公开(公告)号：US20130275349A1

公开(公告)日：2013-10-17

申请号：US13976967

申请日：2011-12-28

申请人： Kei Tashiro ,  Shigeru Kinoshita ,  Tomohito Yagi ,  Masakazu Nakano ,  Kazuhiko Mori ,  Yoko Ikeda ,  Morio Ueno ,  Yuichi Tokuda ,  Katsumi Yagi ,  Kengo Yoshii ,  Masahiro Fuwa

发明人： Kei Tashiro ,  Shigeru Kinoshita ,  Tomohito Yagi ,  Masakazu Nakano ,  Kazuhiko Mori ,  Yoko Ikeda ,  Morio Ueno ,  Yuichi Tokuda ,  Katsumi Yagi ,  Kengo Yoshii ,  Masahiro Fuwa

IPC分类号： G06N99/00

CPC分类号： G06N20/00 ,  C12Q1/6886 ,  G16B20/00 ,  G16B25/00 ,  G16B40/00

摘要： Provided is a technique for determining a physiological attribute in a mammal, including the onset or progression of human glaucoma, with high accuracy. The results of the determination of genotype date and the results of the determination of cytokine date are consolidated by a consolidated determination unit (114); comparison is made for determining as to which is larger, the number of Case determination procedures or the number of control determination procedures (S330); and it is determined as Case (glaucoma) when the number of Case determination procedures is larger and it is determined as Control (normal person) when the number of Control determination procedures is larger.

摘要翻译： 提供了一种用于以高精度确定哺乳动物的生理属性，包括人类青光眼的发病或进展的技术。 确定基因型日期的结果和细胞因子日期的确定结果由综合确定单位（114）合并; 比较确定哪个更大，判定程序的数量或控制确定程序的数量（S330）; 当判定判定过程的数量较多并且当控制确定过程的数量较大时被确定为控制（普通人）时被确定为病例（青光眼）。

公开(公告)号：US20110207122A1

公开(公告)日：2011-08-25

申请号：US12596462

申请日：2008-04-17

申请人： Shigeru Kinoshita ,  Kei Tashiro ,  Masakazu Nakano ,  Tomohito Yagi ,  Kazuhiko Mori ,  Yoko Ikeda ,  Takazumi Taniguchi ,  Masaaki Kageyama

发明人： Shigeru Kinoshita ,  Kei Tashiro ,  Masakazu Nakano ,  Tomohito Yagi ,  Kazuhiko Mori ,  Yoko Ikeda ,  Takazumi Taniguchi ,  Masaaki Kageyama

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6886 ,  C12Q1/6883 ,  C12Q2600/156

摘要： A method of determining the presence or the absence of a glaucoma risk, including the steps of detecting in vitro an allele and/or a genotype of a single nucleotide polymorphism which is located on a 31st base of a base sequence, in a sample from a subject, wherein the base sequence is at least one base sequence selected from the group consisting of base sequences shown in SEQ ID NOs: 203 to 752 or a complementary sequence thereto (step A), and comparing the allele and/or the genotype detected in the step A with at least one of an allele and/or a genotype, containing a high-risk allele, in the base sequences shown in SEQ ID NOs: 203 to 752 (step B). According to the method of the present invention, the level of a progressive risk of glaucoma in a sample donor can be determined by analyzing an allele or a genotype of a single nucleotide polymorphism in the present invention in the sample, so that the sample donor can take a preventive measure of glaucoma, or can receive appropriate treatments, on the basis of this risk.

摘要翻译： 一种确定青光眼风险的存在或不存在的方法，包括以下步骤：在位于碱基序列的第31位的单核苷酸多态性的体外检测等位基因和/或基因型， 其中所述碱基序列是选自SEQ ID NO：203至752所示的碱基序列或其互补序列的至少一种碱基序列（步骤A），并比较检测到的等位基因和/或基因型 在SEQ ID NO：203〜752（步骤B）所示的碱基序列中具有含有高风险等位基因的等位基因和/或基因型中的至少一种的步骤A。 根据本发明的方法，可以通过分析样品中本发明的单核苷酸多态性的等位基因或基因型来确定样品供体中青光眼进行性风险的水平，使得样品供体可以 采取预防措施的青光眼，或者可以根据这种风险接受适当的治疗。

公开(公告)号：US20130210668A1

公开(公告)日：2013-08-15

申请号：US13850453

申请日：2013-03-26

申请人： Shigeru KINOSHITA ,  Kei Tashiro ,  Masakazu Nakano ,  Tomohito Yagi ,  Kazuhiko Mori ,  Yoko Ikeda ,  Takazumi Taniguchi ,  Masaaki Kageyama

发明人： Shigeru KINOSHITA ,  Kei Tashiro ,  Masakazu Nakano ,  Tomohito Yagi ,  Kazuhiko Mori ,  Yoko Ikeda ,  Takazumi Taniguchi ,  Masaaki Kageyama

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q1/6883 ,  C12Q2600/156

摘要： A method of determining the presence or the absence of a glaucoma risk, including the steps of detecting in vitro an allele and/or a genotype of a single nucleotide polymorphism which is located on a 31st base of a base sequence, in a sample from a subject, wherein the base sequence is at least one base sequence selected from the group consisting of base sequences shown in SEQ ID NOs: 203 to 752 or a complementary sequence thereto (step A), and comparing the allele and/or the genotype detected in the step A with at least one of an allele and/or a genotype, containing a high-risk allele, in the base sequences shown in SEQ ID NOs: 203 to 752 (step B). According to the method of the present invention, the level of a progressive risk of glaucoma in a sample donor can be determined by analyzing an allele or a genotype of a single nucleotide polymorphism in the present invention in the sample, so that the sample donor can take a preventive measure of glaucoma, or can receive appropriate treatments, on the basis of this risk.

摘要翻译： 一种确定青光眼风险的存在或不存在的方法，包括以下步骤：在位于碱基序列的第31位的单核苷酸多态性的体外检测等位基因和/或基因型， 其中所述碱基序列是选自SEQ ID NO：203至752所示的碱基序列或其互补序列的至少一种碱基序列（步骤A），并比较检测到的等位基因和/或基因型 在SEQ ID NO：203〜752（步骤B）所示的碱基序列中具有含有高风险等位基因的等位基因和/或基因型中的至少一种的步骤A。 根据本发明的方法，可以通过分析样品中本发明的单核苷酸多态性的等位基因或基因型来确定样品供体中青光眼进行性风险的水平，使得样品供体可以 采取预防措施的青光眼，或者可以根据这种风险接受适当的治疗。

公开(公告)号：US08431345B2

公开(公告)日：2013-04-30

申请号：US12596462

申请日：2008-04-17

申请人： Shigeru Kinoshita ,  Kei Tashiro ,  Masakazu Nakano ,  Tomohito Yagi ,  Kazuhiko Mori ,  Yoko Ikeda ,  Takazumi Taniguchi ,  Masaaki Kageyama

发明人： Shigeru Kinoshita ,  Kei Tashiro ,  Masakazu Nakano ,  Tomohito Yagi ,  Kazuhiko Mori ,  Yoko Ikeda ,  Takazumi Taniguchi ,  Masaaki Kageyama

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6886 ,  C12Q1/6883 ,  C12Q2600/156

摘要： A method of determining the presence or the absence of a glaucoma risk, including the steps of detecting in vitro an allele and/or a genotype of a single nucleotide polymorphism which is located on a 31st base of a base sequence, in a sample from a subject, wherein the base sequence is at least one base sequence selected from the group consisting of base sequences shown in SEQ ID NOs: 203 to 752 or a complementary sequence thereto (step A), and comparing the allele and/or the genotype detected in the step A with at least one of an allele and/or a genotype, containing a high-risk allele, in the base sequences shown in SEQ ID NOs: 203 to 752 (step B). According to the method of the present invention, the level of a progressive risk of glaucoma in a sample donor can be determined by analyzing an allele or a genotype of a single nucleotide polymorphism in the present invention in the sample, so that the sample donor can take a preventive measure of glaucoma, or can receive appropriate treatments, on the basis of this risk.

摘要翻译： 一种确定青光眼风险的存在或不存在的方法，包括以下步骤：在位于碱基序列的第31位的单核苷酸多态性的体外检测等位基因和/或基因型， 其中所述碱基序列是选自SEQ ID NO：203至752所示的碱基序列或其互补序列的至少一种碱基序列（步骤A），并比较检测到的等位基因和/或基因型 在SEQ ID NO：203〜752（步骤B）所示的碱基序列中具有含有高风险等位基因的等位基因和/或基因型中的至少一种的步骤A。 根据本发明的方法，可以通过分析样品中本发明的单核苷酸多态性的等位基因或基因型来确定样品供体中青光眼进行性风险的水平，使得样品供体可以 采取预防措施的青光眼，或者可以根据这种风险接受适当的治疗。

公开(公告)号：US20130012408A1

公开(公告)日：2013-01-10

申请号：US13546674

申请日：2012-07-11

申请人： Shigeru KINOSHITA ,  Kei Tashiro ,  Masakazu Nakano ,  Tomohito Yagi ,  Kazuhiko Mori ,  Yoko Ikeda ,  Takazumi Taniguchi ,  Masaaki Kageyama

发明人： Shigeru KINOSHITA ,  Kei Tashiro ,  Masakazu Nakano ,  Tomohito Yagi ,  Kazuhiko Mori ,  Yoko Ikeda ,  Takazumi Taniguchi ,  Masaaki Kageyama

IPC分类号： C40B30/04 ,  C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q1/6883 ,  C12Q2600/156

摘要： A method of determining the presence or the absence of a glaucoma risk by detecting in vitro an allele and/or a genotype of a single nucleotide polymorphism, comparing the allele and/or the genotype detected with at least one of an allele and/or a genotype with a high-risk allele, wherein the presence of a glaucoma risk is determined in a case where the allele detected is the high-risk allele, or the presence of a glaucoma risk is determined in a case where the genotype detected is a homozygote of the genotype comprising the high-risk allele or a heterozygote when the high-risk allele complies with a dominant genetic model, or the presence of a glaucoma risk is determined in a case where the genotype detected is a homozygote of the genotype comprising the high-risk allele when the high-risk allele complies with a recessive genetic model.

摘要翻译： 通过体外检测单核苷酸多态性的等位基因和/或基因型来确定青光眼存在或不存在青光眼风险的方法，比较检测到的等位基因和/或基因型与等位基因和/或 具有高风险等位基因的基因型，其中在检测到的等位基因是高风险等位基因的情况下确定青光眼风险的存在，或者在检测到的基因型是纯合子的情况下确定青光眼风险的存在 在高风险等位基因符合显性遗传模型的情况下，包括高风险等位基因或杂合子的基因型的基因型，或者在检测到的基因型是包含高表达的基因型的纯合子的情况下，确定青光眼风险的存在 当高风险等位基因符合隐性遗传模型时，这种等位基因是错误的。

公开(公告)号：US20100196895A1

公开(公告)日：2010-08-05

申请号：US12596258

申请日：2008-04-17

申请人： Shigeru Kinoshita ,  Kei Tashiro ,  Masakazu Nakano ,  Tomohito Yagi ,  Kazuhiko Mori ,  Yoko Ikeda ,  Takazumi Taniguchi ,  Masaaki Kageyama

发明人： Shigeru Kinoshita ,  Kei Tashiro ,  Masakazu Nakano ,  Tomohito Yagi ,  Kazuhiko Mori ,  Yoko Ikeda ,  Takazumi Taniguchi ,  Masaaki Kageyama

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6886 ,  C12Q1/6883 ,  C12Q2600/156

摘要： A method of determining the presence or the absence of a glaucoma risk, including the steps of detecting in vitro an allele and/or a genotype of a single nucleotide polymorphism which is located on a 31st base of a base sequence, in a sample from a subject, wherein the base sequence is at least one base sequence selected from the group consisting of base sequences shown in SEQ ID NOs: 203 to 514 or a complementary sequence thereto (step A), and comparing the allele and/or the genotype detected in the step A with at least one of an allele and/or a genotype, containing a high-risk allele, in the base sequences shown in SEQ ID NOs: 203 to 514 (step B). According to the method of the present invention, the level of an onset risk of glaucoma in a sample donor can be determined by analyzing an allele or a genotype of a single nucleotide polymorphism in the present invention on the sample, so that the sample donor can take a preventive measure of glaucoma, or can receive appropriate treatments, on the basis of this risk.

摘要翻译： 一种确定青光眼风险的存在或不存在的方法，包括以下步骤：在位于碱基序列的第31位的单核苷酸多态性的体外检测等位基因和/或基因型， 其中所述碱基序列是选自SEQ ID NO：203至514所示的碱基序列或其互补序列的至少一种碱基序列（步骤A），并比较检测到的等位基因和/或基因型 具有SEQ ID NO：203〜514（步骤B）所示的碱基序列中的含有高风险等位基因的等位基因和/或基因型中的至少一种的步骤A。 根据本发明的方法，可以通过对样品中的本发明中的单核苷酸多态性的等位基因或基因型进行分析来确定样品供体中的青光眼的发病风险水平，从而样品供体可以 采取预防措施的青光眼，或者可以根据这种风险接受适当的治疗。