08G69/14

Abstract: The present disclosure relates to a recombinant microorganism having a lactam production capacity from an omega-amino acid, into which a gene encoding a beta-alanine coenzyme A transferase on a microorganism which has an omega-amino acid biosynthetic metabolic pathway inherently or an omega-amino acid biosynthetic metabolic pathway is introduced, and a method for producing a variety of lactams and omega-amino acyl-CoAs using the same.The recombinant microorganism and the method for producing the lactam according to the present disclosure are useful in producing a variety of lactams such as propiolactam, 2-pyrrolidone, valerolactam, caprolactam, and heptanolactam from a variety of omega-amino acids.

公开(公告)号：US10472636B2

公开(公告)日：2019-11-12

申请号：US15556458

申请日：2016-03-17

Applicant: Korea Advanced Institute of Science and Technology

Inventor： Sang Yup Lee ,  Tong Un Chae ,  Chan Woo Song

IPC: C12N1/20 ,  C12N15/52 ,  C12N9/88 ,  C12N15/63 ,  C12P7/40 ,  C12N15/01 ,  C12P7/42 ,  C12P13/00 ,  C12P13/04 ,  C12P13/06 ,  C12R1/00

Abstract: The present invention relates to a mutant organism having the ability to produce aspartic acid derivatives, wherein a gene encoding the glyoxylate shunt regulator and a gene encoding fumarase are deleted and a gene encoding aspartase is overexpressed compared to that in a wild-type strain, and to a method for producing L-aspartic acid derivatives using the same. According to the present invention, various aspartic acid derivatives, including L-alanine, 3-aminopropionic acid, threonine, 1,3-diaminopropane, lysine, methionine, 3-hydroxypropionic acid, cadaverine, 5-aminovaleric acid, etc., can be produced by biological methods.

公开(公告)号：US10351890B2

公开(公告)日：2019-07-16

申请号：US15533273

申请日：2015-12-09

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Hannah Chung

IPC: C12P21/00 ,  A61K39/00 ,  C12N15/113

Abstract: The present invention relates to a method for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.

公开(公告)号：US20180327801A1

公开(公告)日：2018-11-15

申请号：US16027478

申请日：2018-07-05

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Hannah Chung

IPC: C12P21/00 ,  C12N15/113

CPC classification number: C12P21/00 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2330/50 ,  C12Y301/26005

Abstract: A method is described for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. By the disclosed method, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.

公开(公告)号：US20170342454A1

公开(公告)日：2017-11-30

申请号：US15533273

申请日：2015-12-09

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Hannah Chung

IPC: C12P21/00 ,  C12N15/113

CPC classification number: C12P21/00 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2330/50 ,  C12Y301/26005

Abstract: The present invention relates to a method for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene.

公开(公告)号：US11965200B2

公开(公告)日：2024-04-23

申请号：US17549757

申请日：2021-12-13

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Sang Yup Lee ,  Tae hee Han

IPC: C12P7/44 ,  C12N15/77 ,  C12R1/15

CPC classification number: C12P7/44 ,  C12N15/77 ,  C12R2001/15

Abstract: The present invention relates to a recombinant microorganism imparted with increased ability to produce glutaric acid by further introducing a gene encoding a polypeptide having glutaric acid transporter activity into a microorganism having ability to produce glutaric acid, and to a method of preparing glutaric acid using the recombinant microorganism. According to the present invention, glutaric acid used for the preparation of various compounds such as polyamide, polyurethane, 1,5-pentanediol, and 5-hydroxyvaleric acid can be biosynthesized at high yield.

公开(公告)号：US11898188B2

公开(公告)日：2024-02-13

申请号：US16962213

申请日：2019-01-15

Applicant: KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY ,  HANWHA SOLUTIONS CORPORATION

Inventor： Sang Yup Lee ,  Jae Sung Cho ,  Je Woong Kim ,  Yoo Sung Ko ,  Cindy Pricilia Surya Prabowo ,  Taehee Han ,  Euiduk Kim ,  Jae Won Choi ,  Changhee Cho ,  Jun Kyu Lee

IPC: C12P7/18 ,  C12N9/04 ,  C12N9/12 ,  C12N9/88 ,  C12N15/77 ,  C12N1/21

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/1205 ,  C12N9/88 ,  C12N15/77 ,  C12Y101/01006 ,  C12Y101/01202 ,  C12Y207/0103 ,  C12Y402/0103

Abstract: The present disclosure relates to a mutant microorganism in which a glycerol catabolic pathway and a 1,3-PDO biosynthetic pathway are introduced into a microorganism incapable of using glycerol as a carbon source, and a method of producing 1,3-PDO using the same. According to the present disclosure, it is possible to produce 1,3-PDO while growing a mutant microorganism having 1,3-PDO production ability by using the inexpensive raw material glycerol as a single carbon source. Thus, the present disclosure is useful for the economical production of 1,3-PDO.